Identification and Characterization of Class 1 Integron Resistance Gene Cassettes among <i>Salmonella</i> Strains Isolated from Healthy Humans in China

Zhang, Hongmei; Shi, Lei; Lin, Li; Guo, Siyuan; Zhang, Ximei; Yamasaki, Shinji; Miyoshi, Shin Ichi; Shinoda, Sumió

doi:10.1111/j.1348-0421.2004.tb03473.x

Cited by 72 publications

(69 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This observation is similar to what has been reported in a previous study where class 1 integron was not always involved in the resistance of E. coli isolates to antimicrobial agents . However integrons have been often associated with broad antibiotic resistance, even if they do not encode multiple drug resistant determinants (Zhang et al, 2004). This was also evident in our study as not all integron bearing strains expressed resistance to antibiotics.…”

Section: Salmonella From Animals 411 Salmonella In Feedlot Cattlesupporting

confidence: 65%

“…This was also evident in our study as not all integron bearing strains expressed resistance to antibiotics. Additionally, it is possible that our PCR analysis as designed in this www.intechopen.com study missed some large amplicons and most especially integron 2, which contains some gene cassettes encoding antibiotic resistance (Zhang et al, 2004 (Orman,et al 2002;Sallen et al, 1995). It has been reported (Collis, et al, 2002) that classes 1 and 2 are most common in resistant bacteria, and the mobility of these integrons was undoubtedly important in facilitating their spread into many different bacterial species.…”

Section: Salmonella From Animals 411 Salmonella In Feedlot Cattlementioning

confidence: 92%

See 1 more Smart Citation

Antimicrobial Drug Resistance and Molecular Characterization of Salmonella Isolated from Domestic Animals, Humans and Meat Products

Margaret.¹,

Doetkott²

2012

Salmonella - A Dangerous Foodborne Pathogen

View full text Add to dashboard Cite

Section: Salmonella From Animals 411 Salmonella In Feedlot Cattlesupporting

confidence: 65%

Section: Salmonella From Animals 411 Salmonella In Feedlot Cattlementioning

confidence: 92%

Antimicrobial Drug Resistance and Molecular Characterization of Salmonella Isolated from Domestic Animals, Humans and Meat Products

Margaret.¹,

Doetkott²

2012

Salmonella - A Dangerous Foodborne Pathogen

View full text Add to dashboard Cite

“…The isolates were screened for intI 1 gene. Primers used in the PCR are listed in the Table 1 [20]. PCR was performed in a 25 μl volume with 1X PCR buffer, 1.5mM MgCl2, 200 μM dNTP mix, 0.6U Taq DNA polymerase, 12.5pmol Int-1U and Int-1D primers and 2 µl of template DNA.…”

Section: Investigation Of Class 1 Integronsmentioning

confidence: 99%

A Study on Class I Integrons and Antimicrobial Resistance among Clinical Staphylococci Isolates from a Turkish Hospital

Güney¹

2014

Clin Microbiol

View full text Add to dashboard Cite

Purpose: Methicillin-resistant staphylococci (MRS) are increasingly significant in the hospital setting due to multidrug resistance. Integrons are genetic elements that can play a role in the dissemination of antimicrobial resistance. While their role is well established in gram-negative bacteria, less is known about the presence of integrons in gram-positive bacteria. Our aim was to investigate the presence of class 1 integrons in clinical MRS isolates. Methods:One hundred clinical MRS isolates were included in the study. Identification of MRS isolates was confirmed by multiplex PCR in which staphylococcal 16S rRNA, nuc and mecA genes-specific primers were used. The presence of class 1 integron was investigated by PCR with intI 1-specific primers.Results: All methicillin-resistant Staphylococcus aureus (MRSA) isolates were positive for 16S rRNA, nuc and mecA genes. All methicillin-resistant coagulase negative staphylococci (MRCoNS) were positive for 16S rRNA and mecA, and negative for nuc gene. The existence of class 1 integron was not detected in all the isolates tested. Conclusions: Class 1 integron positive clinicial MRS strains have only been shown in a few studies from East Asia. Further studies are needed in order to determine the significance of integrons in staphylococci and their role in DNA transfer between gram-positive and gram-negative bacteria.

show abstract

“…17 In a multinational study in Denmark, Germany, Italy, Spain and the United States and another study in France, thus confirmed the spread and persistence of this multidrug-resistant for epidemic strain of S. enterica serotype typhimurium. 18,19 Six out of ten strains contained plasmids ranging from 140-62 MDa and the result has been shown in 20 Class I integrons are the most extensively studied integrons, which are thought to be a major factor implicated in the dissemination of antibiotic resistance. Class I integron contained a single gene cassette dfrVII or aadA1confering resistance to trimethoprim or spectinomycin and streptomycin respectively.…”

Section: Resultsmentioning

confidence: 99%

Antibiotic Resistance, Plasmids and Integron Profile of Salmonella Species Isolated from Poultry Farm and Patients

Begum

Mannan

Ahmed

2017

Dhaka Univ. J. Pharm. Sci

View full text Add to dashboard Cite

A total of ten selected strains of Salmonella species, five from the environment of poultry farm and five from patients were included in this study. All strains were isolated and identified by using selective media, standard biochemical and serological tests. Antimicrobial susceptibility tests were performed by disc diffusion method using twelve commercial antibiotic discs of aztreonam, ceftriaxone, kanamycin, ciprofloxacin, gentamycin, chloramphenicol, ampicillin, erythromycin, cephalexin, tetracycline, cotrimoxazole and nalidixic acid. Plasmid profile and integron gene detection were conducted by Kado-Liu method and PCR, respectively. Only aztreonam, ceftriaxone, kanamycin and gentamycin were shown to be inhibitory to all strains. However, ciprofloxacin, chloramphenicol, amoxicilin, erythromycin, cephalexin, tetracycline, cotrimoxazole and nalidixic acid revealed different degrees of resistance pattern against environmental and clinical strains. Analysis of plasmid demonstrated that three environmental strains contained both plasmids of 140 Mda and 62 Mda. On the other hand, another three strains, one environmental and two clinical isolate only contained 140 Mda plasmid. All the plasmid containing strains (140 Mda and 62 Mda) exhibited same type of drug resistance pattern, whereas strains containing the 140 Mda plasmid only did not show similar type of resistance pattern. Therefore, no correlation was found between plasmid containing strains and drug resistance. Four environmental strains were also found to be positive for the class I integron and one clinical isolated was positive for class I integron gene conferring resistance to common antibiotics. However, none of the strains were found to contain class II integron. Therefore, the present study demonstrated that both environmental and clinical strains contain both large to middle size plasmids and integron I but not integron II. The plasmid and integron I containing strains experienced resistance to different antibiotics, used in the experiments.

show abstract

Identification and Characterization of Class 1 Integron Resistance Gene Cassettes among Salmonella Strains Isolated from Healthy Humans in China

Cited by 72 publications

References 24 publications

Antimicrobial Drug Resistance and Molecular Characterization of Salmonella Isolated from Domestic Animals, Humans and Meat Products

Antimicrobial Drug Resistance and Molecular Characterization of Salmonella Isolated from Domestic Animals, Humans and Meat Products

A Study on Class I Integrons and Antimicrobial Resistance among Clinical Staphylococci Isolates from a Turkish Hospital

Antibiotic Resistance, Plasmids and Integron Profile of Salmonella Species Isolated from Poultry Farm and Patients

Contact Info

Product

Resources

About