Identification and characterization of a pyridoxal 5′-phosphate phosphatase in the silkworm (Bombyx mori)

Huang, ShuoHao; Han, Cuiping; Ma, Zhongyou; Zhou, Jie; Zhang, Jianyun; Huang, LongQuan

doi:10.1016/j.cbpb.2017.01.003

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…Although it is known that the results of in vitro and in vivo tests may differ, and it is possible that the potential resistance of E. mundtii to the antibiotic reduced the competitiveness of E. faecalis. In general, the importance of enterococci for the host can be quite broad and may include both their probiotic role [2,[59][60][61] and their ability to produce antimicrobial peptides necessary to suppress the development of various infections [33]. However, it should be noted that studies addressing the effect of antibiotics on the insect microbiota were performed using insects up to the 6th generation (depending on a certain study) (Drosophila nigrosparsa) [5,9].…”

Section: Discussionmentioning

confidence: 99%

“…The antimicrobial substances synthesized by the microbiota cause microflora depletion and the emergence of microorganisms resistant to these substances, as well as alter the physiological processes in the host [22,62]. E. mundtii frequently isolated from the guts of various insects acts as a probiotic in the gut of mill moth Ephestia kuehniella [59]; it affects the resistance to organophosphate insecticides in the silkworm Bombyx mori [2,60] or antagonizes the action of entomopathogenic bacteria in the beetle Tribolium castaneum [59]. Meanwhile, the xenobiotic properties and toxic effects of antibiotics could alter both the immune reactions and enzymatic activities in various organs and tissues.…”

Section: Discussionmentioning

confidence: 99%

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A Diet with Amikacin Changes the Bacteriobiome and the Physiological State of Galleria mellonella and Causes Its Resistance to Bacillus thuringiensis

Polenogova,

Klementeva,

Kabilov

et al. 2023

Insects

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Environmental pollution with antibiotics can cause antibiotic resistance in microorganisms, including the intestinal microbiota of various insects. The effects of low-dose aminoglycoside antibiotic (amikacin) on the resident gut microbiota of Galleria mellonella, its digestion, its physiological parameters, and the resistance of this species to bacteria Bacillus thuringiensis were investigated. Here, 16S rDNA analysis revealed that the number of non-dominant Enterococcus mundtii bacteria in the eighteenth generation of the wax moth treated with amikacin was increased 73 fold compared to E. faecalis, the dominant bacteria in the native line of the wax moth. These changes were accompanied by increased activity of acidic protease and glutathione-S-transferase in the midgut tissues of larvae. Ultra-thin section electron microscopy detected no changes in the structure of the midgut tissues. In addition, reduced pupa weight and resistance of larvae to B. thuringiensis were observed in the eighteenth generation of the wax moth reared on a diet with amikacin. We suggest that long-term cultivation of wax moth larvae on an artificial diet with an antibiotic leads to its adaptation due to changes in both the gut microbiota community and the physiological state of the insect organism.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Diet with Amikacin Changes the Bacteriobiome and the Physiological State of Galleria mellonella and Causes Its Resistance to Bacillus thuringiensis

Polenogova,

Klementeva,

Kabilov

et al. 2023

Insects

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“…In this way, 12 genes were obtained, and the transcriptional regulation of miR6498-5p on these genes was detected. As a phosphatase, PLPP has an in vitro specificity to dephosphorylate pyridoxal 5 -phosphate (PLP) that acts as co-factor in more than 140 different enzyme reactions [34][35][36] and is especially involved in amino acid transport and metabolism. Multiple experiments demonstrated that miR6498-5p could target and negatively regulate BmPLPP2 and thereby suppress the proliferation of N. bombycis (Figures 5 and 6).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori

Dong

Deng

et al. 2021

JoF

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As microRNAs (miRNAs) are important expression regulators of coding RNA, it is important to characterize their role in the interaction between hosts and pathogens. To obtain a comprehensive understanding of the miRNA alternation in Bombyx mori (B. mori) infected with Nosema bombycis (N. bombycis), RNA sequencing and stem-loop qPCR were conducted to screen and identify the significantly differentially expressed miRNAs (DEmiRNAs). A total of 17 such miRNAs were identified in response to N. bombycis infection, among which miR6498-5p efficiently inhibited the proliferation of N. bombycis in BmE-SWU1 (BmE) cells by downregulating pyridoxal phosphate phosphatase 2 (BmPLPP2). In addition, a fluorescence in situ hybridization (FISH) assay showed that miR6498-5p was located in the cytoplasm of BmE cells, while it was not found in the schizonts of N. bombycis. Further investigation of the effect of BmPLPP2 on the proliferation of schizonts found that the positive factor BmPLPP2 could facilitate N. bombycis completing its life cycle in cells by overexpression and RNAi of BmPLPP2. Our findings offer multiple new insights into the role of miRNAs in the interaction between hosts and microsporidia.

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Beneficial efficacy and mode of action of probiotic Bacillus subtilis SWL−19 on the silkworm (Bombyx mori L.)

Li,

Xiao,

Leng

et al. 2024

Symbiosis

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Identification and characterization of a pyridoxal 5′-phosphate phosphatase in the silkworm (Bombyx mori)

Cited by 3 publications

References 24 publications

A Diet with Amikacin Changes the Bacteriobiome and the Physiological State of Galleria mellonella and Causes Its Resistance to Bacillus thuringiensis

A Diet with Amikacin Changes the Bacteriobiome and the Physiological State of Galleria mellonella and Causes Its Resistance to Bacillus thuringiensis

MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 in Bombyx mori

Beneficial efficacy and mode of action of probiotic Bacillus subtilis SWL−19 on the silkworm (Bombyx mori L.)

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