Identification and characterization of genes related to salt stress tolerance within segregation distortion regions of genetic map in F2 population of upland cotton

Abstract

Segregation distortion (SD) is a genetic mechanism commonly found in segregating or stable populations. The principle behind this puzzles many researchers. The F2 generation developed from wild Gossypium darwinii and G. hirsutum CCRI12 species was used to investigate the possible transcription factors within the segregation distortion regions (SDRs). The 384 out of 2763 markers were distorted in 29 SDRs on 18 chromosomes. Good collinearity was observed among genetic and physical maps of G. hirsutum and G. barbadense syntenic blocks. Total 568 genes were identified from SDRs of 18 chromosomes. Out of these genes, 128 belonged to three top-ranked salt-tolerant gene families. The DUF597 contained 8 uncharacterized genes linked to Pkinase (PF00069) gene family in the phylogenetic tree, while 15 uncharacterized genes clustered with the zinc finger gene family. Two hundred thirty four miRNAs targeted numerous genes, including ghr-miR156, ghr-miR399 and ghr-miR482, while others targeted top-ranked stress-responsive transcription factors. Moreover, these genes were involved in the regulation of numerous stress-responsive cis-regulatory elements. The RNA sequence data of fifteen upregulated genes were verified through the RT-qPCR. The expression profiles of two highly upregulated genes (Gh_D01G2015 and Gh_A01G1773) in salt-tolerant G. darwinii showed antagonistic expression in G. hirsutum. The results indicated that salt-tolerant genes have been possibly transferred from the wild G. darwinii species. A detailed functional analysis of these genes can be carried out which might be helpful in the future for gene cloning, transformation, gene editing and the development of salt-resistant cotton varieties.

Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle

“…Cytokinins (CKs) for example, take part in numerous physiological activities such as cell division and morphogenesis, flower and seed development and chloroplast development [12][13][14][15][16]. CKs can also regulate the abiotic stresses by the up-regulation of nitrogen metabolism and antioxidant defense system of plants [12,[17][18][19][20][21]; the importance of CKs in the regulation of heavy metal stress tolerance in plants is well known [11,17,18,22], but the mechanism of CKs induced regulation of heavy metal stress tolerance needs further investigation. The work reported here aimed to determine the effect of exogenous application of different level of KN (KN L , KN M and KN H ) on Trigonella seedlings (in order to counteract Cd phyto-toxicity) by examining their effects on growth and photochemistry of the PS II and AsA-GSH cycles.…”

Biochar and Arbuscular mycorrhizal fungi mediated enhanced drought tolerance in Okra (Abelmoschus esculentus) plant growth, root morphological traits and physiological properties

“…Drought stress is a major environmental stress, causing reduction of biological function in many crops ( Pereira et al, 2006 , Golldack et al, 2014 , Hussain et al, 2018 , Shehzad et al, 2021 ). Drought stress negatively impacts seed germination ( Kaya et al, 2006 , Farooq et al, 2009 ).…”

Growth attributes, biochemical modulations, antioxidant enzymatic metabolism and yield in Brassica napus varieties for salinity tolerance

“…Such imbalances result in increased production of Reactive Oxygen Species (ROS). In response to ROS production, plant switch on its various defense mechanism like the production of antioxidant both enzymatic and non-enzymatic ( Apel and Hirt, 2004 , Mahmood et al, 2016 , Naveed et al, 2020 , Noman et al, 2018b , Shehzad et al, 2021 ). Salt stress caused enhancement in activities of ROS scavenging enzymes i.e.…”