Identification and characterization of a late gene encoded by grouper iridovirus 2L (GIV‐2L)

Abstract

Grouper iridovirus (GIV) belongs to the Ranavirus genus and is one of the most important viral pathogens in grouper, particularly at the fry and fingerling stages. In this study, we identified and characterized the GIV-2L gene, which encodes a protein of unknown function. GIV-2L is 1242 bp in length, with a predicted protein mass of 46.2 kDa. It displayed significant identity only with members of the Ranavirus and Iridovirus genera. We produced mouse monoclonal antibodies against the GIV-2L protein by immunizing mice with GIV-2L-His-tag recombinant protein. By inhibiting de novo protein and DNA synthesis in GIV-infected cells, we showed that GIV-2L was a late gene during the viral replication. Finally, immunofluorescence microscopy revealed that GIV-2L protein accumulated in both the nucleus and cytoplasm of infected cells. These results offer important insights into the pathogenesis of GIV.

The Alteration of Intestinal Microbiota Profile and Immune Response in Epinephelus coioides during Pathogen Infection

“…NNV mainly attacks the central nervous system and is associated with viral encephalopathy and retinopathy (VER), vacuolation in the brain and Life 2021, 11, 99 2 of 17 eyes, abnormal swimming, dark body color and even anorexia [4,6]. Other major pathogens, such as GIV of the genus Ranavirus, also cause severe mortalities of up to 60% and lead to huge economic loss in the grouper industry [7,8]. The symptoms of infectious grouper include darker skin color, and the gills may bleed and become congested; additionally, the target organs (spleen and head kidney) become swollen [3,9].…”

Imunobiológicos para o diagnóstico laboratorial de ranavirose: clonagem e expressão do gene MCP de isolado brasileiro de >i<Ranavirus>/i< e produção de anticorpos policlonais anti-proteína MCP recombinante

“…Além dos anticorpos policlonais, anticorpos monoclonais têm sido obtidos para a detecção e caracterização de Ranavirus, principalmente na avaliação da expressão de genes durante a replicação viral por ensaios de imunofluorescência (LIN et al, 2014;LIN et al, 2015;CHEN et al, 2016). Lin et al (2014) utilizaram 100 µg da proteína recombinante do MCP (GIV-MCP-His) de Grouper iridovirus (GIV) para imunizar camundongos, sendo coletados os soros policlonais e preparados os hibridomas e, na sequência, selecionados por ELISA (de 73 clones, 6 foram positivos).…”