Identification and Characterization of a High Efficiency Aniline Resistance and Degrading Bacterium MC-01

Abstract

Biodegradation is one of the important methods for the treatment of industrial wastewater containing aniline. In this paper, a degrading bacterium named MC-01, which could survive in high concentration aniline wastewater, was screened from industrial wastewater containing aniline and sludge. MC-01 was preliminarily identified as Ochrobactrum sp. based on the amplified 16S rDNA gene sequence and Biolog system identification. MC-01 was highly resistant to aniline. After 24-h culture under aniline concentration of 6500 mg/L, the amount of bacterium survived still remained 0.05 × 10 CFU/mL. Experiments showed that there was no coupling expression between the growth of MC-01 and aniline degradation. The optimum growth conditions in LB culture were pH 6.0, 30 °C of temperature, and 4% of incubation amount, respectively. And the optimum conditions of aniline degradation of MC-01 were pH 7.0, 45 °C of temperature, and 3.0% of salt concentration, respectively. The degradation rate of MC-01 (48 h) in different aniline concentrations (200~1600 mg/L) was stable under the optimum conditions, which could reach more than 75%.

Identification and Characterization of a Novel Plasmid-Encoded Laccase-Like Multicopper Oxidase from Ochrobactrum sp. BF15 Isolated from an On-Farm Bio-Purification System

“…In addition, plasmid conjugation machinery has been identified in Ochrobactrum plasmids ( 20 , 42 ), and horizontal gene transfer by conjugation was demonstrated in this genus ( 47 , 48 ). Ochrobactrum species was previously found to degrade a wide spectrum of recalcitrant and xenobiotic compounds, such as organophosphates ( 49 ), tetrachloroethene ( 50 ), dichloro-diphenyl-trichloroethane (DDT) ( 51 ), 2,4-dichlorophenoxyacetic acid, endosulfan ( 52 ) and aniline ( 53 ), among many others. Interestingly, ligninolytic activity was also reported from Ochrobactrum species ( 39 , 41 ).…”