Identification and characterization of four distinct asparagine synthetase (AsnS) genes in maize (Zea mays L.)

Todd, James F.; Screen, Steven E.; Crowley, James H.; Peng, Jiexin; Andersen, Scott; Brown, Todd J.; Qi, Qungang; Fabbri, Brad; Duff, Stephen M. G.

doi:10.1016/j.plantsci.2008.08.004

Cited by 33 publications

(50 citation statements)

References 42 publications

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“…In a previous study we identified four AsnS genes in maize and showed that they have very different expression profiles [2]. This suggests that these AsnS genes play different physiological roles during plant growth and development and it is possible that the enzymes may have different physical and kinetic properties which reflect these different functions.…”

Section: Introductionmentioning

confidence: 98%

“…Asn is a major amino acid in maize and since AsnS is the primary means of Asn synthesis in plants it plays a very important role in nitrogen metabolism [1,2]. All plant forms of AsnS studied to date have shown a very strong preference for glutamine as an amino donor and are phylogenetically and kinetically much more closely related to Escherichia coli AsnB (E. coli asparagine synthetase type B; glutamine-preferred) rather than E. coli AsnA (E. coli asparagine synthetase type A; NH 4 þ -dependent) [2]. In plants, glutamine-preferred AsnS is thought to be the major route of asparagine biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

“…Multiple AsnS genes have been found in pea [6], sunflower [7], soybean [8], barley [9], Arabidopsis [10,11], and corn [2].…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study four unique AsnS genes from Zea mays were identified, cloned and expressed in E. coli cells [2]. We have now cloned and expressed a soy AsnS gene [8] in E. coli.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A kinetic comparison of asparagine synthetase isozymes from higher plants

Duff

Reich

et al. 2011

Plant Physiology and Biochemistry

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

“…Multiple AsnS genes have been found in pea [6], sunflower [7], soybean [8], barley [9], Arabidopsis [10,11], and corn [2].…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study four unique AsnS genes from Zea mays were identified, cloned and expressed in E. coli cells [2]. We have now cloned and expressed a soy AsnS gene [8] in E. coli.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A kinetic comparison of asparagine synthetase isozymes from higher plants

Duff

Reich

et al. 2011

Plant Physiology and Biochemistry

Self Cite

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“…In addition, Asn also accumulates under stress conditions , and it is proposed that this biological response would help maintain the cell osmotic pressure (Gilbert et al 1998;Garthwaite et al 2005). Several genes encoding AS have been identified, whose number can vary between species Todd et al 2008). Although there are variations in the regulatory mechanisms of these genes, in general one of the AS genes is induced by the decrease in soluble carbohydrate levels and in some cases by dark, while the expression of the other AS gene is stimulated by light and by the presence of carbohydrates .…”

Section: Introductionmentioning

confidence: 99%

Boron deficiency increases expressions of asparagine synthetase, glutamate dehydrogenase and glutamine synthetase genes in tobacco roots irrespective of the nitrogen source

Beato

Rexach

Navarro-Gochicoa

et al. 2014

Soil Science and Plant Nutrition

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Nitrate-ammonium (N-A) and ammonium-nitrate (A-N) transition experiments were performed with tobacco (Nicotiana tabacum, cv Gatersleben) plants to study whether the increased expression of ammonium assimilation-related genes found under short-term boron (B) deficiency is maintained when the nitrogen source changes. Asparagine synthetase (AS), glutamate dehydrogenase (GDH) and glutamine synthetase (GS) gene overexpressions in roots under B deprivation were detected in both nitrogen transitions, although GS and, especially, AS up-regulations were observed earlier in the ammonium-nitrate transition. Transcript levels were inversely correlated with hexose contents after 24 h of B deprivation. Our results suggest that tobacco roots respond to short-term B deficiency by increasing the expression of AS, GDH and GS genes irrespectively of the nitrogen source. A hypothetical model to explain these results is proposed and discussed. Thus, the combined action of these enzymes would avoid the accumulation of ammonia and help maintain the activity of the tricarboxylic acid cycle when tobacco roots are affected by B deficiency, the plant organ that first senses this stress. Therefore, under B deficiency, they would act as an ammonium detoxifying mechanism, being a complement to the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway. We propose that the protective role of these enzymes could be extended to changing nitrogen-supply conditions.

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Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress

Curtis

Bò

Tucker

et al. 2018

Food and Energy Security

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A detailed network describing asparagine metabolism in plants was constructed using published data from Arabidopsis (Arabidopsis thaliana) maize (Zea mays), wheat (Triticum aestivum), pea (Pisum sativum), soybean (Glycine max), lupin (Lupus albus), and other species, including animals. Asparagine synthesis and degradation is a major part of amino acid and nitrogen metabolism in plants. The complexity of its metabolism, including limiting and regulatory factors, was represented in a logical sequence in a pathway diagram built using yED graph editor software. The network was used with a Unique Network Identification Pipeline in the analysis of data from 18 publicly available transcriptomic data studies. This identified links between genes involved in asparagine metabolism in wheat roots under drought stress, wheat leaves under drought stress, and wheat leaves under conditions of sulfur and nitrogen deficiency. The network represents a powerful aid for interpreting the interactions not only between the genes in the pathway but also among enzymes, metabolites and smaller molecules. It provides a concise, clear understanding of the complexity of asparagine metabolism that could aid the interpretation of data relating to wider amino acid metabolism and other metabolic processes.

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Identification and characterization of four distinct asparagine synthetase (AsnS) genes in maize (Zea mays L.)

Cited by 33 publications

References 42 publications

A kinetic comparison of asparagine synthetase isozymes from higher plants

A kinetic comparison of asparagine synthetase isozymes from higher plants

Boron deficiency increases expressions of asparagine synthetase, glutamate dehydrogenase and glutamine synthetase genes in tobacco roots irrespective of the nitrogen source

Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress

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