Identification and characterization of Borrelia antigens as potential vaccine candidates against Lyme borreliosis

Borrelia burgdorferisurface-located Lmp1 protein processed into region-specific polypeptides that are critical for microbial persistence

“…In fact, Lmp1 is amongst a handful of spirochete gene-products that is known to undergo proteolytic or other modes of protein maturation events. Lmp1 is a conserved, chromosomally-encoded protein (Fraser et al ., 1997) required for establishment of persistence infection in mammals, or transmission from infected ticks to murine hosts, and also predicted to be a vaccine candidate against diverse B. burgdorferi species (Poljak et al ., 2012). Therefore, further understanding of the maturation process of a potential multi-functional protein like Lmp1, for its role in the host-pathogen interaction or other unknown functions, will enrich our knowledge of intriguing biology and infectivity of spirochetes.…”

Mechanisms of Immune and Cytokine Regulation in Pathogenesis of Ixodes Tick-Borne Borreliosis

“…Иммуноопосредованные механизмы играют важную роль в патогенезе ИКБ [6,9,11]. Актуальной задачей при-кладных клинических исследований в области клещевых инфекций является поиск ключевых факторов иммуноре-гуляции, определяющих развитие тех или иных клиниче-ских форм ИКБ [4,8,10,13].…”

“…Имеющиеся сведения об иммунопатогенезе различных клинических форм ИКБ и его сочетанного течения с клещевым энцефалитом (КЭ) весьма противоречивы. Ряд исследований свидетельству-ет о преобладании T х2 (гуморального) типа иммунного ответа при остром течении заболевания [8][9][10][11]. В то же время существуют доказательства значимого участия в иммунопатогенезе T х1 (клеточного) типа иммунного от-вета, в результате которого происходит непосредственное стимулирующее действие на фагоцитоз и цитотоксиче-ские лимфоциты [4,[12][13][14].…”

Recent contributions in the field of the recombinant expression of disulfide bonded protein in bacteria

“…Although the secretion mechanisms are not always well characterized [13], the systems set for accumulating the target proteins in the cell supernatant never seemed as popular as recently. E. coli alpha-hemolysin type I and autotransporter secretion systems [14,15], Salmonella flagellar type III [16] as well as the combinations leading to target protein cell display in different bacteria [17-22] underline the present available methodological variety. In particular, the display systems combined to flow-cytometry and magnetic cell sorting seem to be very promising for large-scale screenings of polypeptides with desired characteristics.…”

“…In particular, the display systems combined to flow-cytometry and magnetic cell sorting seem to be very promising for large-scale screenings of polypeptides with desired characteristics. In such a way, antigenic and curative peptides to be used for vaccination have been identified [22] and E. coli displaying both Mycobacterium tuberculosis and Salmonella enterica vaccine targets have been foreseen as live vaccines [18,20]. Finally, bacteria displaying recombinant antibodies were directly spotted on chips for preparing effective diagnostic microarrays without the necessity of any purification step [23].…”