Identification and characterization of hypothalamic circular RNAs associated with bovine residual feed intake

Жао, Леи; Ding, Yanling; Yang, Chaoyun; Wang, Pengfei; Zhao, Zhiyan; Ma, Ying; Shi, Yiting; Kang, Xiaolong

doi:10.1016/j.gene.2022.147017

Cited by 4 publications

(3 citation statements)

References 58 publications

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“…The results indicated that ncRNA play an important role in rumen growth and development at the transcriptional level. Furthermore, RT-qPCR was performed to confirm the reliability of sequencing data [54]. In order to understand the potential functions of the ncRNAs identified, functional enrichment analyses on the target genes of DE ncRNAs were performed.…”

Section: Discussionmentioning

confidence: 99%

Integration of Non-Coding RNA and mRNA Profiles Reveals the Mechanisms of Rumen Development Induced by Different Types of Diet in Calves

Wang

Fan

et al. 2023

Genes

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Selecting suitable feed types and understanding the gastrointestinal digestive mechanism are helpful for the growth and health of calves in intensive dairy farming. However, the effects on rumen development of changing the molecular genetic basis and the regulatory mechanism by using different feed types are still unclear. Nine 7-day-old Holstein bull calves were randomly divided into GF (concentrate), GFF (alfalfa: oat grass = 3:2) and TMR (concentrate: alfalfa grass: oat grass: water = 0.30:0.12:0.08:0.50) diet experiment groups. Rumen tissue and serum samples were collected for physiological and transcriptomic analysis after 80 days. The results showed that serum α-amylase content and ceruloplasmin activity were significantly higher in the TMR group, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis ncRNAs and mRNAs were significantly enriched in the pathways of rumen epithelial development and stimulated rumen cell growth, including the Hippo signaling pathway, Wnt signaling pathway, thyroid hormone signaling pathway, ECM–receptor interaction and the absorption of protein and fat. The circRNAs/lncRNA-miRNAs-mRNA networks constructed, including novel_circ_0002471, novel_circ_0012104, TCONS_00946152, TCONS_00960915, bta-miR-11975, bta-miR-2890, PADI3 and CLEC6A, participated in metabolic pathways of lipid, immune system, oxidative stress and muscle development. In conclusion, the TMR diet could improve rumen digestive enzyme activities, stimulate rumen nutrient absorption and stimulate the DEGs related to energy homeostasis and microenvironment balance, and is thus better than the GF and GFF diets for promoting rumen growth and development.

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Section: Discussionmentioning

confidence: 99%

Integration of Non-Coding RNA and mRNA Profiles Reveals the Mechanisms of Rumen Development Induced by Different Types of Diet in Calves

Wang

Fan

et al. 2023

Genes

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show abstract

“…The endogenous control for mRNA was GAPDH, and 18S snRNA was used as an exogenous control for miRNA. The RT-qPCR reaction procedure was consistent with the previous method in our laboratory [35].…”

Section: Rnas Isolation Cdna Library and Rt-qpcrmentioning

confidence: 92%

miR-144 regulates bovine skeletal muscle satellite cell proliferation and differentiation by targeting the NACC1 gene

Ding,

Zhang,

Zhou

et al. 2024

Preprint

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miRNAs are encoded by eukaryotic genomes and are characterized by tissue-specific and temporal expression, suggesting that miRNAs play multiple roles in different tissues and developmental periods of a species through a variety of regulatory pathways. miR-144 regulates cell development in other species, but its regulatory mechanism in bovine skeletal muscle satellite cells (BSMSCs) is unknown. So, this experiment was designed to elucidate the function of miR-144 in BSMSCs development. It was found that miR-144 promoted the proliferation of BSMSCs, but it plays an inhibitory role in the differentiation process. After transfection of the miR-144 mimic, 476 differentially expressed genes (DEGs) were detected by RNA-seq, and these DEGs mainly regulate adrenergic, MAPK, and PI3K-AKT signaling pathways. Further studies revealed that bta-miR-144 targets binding to the NACC1 gene; whereas NACC1 regulates BSMSCs in a manner opposite to bta-miR-144. These findings suggest that miR-144 negatively regulates BSMSCs development by targeting the NACC1gene.

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“…Furthermore, the liver circRNAs targeted RTP4 regulating residual feed intake (RFI) in Hu lamb ( Zhang et al, 2022 ). Zhao et al (2023) found that the hypothalamic circRNAs regulated phenotypic variation of RFI via sponging miRNAs and regulatory pathways related to nutrient metabolism in cattle. Despite accumulating evidence for an important role of circRNAs in chicken and feed efficiency in animals were reported, our current understanding of their expression in modulating feed efficiency and the heterosis is lacking.…”

Section: Introductionmentioning

confidence: 99%

Multi-tissue transcriptome profiling linked the association between tissue-specific circRNAs and the heterosis for feed intake and efficiency in chicken

Yuan,

Li,

Sun

et al. 2024

Poultry Science

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Identification and characterization of hypothalamic circular RNAs associated with bovine residual feed intake

Cited by 4 publications

References 58 publications

Integration of Non-Coding RNA and mRNA Profiles Reveals the Mechanisms of Rumen Development Induced by Different Types of Diet in Calves

Integration of Non-Coding RNA and mRNA Profiles Reveals the Mechanisms of Rumen Development Induced by Different Types of Diet in Calves

miR-144 regulates bovine skeletal muscle satellite cell proliferation and differentiation by targeting the NACC1 gene

Multi-tissue transcriptome profiling linked the association between tissue-specific circRNAs and the heterosis for feed intake and efficiency in chicken

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