Identification and Characterization of Chalcone Synthase Gene Family Members in Nicotiana tabacum

Chen, Shuai; Pan, Xuhao; Li, Yiting; Cui, Lijie; Zhang, Yinchao; Zhang, Zhiming; Pan, Guangtang; Yang, Jun; Cao, Peijian; Yang, Aiguo

doi:10.1007/s00344-016-9646-6

Cited by 21 publications

(16 citation statements)

References 43 publications

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“…The PCR product was digested with SacI/KpnI and cloned into the SacI/ KpnI-digested pCAMBIA1305 plasmid under the control of the CaMV 35S promoter. In order to construct the overexpression vector NtCHS-pCAMBIA1305, the coding region (CDS) of NtCHS (Chen et al, 2017) was amplified using primers ( Table S1). The PCR product was digested with SacI/KpnI and cloned into the SacI/ KpnI-digested pCAMBIA1305 plasmid under the control of the CaMV 35S promoter.…”

Section: Generation Of Transgenic Plantsmentioning

confidence: 99%

NtMYB12 Positively Regulates Flavonol Biosynthesis and Enhances Tolerance to Low Pi Stress in Nicotiana tabacum

et al. 2020

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Phosphorus (P) is an essential macronutrient for plant growth and development. The concentration of flavonol, a natural plant antioxidant, is closely related to phosphorus nutritional status. However, the regulatory networks of flavonol biosynthesis under low Pi stress are still unclear. In this study, we identified a PFG-type MYB gene, NtMYB12, whose expression was significantly up-regulated under low Pi conditions. Overexpression of NtMYB12 dramatically increased flavonol concentration and the expression of certain flavonol biosynthetic genes (NtCHS, NtCHI, and NtFLS) in transgenic tobacco. Moreover, overexpression of NtMYB12 also increased the total P concentration and enhanced tobacco tolerance of low Pi stress by increasing the expression of Pht1-family genes (NtPT1 and NtPT2). We further demonstrated that NtCHS-overexpressing plants and NtPT2-overexpressing plants also had increased flavonol and P accumulation and higher tolerance to low Pi stress, showing a similar phenotype to NtMYB12-overexpressing transgenic tobacco under low Pi stress. These results suggested that tobacco NtMYB12 acts as a phosphorus starvation response enhancement factor and regulates NtCHS and NtPT2 expression, which results in increased flavonol and P accumulation and enhances tolerance to low Pi stress.

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Section: Generation Of Transgenic Plantsmentioning

confidence: 99%

NtMYB12 Positively Regulates Flavonol Biosynthesis and Enhances Tolerance to Low Pi Stress in Nicotiana tabacum

et al. 2020

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“…The enzyme 4-coumaroyl-CoA ligase (4CL) is ubiquitous in plants and plays a central role in the biosynthesis of phenylpropanoids, such as lignins, flavonoids, and coumarins . Chalcone synthase (CHS) plays diverse and crucial roles in the secondary metabolism of plants, such as producing flavonoids and ultraviolet protectants, and it is the first-step enzyme in the flavonoid biosynthetic pathway. Dihydroflavonol 4-reductase (DFR) is regarded as the central enzyme of the flavonoid pathway and controls the direction of carbon flux and regulates various stereochemical features of flavan-3-ols and related compounds. , Leucoanthocyanidin reductase (LAR) is an essential reductase and the key enzyme responsible for the catalysis of catechin monomers in the flavonoid pathway .…”

Section: Introductionmentioning

confidence: 99%

Metabolic Flux Enhancement and Transcriptomic Analysis Displayed the Changes of Catechins Following Long-Term Pruning in Tea Trees (Camellia sinensis)

Sun

Zhang²,

et al. 2018

J. Agric. Food Chem.

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The tea tree is a perennial woody plant, and pruning is one of the most crucial cultivation measurements for tea plantation management. To date, the relationship between long-term pruning and metabolic flux enhancement in tea trees has not been studied. In this research, 11-year-old pruned tea trees from four different cultivars were randomly selected for transcriptome analysis and characteristic secondary metabolite analysis together with controls. The findings revealed that epigallocatechin gallate (EGCG) accumulation in pruned tea trees was significantly higher than that in unpruned tea trees. SCPL1A expression (encoding a class of serine carboxypeptidase), which has been reported to have a catalytic ability during EGCG biosynthesis, together with LAR, encoding leucoanthocyanidin reductase, was upregulated in the pruned tea trees. Moreover, metabolic flux enhancement and transcriptome analysis revealed low EGCG accumulation in the leaves of unpruned tea trees. Because of the bitter and astringent taste of EGCG, these results provide a certain understanding to the lower bitterness and astringency in teas from "ancient tea trees", growing in the wild with no trimming, than teas produced from pruned plantation trees.

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“…Several recent studies have shown that MeJA treatment can increase the contents of flavonoids and terpenes in several different species, including Hypericum perforatum [59], Norway spruce [60], Camellia sinensis [61], and G. biloba [62]. Moreover, MeJA treatment was shown to induce CHS gene expression in Coleus forskohlii [63], Carthamus tinctorius [64], and Nicotiana tabacum [65], among others. After the citrus leaves were treated with MeJA, the expression of the two CHS genes decreased but the expression increased after 24 h [66].…”

Section: Discussionmentioning

confidence: 99%

Selection of Suitable Reference Genes Based on Transcriptomic Data in Ginkgo biloba under Different Experimental Conditions

Zhou

Yang

et al. 2020

Forests

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Ginkgo biloba, a deciduous tree species in the Ginkgo family, has a long history of cultivation in China and is widely used in garden landscapes, medicine, food, and health products. However, few reports have focused on the systematic selection of optimal reference genes based on transcriptomic data in G. biloba. The purpose of our research was to select an internal reference gene suitable for different experimental conditions from thirteen candidate reference genes by the delta cycle threshold (ΔCt) method, geNorm, BestKeeper, NormFinder, and RefFinder programs. The reference genes were used for gene expression analyses of Ginkgo biloba. These results showed that elongation factor 1(EF1) and ubiquitin (UBI) were the best choices for samples of different ginkgo genotypes. The expression of UBI and HAS28 presented the most stable at different developmental stages of ginkgo, and EIF3I and RPII were considered as suitable reference genes in different tissues of ginkgo. For methyl jasmonate (MeJA) treatment, ACA and ACT were identified as the optimal reference genes. For cold stress treatment, RPII and EIF4E were chosen for the gene expression normalizations. HAS28 and GAPDH presented the most stable expression for the heat treatment. To validate the above results, a chalcone synthase gene (GbCHS) in ginkgo was amplified by quantitative real-time polymerase chain reaction (qRT-PCR). Our results provide different suitable reference genes for further gene expression studies in ginkgo.

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Identification and Characterization of Chalcone Synthase Gene Family Members in Nicotiana tabacum

Cited by 21 publications

References 43 publications

NtMYB12 Positively Regulates Flavonol Biosynthesis and Enhances Tolerance to Low Pi Stress in Nicotiana tabacum

NtMYB12 Positively Regulates Flavonol Biosynthesis and Enhances Tolerance to Low Pi Stress in Nicotiana tabacum

Metabolic Flux Enhancement and Transcriptomic Analysis Displayed the Changes of Catechins Following Long-Term Pruning in Tea Trees (Camellia sinensis)

Selection of Suitable Reference Genes Based on Transcriptomic Data in Ginkgo biloba under Different Experimental Conditions

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