1998
DOI: 10.1074/jbc.273.17.10325
|View full text |Cite
|
Sign up to set email alerts
|

Identification and Characterization of a Novel Cap-binding Protein from Arabidopsis thaliana

Abstract: Cap-binding proteins specifically bind to the 7-methyl guanosine (m 7 G) functional group at the 5 end of eukaryotic mRNAs. A novel Arabidopsis thaliana protein has been identified that has sequence similarity to capbinding proteins but is clearly a different form of the protein. The most obvious primary sequence difference is the substitution of two of the eight conserved tryptophan residues with other aromatic amino acids in the novel protein. Analogous forms of this novel protein appear to be present in oth… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

1
89
0
1

Year Published

2001
2001
2016
2016

Publication Types

Select...
4
3
1

Relationship

0
8

Authors

Journals

citations
Cited by 86 publications
(91 citation statements)
references
References 29 publications
1
89
0
1
Order By: Relevance
“…It has been suggested that the plant cell might use the activity of different cap-binding factors as a discriminatory mechanism in selection of the RNAs for translation (27). This is exemplified by the fact that eIFiso4E has a higher affinity for hypermethylated cap structures than eIF4E, and that the nCBP has a higher affinity for the cap than eIF4E and eIFiso4E, but a lower activity in stimulating translation (10). The latter example shows a similarity with the TED-binding factors, as p30 appears to have a lower affinity for TED than p28, but a higher capacity to stimulate translation.…”
Section: Discussionmentioning
confidence: 96%
See 2 more Smart Citations
“…It has been suggested that the plant cell might use the activity of different cap-binding factors as a discriminatory mechanism in selection of the RNAs for translation (27). This is exemplified by the fact that eIFiso4E has a higher affinity for hypermethylated cap structures than eIF4E, and that the nCBP has a higher affinity for the cap than eIF4E and eIFiso4E, but a lower activity in stimulating translation (10). The latter example shows a similarity with the TED-binding factors, as p30 appears to have a lower affinity for TED than p28, but a higher capacity to stimulate translation.…”
Section: Discussionmentioning
confidence: 96%
“…First, in yeast extracts it was shown that both eIF4E and the nuclear cap-binding complex stimulate translation via eIF4G binding (8). Secondly, specifically in plants, three cap-binding factors eIF4E, eIFiso4E and nCBP have been described that stimulate translation in vitro (9,10). It has been suggested that the plant cell might use the activity of different cap-binding factors as a discriminatory mechanism in selection of the RNAs for translation (27).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In mammals, there are three members of the eIF4E family: eIF4E (eIF4E-1), 4EHP (eIF4E-2), and eIF4E-3 (Rom et al 1998;Joshi et al 2004). Based on sequence alignments, h4EHP, together with nCBP from plants (Ruud et al 1998), IF4E-4 from Caenorbiditis elegans and d4EHP (deIF4E-8) from Drosophila melanogaster are members of the eIF4E-2 class (Joshi et al 2005). Members of this class possess mainly Tyr/Phe in the position corresponding to Trp43 and Tyr in the position of Trp56 of human eIF4E (Joshi et al 2005).…”
Section: Introductionmentioning
confidence: 99%
“…The presence and effects of such interactions have been presumed in the interpretation of many biological functions of the 7-methylguanosine, other alkylated nucleic acid bases and their nucleotide derivatives. One example is the eukaryotic mRNA with a special CAP structure (see [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55] and the references therein) (the 5'cap is found on the end of mRNA molecule and consists of a guanine nucleotide connected to the mRNA via an unusual 5' to 5' triphosphate linkage). This guanosine is methylated on the 7 position directly after capping in vitro by a methyl transferase.…”
Section: Introductionmentioning
confidence: 99%