Identification and characterization of fungi pathogen causing fruit rot disease of watermelon (<i>Citrullus lanatus</i>)

Oyedeji, E. O.; Arogundade, Olawale; Tairu, Folasade; Elum, C. G.

doi:10.1080/03235408.2021.2019990

Cited by 4 publications

(6 citation statements)

References 14 publications

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“…Fusarium is considered to be one of the most important genera of plant pathogens, as it is known to cause serious diseases in several economic plants—including watermelons—grown around the world [ 16 , 60 , 61 ]. Traditional approaches to the characterization and identification of the Fusarium species are mainly based on morphological characteristics [ 38 , 58 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

“…Our results are supported by previous studies that reported that Fusarium species are the cause of various disease symptoms in watermelons in tropical and subtropical regions around the world [ 73 , 74 , 75 , 76 ]. Prior to this study, F. solani , F. oxysporum , F. verticillioides , and F. chlamydosporum were considered to be the causal agents of fruit rot in watermelons in Nigeria [ 16 , 24 , 77 ]. In particular, F. equiseti was found to cause fruit rot in watermelons cultivated in China [ 78 ], Malaysia [ 26 ], and the United States [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

“…The damage to watermelons caused by fruit rot diseases can result in significant losses for farms in terms of productivity and quality [ 14 ]. Watermelons can be affected by a variety of diseases caused by bacteria, fungi, and viruses throughout the growing season, harvest procedure, and postharvest storage period [ 13 , 15 , 16 ]. Fruit rot disease is known to be the most common and widespread disease in watermelon fruits during the pre and postharvest periods (e.g., storage and transportation) [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Nuangmek

Kumla

Khuna

et al. 2023

Plants

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Fruit rot caused by phytopathogenic fungi is one of the major diseases affecting watermelons (Citrullus lanatus) around the world, which can result in unmarketable fruits and significant economic losses. Fruit rot was observed on watermelons throughout the postharvest storage periods in Phayao Province, northern Thailand in 2022. For the present study, a total of ten fungal isolates were isolated from the rot lesions of watermelons. All obtained fungal isolates were then characterized in terms of their pathogenicity. The results indicated that only four fungal isolates caused rot disease with similar symptoms during the postharvest storage period. Based on their morphological characteristics, these four fungal isolates were identified as belonging to the genus Fusarium. Using multi-gene phylogenetic analyses with a combination of the translation elongation factor 1-alpha (tef-1), calmodulin (cam), and RNA polymerase second largest subunit (rpb2) genes, the fungal isolates were subsequently identified as Fusarium compactum and F. paranaense. Taken together, the results of this study indicate that F. compactum and F. paranaense cause fruit rot disease in watermelons. To the best of our knowledge, this is the first study to report F. compactum and F. paranaense as novel pathogens of watermelon fruit rot both in Thailand and elsewhere in the world.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Nuangmek

Kumla

Khuna

et al. 2023

Plants

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“…Fusarium is considered to be one of the most important genera of plant pathogens, as it is known to cause serious diseases in several economical plants, including cucumbers, watermelons, tomatoes, etc. (Alnuaimy et al, 2017;Oyedeji et al, 2022;Ajmal et al, 2023). Cucumber Fusarium wilt disease caused a significant problem in cucumber production.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterizations of a few species of Fusarium infecting cucumber in greenhouse conditions

Hussein,

Al-Janabi,

Al-Janabi

et al. 2024

JANS

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One of the most problematic and devastating diseases affecting cucumber production is Fusarium wilt (Cucumis sativus L.). The present study aimed to characterize Fusarium species isolated from three different regions of cucumber fields in Babylon province, Iraq and identify them based on morphological features and phylogenetic analyses. This study showed the presence of Fusarium incarnatum, F. solani and F. oxysporum in cucumber roots. Fusarium species were phylogenetically analyzed based on internal transcribed spacer (ITS) regions. All Fusarium isolates were pathogenic to cucumber cultivars but varied significantly in their growth and pathogenicity toward cucumber seeds during the experiment. F. solani-2 was greater in growth and pathogenicity than all other Fusarium species. In contrast, F. solani-1 was the lowest. The data obtained from morphological and molecular studies sufficiently supported each other, and the phylogenetic trees based on ITS were distinguished. Closely related species and distinctly separated all morphological taxa. These findings are reported for the first time for the cucumber plant in Babylon province. All these ITS sequences showed homologous to those of Fusarium species isolates in the GenBank database with a similarity percentage of 99%. To the best of present knowledge, this is the first molecular record of F. incarnatum on the cucumber plants in Iraq. The study concluded that F. incarnatum was reported for the first time in Iraq and worldwide as a causal agent of wilt disease in cucumber plants. The three species of Fusarium have different pathogenic abilities, highlighting their disease incidence, growth, and pathogenicity.

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“…Fruit rot disease is one of the most typical preharvest and postharvest diseases of cucurbit fruits in Thailand and worldwide [4,[15][16][17][18][19]. This disease has caused huge losses through reductions in harvest yields and lowered standards of quality, both of which can have a significant negative economic impact [20][21][22]. Previous studies indicate that the fungal species belonging to the genera Alternaria, Didymella, Epicoccum, Fusarium, Lasiodiplodia, Myrothecium, Penicillium, Phomopsis, Phytophthora, Pythium, Rhizoctonia, and Sclerotium have been reported as causal agents of fruit rot in cucurbits (cantaloupes, cucumbers, melons, pumpkins, squashes, and watermelons) [4,15,17,[23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Morphology, Molecular Identification, and Pathogenicity of Two Novel Fusarium Species Associated with Postharvest Fruit Rot of Cucurbits in Northern Thailand

Khuna

Kumla

Thitla

et al. 2022

JoF

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Fruit rot of cucurbits caused by several pathogenic fungi has become an important postharvest disease worldwide. In 2022, fruit rot on watermelon (Citrullus lanatus) and muskmelon (Cucumis melo) was observed during the postharvest storage phase in the Chiang Mai and Phitsanulok Provinces of northern Thailand. These diseases can lead to significant economic losses. This present study was conducted to isolate the causal agent of fungi in lesions of fruit rot. A total of four fungal isolates were obtained, of which two isolates (SDBR-CMU422 and SDBR-CMU423) were obtained from rot lesions of watermelons, while the remaining isolates (SDBR-CMU424 and SDBR-CMU425) were obtained from rot lesions of muskmelons. All fungal isolates were identified using both morphological characteristics and molecular analyses. Morphologically, all isolated fungal isolates were classified into the genus Fusarium. Multi-gene phylogenetic analyses of a combination of the translation elongation factor 1-alpha (tef-1), calmodulin (cam), and RNA polymerase second largest subunit (rpb2) genes reveled that four fungal isolates belonged to the Fusarium incarnatum–equiseti species complex and were distinct from all other known species. Thus, we have described them as two new species, namely F. citrullicola (SDBR-CMU422 and SDBR-CMU423) and F. melonis (SDBR-CMU424 and SDBR-CMU425). A full description, illustrations, and a phylogenetic tree indicating the position of both new species have been provided. Moreover, pathogenicity tests were subsequently performed and the results showed that F. citrullicola and F. melonis caused symptoms of fruit rot on inoculated watermelon and muskmelon fruits, respectively. Notably, this outcome was indicative of the symptoms that appeared during the postharvest storage phase. To our knowledge, two new pathogenic fungi, F. citrullicola and F. melonis, are new causal agents of watermelon and muskmelon fruit rot, respectively. Importantly, these findings provide valuable information for the development of effective strategies for the monitoring and prevention of these diseases.

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Identification and characterization of fungi pathogen causing fruit rot disease of watermelon (Citrullus lanatus)

Cited by 4 publications

References 14 publications

Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Identification and characterizations of a few species of Fusarium infecting cucumber in greenhouse conditions

Morphology, Molecular Identification, and Pathogenicity of Two Novel Fusarium Species Associated with Postharvest Fruit Rot of Cucurbits in Northern Thailand

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