Identification and Characterization of Fibronectin-Binding Peptides in Gelatin

Liu, Yuying; Gao, Jianping; Liu, Lin; Kang, Jiyao; Luo, Xi; Kong, Yuanyuan; Zhang, Guifeng

doi:10.3390/polym14183757

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…To mimic the microenvironment in vitro many studies have been conducted mixing the ECMs with various cell types. Binding between collagen and fibronectin could cause conformation changes in fibronectin and could result in a biological response such as cell attachment, migration, and wound repair [ 28 ]. In a C2C12 myoblast, a fibronectin-collagen cell showed a significant increase in differentiation [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

The fibronectin concentration that optimally maintains porcine satellite cells

Han¹,

Jang²,

Kim³

et al. 2023

Anim Biosci

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Objective: ‘Cultured meat’ has been suggested as means of solving the problems associated with overpopulation and gas emissions. Satellite cells are a major component in the production of cultured meat; however, these cells cannot be maintained <i>in vitro</i> over long periods. Fibronectin is a glycoprotein that affects biological processes such as cell adhesion, differentiation, and migration. Unfortunately, the characteristics of porcine satellite cells grown in a long-term culture when exposed to fibronectin-coated dishes are unknown. The objective of this study was to investigate the appropriate concentration of fibronectin coated dishes for proliferation and maintenance of porcine satellite cells at long-term culture.Methods: In this study, we isolated the satellite cells and fibroblast cells with pre-plating method. We next analyzed the cell doubling time, cell cycle, and rate of expressed paired box 7 (Pax7) and myogenic differentiation 1 (MyoD1) in porcine satellite cells cultured with 20 μg/mL of fibronectin-, gelatin-, and non-coated dishes at early and late passage. We then analyzed the proliferation of porcine satellite cells with various concentrations of mixed gelatin/fibronectin. We next determined the optimal concentration of fibronectin that would encourage proliferation and maintenance of porcine satellite cells in a long-term culture.Results: Doubling time was lowest when 20 μg/mL of fibronectin was used (as tested during an early and late passage). Levels of expressed Pax7 and MyoD1, assessed using immunocytochemistry, were highest in cells grown using fibronectin-coated dishes. The proliferation of gelatin/fibronectin mixed coatings had no significant effect on porcine satellite cells. The concentration of 5 μg/mL fibronectin coated dishes showed the lowest doubling time and maintained expression of Pax7.Conclusion: Fibronectin with 5μg/mL effectively maintains porcine satellite cells, a discovery that will be of interest to those developing the next generation of artificial meats.

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Section: Discussionmentioning

confidence: 99%

The fibronectin concentration that optimally maintains porcine satellite cells

Han¹,

Jang²,

Kim³

et al. 2023

Anim Biosci

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“…The research of Liu and Li shows that the spatial inhomogeneous structure also has the ability to improve energy absorption efficiency. [31] Inspired by these studies, the design objects of our method are not limited to periodic structures, and more structural designs based on the stressstrain curve are expected to be obtained by multiscale or gradient optimizations. The proposed method also has broad applicational prospects in dealing with material nonlinearity and geometric nonlinearity, and more meaningful work is worth exploring.…”

Section: Inverse Design Of Multiplatform Energy-absorbing Structure F...mentioning

confidence: 99%

Inverse Design of Energy‐Absorbing Metamaterials by Topology Optimization

et al. 2022

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Compared with the forward design method through the control of geometric parameters and material types, the inverse design method based on the target stress‐strain curve is helpful for the discovery of new structures. This study proposes an optimization strategy for mechanical metamaterials based on a genetic algorithm and establishes a topology optimization method for energy‐absorbing structures with the desired stress‐strain curves. A series of structural mutation algorithms and design‐domain‐independent mesh generation method are developed to improve the efficiency of finite element analysis and optimization iteration. The algorithm realizes the design of ideal energy‐absorbing structures, which are verified by additive manufacturing and experimental characterization. The error between the stress‐strain curve of the designed structure and the target curve is less than 5%, and the densification strain reaches 0.6. Furthermore, special attention is paid to passive pedestrian protection and occupant protection, and a reasonable solution is given through the design of a multiplatform energy‐absorbing structure. The proposed topology optimization framework provides a new solution path for the elastic‐plastic large deformation problem that is unable to be resolved by using classical gradient algorithms or genetic algorithms, and simplifies the design process of energy‐absorbing mechanical metamaterials.

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Identification and Characterization of Fibronectin-Binding Peptides in Gelatin

Cited by 2 publications

References 24 publications

The fibronectin concentration that optimally maintains porcine satellite cells

The fibronectin concentration that optimally maintains porcine satellite cells

Inverse Design of Energy‐Absorbing Metamaterials by Topology Optimization

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