Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization

Zhong, Xueping; Wang, Dan; Zhang, Yibing; Gui, Jian‐Fang

doi:10.1016/j.cbpb.2008.10.013

Cited by 48 publications

(32 citation statements)

References 62 publications

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“…Zhong et al [73] have identified some hypoxia induced genes, e.g., ERO1-L, p53, CPO, HO-1, MKP2, PFK-2, cystatin B, Glut1, BTG1, TGF-beta1 and PGAM1, in crucian carp blastulae embryonic cells by suppression subtractive hybridization. Liao et al [74] carried out transcriptome analysis for crucian carp and found hypoxia induced genes related to glycolysis/gluconeogenesis.…”

Section: Hypoxia Adaptation Mechanisms In Fishesmentioning

confidence: 99%

The hypoxia signaling pathway and hypoxic adaptation in fishes

Xiao

2015

Sci. China Life Sci.

140

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The hypoxia signaling pathway is an evolutionarily conserved cellular signaling pathway present in animals ranging from Caenorhabditis elegans to mammals. The pathway is crucial for oxygen homeostasis maintenance. Hypoxia-inducible factors (HIF-1α and HIF-2α) are master regulators in the hypoxia signaling pathway. Oxygen concentrations vary a lot in the aquatic environment. To deal with this, fishes have adapted and developed varying strategies for living in hypoxic conditions. Investigations into the strategies and mechanisms of hypoxia adaptation in fishes will allow us to understand fish speciation and breed hypoxia-tolerant fish species/strains. This review summarizes the process of the hypoxia signaling pathway and its regulation, as well as the mechanism of hypoxia adaptation in fishes. Approximately 2.5 billion years ago, photosynthesis led to the accumulation of oxygen to levels that were likely toxic to many obligate anaerobes. However, increased availability of atmospheric O 2 led to the evolution of an extraordinarily efficient system of oxidative phosphorylation. In this system, chemical energy stored in the carbon bonds of organic molecules is transferred to the high-energy phosphate bond in ATP, which is then used to power physicochemical reactions in living cells [1]. Additionally, O 2 serves as the final electron acceptor in oxidative phosphorylation, which is not only required for energy production, but is also the direct substrate of many enzymes. Thus, it is critical for the growth, development, and reproduction of organisms. Consequently, metazoans have evolved complicated systems of cellular metabolism and physiology to maintain oxygen homeostasis and have developed a biochemical response to low oxygen levels [2]. There are a number of oxygen-sensing pathways that promote hypoxia tolerance by activating transcription and inhibiting translation: the energy and nutrient sensor mTOR, the unfolded protein response that activates the endoplasmic stress response, and the nuclear factor (NF)-B transcriptional response [3]. However, hypoxia-inducible factors (HIFs) are recognized as master regulators of the cellular response to hypoxic stress [4,5]. The hypoxia signaling pathway is evolutionarily conserved from Caenorhabditis elegans to human beings and it activates similar or homogenous gene expression, resulting in similar physical and biochemical responses. Compared with the terrestrial environment, oxygen concentrations vary greatly in the aquatic environment [6]. Thus, compared with most birds and mammals, fishes are tolerant of this varying oxygen availability. Natural selection by oxygen concentration has facilitated the evolution of fishes with a range of adaptations to variable oxygen concentration. Even in waters at the same latitude, closely related species or different strains within a species exhibit varied adaptations to oxygen concentration. Additionally, closely related fishes distributed in waters at different latitudes exhibit extensive variation in their tolerance of hypoxia. De...

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Section: Hypoxia Adaptation Mechanisms In Fishesmentioning

confidence: 99%

The hypoxia signaling pathway and hypoxic adaptation in fishes

Xiao

2015

Sci. China Life Sci.

140

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“…Since Carassius species have been found to survive many days or even several months of anoxia at low temperature, Carassius auratus blastulae embryonic cells have been used to screen gene expression changes responding to hypoxia stress by suppression subtractive hybridization (SSH). Through this approach, a total of 211 differentially expressed genes are identified, in which most of them are reported for the first time from hypoxia-treated teleosts [138].…”

Section: Hypoxia Tolerance Trait and Candidate Hypoxia Tolerance-relamentioning

confidence: 99%

Molecular basis and genetic improvement of economically important traits in aquaculture animals

2012

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Aquaculture has been believed to be a major Chinese contribution to the world. In recent 20 years, genome and other genetic technologies have promoted significant advances in basic studies on molecular basis and genetic improvement of aquaculture animals, and complete genomes of some main aquaculture animals have been sequenced or announced to be sequenced since the beginning of this century. Here, we review some significant breakthrough progress of aquaculture genetic improvement technologies including genome technologies, somatic cell nuclear transfer and stem cell technologies, outline the molecular basis of several economically important traits including reproduction, sex, growth, disease resistance, cold tolerance and hypoxia tolerance, and present a series of candidate trait-related genes. Finally, some application cases of genetic improvement are introduced in aquaculture animals, especially in China, and several development trends are highlighted in the near future.

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“…So far, over 100 direct target genes of HIF-1 have been identified (Liu et al, 2012). These include growth factors (vascular endothelium growth factor and erythropoietin), enzymes of the glycolytic chain as well as antioxidant enzymes neutralizing reactive oxygen species to protect tissues from damage (Ratcliffe et al, 1998;Zhong et al, 2009). Nevertheless, even larger number of proteins, including tripeptidyl peptidase I, exhibit altered expression and/or activity levels following hypoxic episodes (Zhong et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Although similar studies are not carried out in nerve cells, the above findings show that alterations in TPPI activity may change the expression of proteins important for triggering cell death upon different stimuli, including oxidative stress. In fact, a recent study provides evidence that TPPI activity is up-regulated in goldfish embryonic cells after 24 h severe hypoxia (Zhong et al, 2009). Therefore, a possible involvement of TPPI in the brain response to hypoxic events deserves to be explored in more details.…”

Section: Brain Regionmentioning

confidence: 98%

“…To date, over 100 direct target genes of HIF-1 are identified (Liu et al, 2012). Recent studies show that large number of proteins, including tripeptidyl peptidase I, demonstrate altered expression and/or activity levels following hypoxic episodes (Zhong et al, 2009). Tpipeptidyl peptidase I (TPPI, EC 3.4.14.9) is a lysosomal protease hydrolyzing tripeptides from the amino-terminus of polypeptides and unfolded proteins.…”

Section: Brain Regionmentioning

confidence: 99%

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