Identification and Characterization of Alternatively Transcribed Form of Peroxiredoxin IV Gene That Is Specifically Expressed in Spermatids of Postpubertal Mouse Testis

Yim, Sun Hee; Kim, Yoo-Jin; Oh, Sejong; Fujii, Junichi; Zhang, Yan; Gladyshev, Vadim N.; Rhee, Sue Goo

doi:10.1074/jbc.m111.257220

Cited by 25 publications

(21 citation statements)

References 49 publications

(68 reference statements)

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“…Prdx4 is on the X chromosome and produces two forms of alternative transcripts [31]. Each of them uses a different exon 1 (exon 1A and exon 1B) [86]. All tissues, including the testis, express Prdx4 mRNAs transcribed from exon 1B, which encodes the cleavable N-terminal signal sequence, whereas the testis produces a testis-specific form of Prdx4 mRNAs transcribed from exon 1A [86].…”

Section: Genetics and Knockout Mouse Strainsmentioning

confidence: 99%

“…Each of them uses a different exon 1 (exon 1A and exon 1B) [86]. All tissues, including the testis, express Prdx4 mRNAs transcribed from exon 1B, which encodes the cleavable N-terminal signal sequence, whereas the testis produces a testis-specific form of Prdx4 mRNAs transcribed from exon 1A [86]. PRDX4 is predominantly present in the ER and secreted to extracellular space [87,88].…”

Section: Genetics and Knockout Mouse Strainsmentioning

confidence: 99%

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Knockout Mouse Models for Peroxiredoxins

Lee

2020

Antioxidants

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Peroxiredoxins (PRDXs) are members of a highly conserved peroxidase family and maintain intracellular reactive oxygen species (ROS) homeostasis. The family members are expressed in most organisms and involved in various biological processes, such as cellular protection against ROS, inflammation, carcinogenesis, atherosclerosis, heart diseases, and metabolism. In mammals, six PRDX members have been identified and are subdivided into three subfamilies: typical 2-Cys (PRDX1, PRDX2, PRDX3, and PRDX4), atypical 2-Cys (PRDX5), and 1-Cys (PRDX6) subfamilies. Knockout mouse models of PRDXs have been developed to investigate their in vivo roles. This review presents an overview of the knockout mouse models of PRDXs with emphases on the biological and physiological changes of these model mice.

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Section: Genetics and Knockout Mouse Strainsmentioning

confidence: 99%

Section: Genetics and Knockout Mouse Strainsmentioning

confidence: 99%

Knockout Mouse Models for Peroxiredoxins

Lee

2020

Antioxidants

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“…Among the 6 PRDX family members, PRDX4 appears to play a role in spermatogenesis because its deficiency delays sexual maturation and makes testicular cells vulnerable to heat stress [75]. A testis-specific variation of PRDX4 is expressed and may function in the spermatogenic process [76,77]. Declining the redox state increases the oxidation of PRDX in the sperm and appears to cause male infertility [78][79][80].…”

Section: Ros and Redox Reactions Involved In Fertilizationmentioning

confidence: 99%

Oxidative stress and redox regulation of gametogenesis, fertilization, and embryonic development

Tsunoda

Kimura

Fujii

2013

Reprod Medicine & Biology

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Oxidative stress caused by elevated reactive oxygen species (ROS) is one of the predominant causes of both male and female infertility. Oxidative stress conditions cause either cell death or senescence by oxidation of cellular molecules including nucleic acid, proteins, and lipids. It is particularly important to minimize oxidative stress when in vitro fertilization is performed for the purpose of assisted reproduction. The problems associated with assisted reproductive technology are becoming evident, and it is now the time to clarify its mechanisms and cope with them. On the other hand, the beneficial roles of ROS, such as intracellular signaling, have become evident. The antithetical functions of ROS make it more difficult to overcome the problems caused by oxidative stress. Despite the difficulty in understanding mammalian reproduction, the mechanisms and problems can be gradually unveiled by advanced technology such as genetic modification of animals.

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“…However, different from catalase, an electron donor is required for the PRDX4-mediated peroxidase reaction. While hyperoxidation occurs dominantly to PRDX1, 2, and 3, hyperoxidized PRDX4 is rarely detected in vivo [32,46]. Because hyperoxidation occurs during catalytic reaction of PRDX with Trx as the preferred electron donor, this could be explained by an insufficient electron-donating system in the ER that includes the Trx-Trx reductase system and the NADPH generating system.…”

Section: Roles Of Prdx4 In the Ermentioning

confidence: 91%

“…Now the low-mobility form has been identified as the variant PRDX4t that is transcribed from the upstream promoter/exon 1 only in sexually mature testes (Fig. 4A) [46]. Thus, two splice variants, the systemically expressed form with a hydrophobic signal peptide and the testis-specific form without the signal peptide, are transcribed from the PRDX4 gene, which is localized in the X chromosome [15].…”

Section: A Testis-specific Prdx4 Variant and A Hypothetical Rolementioning

confidence: 99%