IDENTIFICATION AND CHARACTERIZATION OF HEAT SHOCK PROTEINS IN FOUR EGYPTIAN MAIZE INBRED LINES (Zea mays L.)

Mahmoud, Fatma E.; Rashed, M. A.; Khalil, Kamal M.; Abou-Deif, M. H.

doi:10.21608/ajs.2018.28384

Cited by 4 publications

(3 citation statements)

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“…Plant materials: Two Egyptian inbred lines of maize (Zea mays L.) K1 and K7 were used in this study. The two lines K1 and K7 were chosen because they showed a high number of HSP bands after exposure to heat stress at 45EC as compared to other maize lines in a previous study 28 and the proteomic analysis for the HSPs manifested five spots were induced after heat treatment at 45EC 29 . Also, high expression levels of HSP genes in these two lines were detected by using western blot and real-time Polymerase Chain Reaction (PCR) methods 30 .…”

Section: Study Areamentioning

confidence: 99%

Cloning, Transformation and Localization of Thermotolerance ZmHSP22 Gene in Maize

Rashed¹,

Abou-Deif²,

Khalil³

et al. 2022

Asian J. of Plant Sciences

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Background and Objective: Plant heat shock proteins play an essential role in environmental stress tolerance. Maize small heat shock proteins were shown to accumulate to high levels during heat stress and play important role in the tolerance mechanism of plants to abiotic stresses. The purposes of the study were cloning, transformation and localization of the ZmHSP22 gene from an Egyptian line of maize (Zea mays L.). Materials and Methods: ZmHSP22 gene has been isolated, cloned and sequenced from the Egyptian maize line K1 after heat treatment at 45EC for 4 hrs. The gene was cloned in plant expression vector pCAMPIA1302 which produced a sub-cellular localization 22 kD protein. Transformation of the gene was performed after fusing with a green fluorescent protein and mitochondrial marker in leaves of the tobacco plant by the agroinfiltration method. Results: The isolated gene ZmHSP22 gave a size of 657 base pair encodes a protein of 218 amino acids. It has been submitted in the GenBank under accession number MT019963. The confocal fluorescence microscopy demonstrated that the gene ZmHSP22 expressed a high level of protein HSP22 accumulated in mitochondria during heat stress in the transgenic tobacco plants. Conclusion: ZmHSP22 gene encodes a mitochondrial protein HSP22 helps in protecting plant mitochondrial proteins against denaturation and degradation. ZmHSP22 gene will help to develop plant varieties adaptable to adverse environmental conditions.

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Section: Study Areamentioning

confidence: 99%

Cloning, Transformation and Localization of Thermotolerance ZmHSP22 Gene in Maize

Rashed¹,

Abou-Deif²,

Khalil³

et al. 2022

Asian J. of Plant Sciences

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“…An Egyptian inbred line of maize (Zea mays L.) "K1" was used in this study which was developed by the plant breeding group, Genetic and Cytology Department, National Research Centre, Giza, Egypt. The inbred "K1" is one of four Egyptian maize inbred lines (Zea mays L.) that were studied to identify heat shock proteins under heat stress at 45°C as protein markers for detecting the thermo-tolerance inbred lines, which showed four bands of heat shock proteins with molecular weights of 82, 22, 17, and 10 kDa after exposing to 45°C for 2 and 4 h which may be an indication of thermo-tolerance (Mahmoud et al 2018).…”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis of heat shock proteins in maize (Zea mays L.)

et al. 2019

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Background: Maize is one of the important cereal food crops in the world. High temperature stress causes adverse influence on plant growth. When plants are exposed to high temperatures, they produce heat shock proteins (HSPs), which may impart a generalized role in tolerance to heat stress. Proteome analysis was performed in plant to assess the changes in protein types and their expression levels under abiotic stress. The purpose of the study is to explore which proteins are involved in the response of the maize plant to heat shock treatment. Results:We investigated the responses of abundant proteins of maize leaves, in an Egyptian inbred line of maize "K1", upon heat stress through two-dimensional electrophoresis (2-DE) on samples of maize leaf proteome. 2-DE technique was used to recognize heat-responsive protein spots using Coomassie Brilliant Blue (CBB) and silver staining. In 2-D analysis of proteins from plants treated at 45°C for 2 h, the results manifested 59 protein spots (4.3%) which were reproducibly detected as new spots where did not present in the control. In 2D for treated plants for 4 h, 104 protein spots (7.7%) were expressed only under heat stress. Quantification of spot intensities derived from heat treatment showed that twenty protein spots revealed clear differences between the control and the two heat treatments. Nine spots appeared with more intensity after heat treatments than the control, while four spots appeared only after heat treatments. Five spots were clearly induced after heat treatment either at 2 h or 4 h and were chosen for more analysis by LC-MSMS. They were identified as ATPase beta subunit, HSP26, HSP16.9, and unknown HSP/Chaperonin. Conclusion:The results revealed that the expressive level of the four heat shock proteins that were detected in this study plays important roles to avoid heat stress in maize plants.

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“…The four lines were previously studied to identify HSPs under HS at 45°C as protein markers for detecting the thermo-tolerance lines. The results indicated that the three lines K1, K7 and G342 are tolerant and the line Rg59 is susceptible (Mahmoud et al, 2018).…”

Section: Plant Materialsmentioning

confidence: 97%

Expression levels of heat shock proteins through western blot and real-time polymerase chain reaction in maize

2021

JJBS

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High temperature stress (HTS) is one of the most detrimental abiotic stresses which adversely affect productivity of maize (Zea mays L.). Plants respond to HTS by regulating expression of many genes including heat shock proteins (HSPs). We carried out expression analysis of six HSP genes by western blot (WB) and real-time polymerase chain reaction (RT-PCR) in four contrasting Egyptian maize lines, i.e. K1, K7, G342 (heat tolerant) and Rg59 (heat susceptible), subjected to HTS at seedling stage. The six-maize specific HSP genes (ZmHSP70, ZmHSP22, ZmHSP17.9, ZmHSP17.6, ZmHSP16.9 and transcription factor ZIP60) exhibited distinctive expression patterns in response to heat stress (HS). Higher upregulation of ZmHSP70, ZmHSP22, ZmHSP17.9 and ZmHSP17.6 were found throughout the stress exposure at 45°C for 2 and 4 hours in the heat tolerant lines as compared to the control (25°C) and susceptible line. Meanwhile, the susceptible line showed expression of many HSP genes in the control, but these genes were down-regulated by HS. The line K1 showed expression level for ZmHSP70, ZmHSP17.9, ZmHSP17.6 and ZmHSP16.9 genes more than the other lines indicating its highly tolerance against HTS. Higher upregulation of these six genes in the heat tolerant lines indicated their possible role in protecting plant from adverse effects of HS.

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IDENTIFICATION AND CHARACTERIZATION OF HEAT SHOCK PROTEINS IN FOUR EGYPTIAN MAIZE INBRED LINES (Zea mays L.)

Cited by 4 publications

References 3 publications

Cloning, Transformation and Localization of Thermotolerance ZmHSP22 Gene in Maize

Cloning, Transformation and Localization of Thermotolerance ZmHSP22 Gene in Maize

Proteomic analysis of heat shock proteins in maize (Zea mays L.)

Expression levels of heat shock proteins through western blot and real-time polymerase chain reaction in maize

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