Identification and Characterization of Abiotic Stress–Responsive NF-YB Family Genes in Medicago

Abstract

Nuclear factor YB (NF-YB) are plant-specific transcription factors that play a critical regulatory role in plant growth and development as well as in plant resistance against various stresses. In this study, a total of 49 NF-YB genes were identified from the genomes of Medicago truncatula and Medicago sativa. Multiple sequence alignment analysis showed that all of these NF-YB members contain DNA binding domain, NF-YA interaction domain and NF-YC interaction domain. Phylogenetic analysis suggested that these NF-YB proteins could be classified into five distinct clusters. We also analyzed the exon–intron organizations and conserved motifs of these NF-YB genes and their deduced proteins. We also found many stress-related cis-acting elements in their promoter region. In addition, analyses on genechip for M. truncatula and transcriptome data for M. sativa indicated that these NF-YB genes exhibited a distinct expression pattern in various tissues; many of these could be induced by drought and/or salt treatments. In particular, RT-qPCR analysis revealed that the expression levels of gene pairs MsNF-YB27/MtNF-YB15 and MsNF-YB28/MtNF-YB16 were significantly up-regulated under NaCl and mannitol treatments, indicating that they are most likely involved in salt and drought stress response. Taken together, our study on NF-YB family genes in Medicago is valuable for their functional characterization, as well as for the application of NF-YB genes in genetic breeding for high-yield and high-resistance alfalfa.

Ammonium Uptake, Mediated by Ammonium Transporters, Mitigates Manganese Toxicity in Duckweed, Spirodela polyrhiza

“…Among prominent conservative cis -elements present within the 500 bp region upstream of translation start in all analyzed Spirodela AMTs are the TATA-box, a binding site for the general eukaryotic transcription factor TBP [ 54 ]. All AMT promoters also contain another general eukaryotic cis- element CCAAT-box [ 55 ], a binding site for NF-Y (nuclear factor Y) [ 105 ], involved in many important growth and developmental processes [ 106 ], including stress and hormone responses [ 107 , 108 ].…”

Transcription factors NF-YB involved in embryogenesis and hormones responses in Dimocarpus Longan Lour

“…Arabidopsis thaliana protein sequence data were downloaded from https://www.arabidopsis.org/, Gossypium spp. protein sequence data were downloaded from https://www.cottongen.org/, Oryza sativa protein sequence data were downloaded from https:// rapdb.dna.affrc.go.jp/, Litchi chinensis Sonn (Hu et al, 2022) genome data were downloaded from https://data.mendeley.com/ datasets/kggzfwpdr9/1, Medicago sativa L. (Du et al, 2022) genome data were downloaded from https://figshare.com/articles/dataset/ Medicago_sativa_genome_and_annotation_files/12623960, Larix kaempferi (Li et al, 2021;Li et al, 2023) genome data were download ed from NCB I (NCBI B ioProject nu mber: PRJNA648500), and 'Jidanben' longan (CRA004281) (Wang et al, 2022) genome data were downloaded from https:// ngdc.cncb.ac.cn/. MEGA5.05 software was used to construct the phylogenetic tree, and the neighbor-joining method (NJ) was used to set the bootstrap value to 1,000 for repetition.…”

Genome-Wide Identification and Characterization of the Msr Gene Family in Alfalfa under Abiotic Stress

“…Using the genome and annotation information of the "Xinjiang Daye", 15 upstream 2000 bp promoter sequences of MsMsr genes were extracted via TBtools. In previous studies, the online program PlantCARE 10 (http://bioinformatics.psb.ugent.be/webtools/ plantcare/html) (accessed on 7 October 2022) has been widely used for promoter element analysis [53,54]. Therefore, we chose PlantCARE 10 along with New PLACE (https://www.…”