Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India

Singha, Irom Manoj; Kakoty, Yelena; Unni, B. G.; Das, Jayshree; Kalita, Mohan Chandra

doi:10.1016/j.jgeb.2016.07.001

Cited by 55 publications

(42 citation statements)

References 29 publications

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“…Then, in order to give more resolution to this complex and differentiate EA-FP0013 from the other species, two other molecular markers were selected, EF and Btub. These markers have been used for many researchers around the world with successful results [23], [24], [25]. A phylogenetic analysis was carried out with concatenated genes and, through the ML and BI methodologies, a single phylogenetic tree was obtained (Figure 4).…”

Section: Gene Sequence Analysis and Molecular Phylogeneticsmentioning

confidence: 99%

Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia

et al. 2018

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The cultivation of sugarcane represents an important part in the Colombian economy due to the diverse applications in industries like liquor, food, paper and biofuels. Sugarcane worldwide production is affected by the presence of phytopathogenic agents, mainly filamentous fungi such as Physalospora tucumanensis (red rot disease) and Fusarium spp. To date in Colombia, Pokka boheng disease whose causal agent is the fungus Fusarium verticillioides, has not been reported, which is why it is necessary to identify appropriately this microorganism, responsible for losses in productivity and food contamination. In order to isolate and identify the infectious agent from symptomatic tissues, disinfection and culture in liquid and solid culture mediums were performed in malt extract (2%) and yeast extract (0.2%) both liquid and solid, for 7 days. After several replicates in agar plate, a purification was made along with a morphological characterization based on the shape and color of the mycelium, as well as the type of spores generated. Additionally, the genetic material was extracted and gene markers (ITS, Elongation Factor 1-α (EF) and β-Tubulin (Btub)) were amplified by PCR. Then, DNA sequencing was used to obtain the data to make a phylogenetic reconstruction by probabilistic methods (Maximum Likelihood and Bayesian Inference). The isolated strain, named as EA-FP0013 was located in the Fujikuroi complex group, with high probable identity to Fusarium verticillioides. Thus, early and species-specific identification of these fungal isolates by molecular methods may allow the timely diagnosis of emerging pathophysiological diseases of interest in the region, and thus propose the respective control strategies.

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Section: Gene Sequence Analysis and Molecular Phylogeneticsmentioning

confidence: 99%

Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia

et al. 2018

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“…In addition it can be done crop rotation, balanced fertilization, avoid giving high N and low K, and good drainage to prevent attacks. Biological control can also be carried out with Tric hoderma sp antagonists [13].…”

Section: ) Stem Rot (Fusarium Sp)mentioning

confidence: 99%

The Association Rule of Corn Disease Symptoms by Using Frequent Pattern Growth and Random Forest

Yasid¹

2018

Proceedings of the International Conference on Science and Technology (ICST 2018)

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Despite proper soil health management, pests and diseases control is also an important task in corn farming for improving both the quality and the quality of the crops production. One way to address this challenge is to identify the emerging symptoms accurately to help define the appropriate solution. Downy, Leaf Blight, Rot midrib, Rot Stem, Leaf Rust, Burnt, Dwarf, Leaf spot are among the common symptoms on corn crops. Failure to identify these symptoms in the early stage could result adverse effects on the corn crops and reduce the production in the long run. This study therefore aims to classify corn disease symptoms to know the real disease. In this study, Decision trees and Frequent Pattern Growth (FP Growth) are employed. Analysis from the data indicated accuracy values of decision tree J4.5 (ID3) of 95%, J.48 of 96% and Random Forest of 95%. While for the relation pattern with 9 attributes and 2 classes, obtained the most often arise rule is the mycelium symptom or cotton with the leaves color turning red / brown / ash / chlorotic with accuracy of 92%.

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“…Lin et al (2014) used ITS as a molecular marker for identification of the F. verticillioides and F. proliferatum associated with sugarcane infection in Pokkah Boeng, China. Recently, Singha et al (2016) demonstrated that molecular profiling using ITS is an indispensable method for rapid identification of Fusarium sp. isolated from Fusarium-wilt of tomato from Assam, North East India.…”

Section: Upgma Analysismentioning

confidence: 99%

Diversity and identity of Fusarium species occurring on fruits, vegetables and food grains

Ingle

2017

Nusantara Biosci

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Abstract. Ingle AP. 2017. Diversity and identity of Fusarium species occurring on fruits, vegetables and food grains. Nusantara Bioscience 9: 44-51. The members of the genus Fusarium are the most important pathogens associated with different plants including crop plants, fruits, vegetables, etc. Fusarium species are mainly responsible for wilts, blights, root-rots and cankers in these plants. Fusarium spp. produces many types of mycotoxins, which can unfavorably affect human and animal health. In the present study, 48 isolates of Fusarium were recovered from various fruits, vegetables and food grains. Out of these, 23 isolates were recovered from vegetables, 14 from food grains and 11 from fruits. Further, different isolates recovered from infected plant materials were identified on the basis of morphological, cultural and microscopic characteristics.

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Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India

Cited by 55 publications

References 29 publications

Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia

Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia

The Association Rule of Corn Disease Symptoms by Using Frequent Pattern Growth and Random Forest

Diversity and identity of Fusarium species occurring on fruits, vegetables and food grains

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