Identification and characterization of <i>Aeromonas hydrophila</i> genes encoding the outer membrane receptor of ferrioxamine B and an AraC-type transcriptional regulator

Funahashi, Tatsuya; Tanabe, T.; Maki, Jun; Miyamoto, Katsushiro; Tsujibo, Hiroshi; Yamamoto, Satoshi

doi:10.1080/09168451.2014.932669

Cited by 7 publications

(7 citation statements)

References 43 publications

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“…The fact that orthologs exist in numerous Enterobacteriaceae hints that FoxR is likely to play a similar regulatory role in other bacteria. FoxR orthologs, designated DesR (regulator of DesA), have been reported in Aeromonas hydrophila and Vibrio vulnificus, two waterdwelling Gram-negative bacteria (Funahashi et al, 2014;Tanabe et al, 2005). DesR activates the transcription of desA (equivalent to foxA in S. Typhimurium, see Figure 1) by binding directly to its regulatory region, a process which is enhanced in the presence of nonphysiological concentrations of DFOB (1 mM), but not ferrioxamine B, the ironbound form of DFOB (Tanabe et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…We have confirmed that Fur represses foxA and foxR expression in S. Typhimurium (Bjarnason et al, 2003;Teixidó et al, 2011;Tsolis et al, 1995) and identified potential Fur-binding sites in the foxR and foxA upstream regulatory regions. In V. vulnificus and A. hydrophila, desR and desA promoters were shown to harbor Furbinding sites using a Fur titration assay (Funahashi et al, 2014;Tanabe et al, 2005). Fur utilizes iron as a corepressor to negatively regulate genes involved in many aspects of iron acquisition when iron is plentiful (Bjarnason et al, 2003;Mey et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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FoxR is an AraC‐like transcriptional regulator of ferrioxamine uptake in Salmonella enterica

Saldaña-Ahuactzi

Knodler

2022

Molecular Microbiology

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Salmonella enterica spp. produce siderophores to bind iron with high affinity and can also use three xenosiderophores secreted by other microorganisms, ferrichrome, coprogen, and ferrioxamine. Here we focused on FoxA, a TonB‐dependent transporter of ferrioxamines. Adjacent to foxA is a gene annotated as a helix‐turn‐helix (HTH) domain‐containing protein, SL0358 (foxR), in the Salmonella enterica serovar Typhimurium SL1344 genome. FoxR shares homology with transcriptional regulators belonging to the AraC/XylS family. foxR is syntenic with foxA in the Enterobacteriaceae family, suggesting their functional relatedness. Both foxA and foxR are repressed by the ferric uptake regulator (Fur) under iron‐rich growth conditions. When iron is scarce, FoxR acts as a transcriptional activator of foxA by directly binding to its upstream regulatory region. A point mutation in the HTH domain of FoxR abolished this binding, as did mutation of a direct repeat motif in the foxA upstream regulatory region. Desferrioxamine (DFOE) enhanced FoxR protein stability and foxA transcription but did not affect the affinity of FoxR binding to the foxA regulatory region. In summary, we have identified FoxR as a new member of the AraC/XylS family that regulates xenosiderophore‐mediated iron uptake by S. Typhimurium and likely other Enterobacteriaceae members.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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FoxR is an AraC‐like transcriptional regulator of ferrioxamine uptake in Salmonella enterica

Saldaña-Ahuactzi

Knodler

2022

Molecular Microbiology

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“…In Neisseria gonorrhoeae , MpeR activates fetA , encoding the receptor of the enterobactin xenosiderophore [ 33 ]. Finally, DesR activates the transcription of desA, coding for the xenosiderophore ferrioxamine B receptor in Vibrio vulnificus and Vibrio furnissi [ 34 , 35 ] and Aeromonas hydrophila [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

IurV, Encoded by ORF VCA0231, Is Involved in the Regulation of Iron Uptake Genes in Vibrio cholerae

Sachman-Ruiz

Ibarra

Santos

et al. 2020

Genes

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The pathogen Vibrio cholerae has multiple iron acquisition systems which allow bacteria to exploit a variety of iron sources across the different environments on which it thrives. The expression of such iron uptake systems is highly regulated, mainly by the master iron homeostasis regulator Fur but also by other mechanisms. Recently, we documented that the expression of many of the iron-responsive genes is also modulated by riboflavin. Among them, the open reading frame VCA0231, repressed both by riboflavin and iron, encodes a putative transcriptional regulator of the AraC/XylS family. Nonetheless, the genes or functions affected by this factor are unknown. In the present study, a series of in silico analyses was performed in order to identify the putative functions associated with the product of VCA0231. The STRING database predicted many iron uptake genes as functional partners for the product of VCA0231. In addition, a genomic neighborhood analysis with the Enzyme Function Initiative tools detected many Pfam families involved in iron homeostasis genetically associated with VCA0231. Moreover, a phylogenetic tree showed that other AraC/XylS members known to regulate siderophore utilization in bacteria clustered together and the product of VCA0231 localized in this cluster. This suggested that the product of VCA0231, here named IurV, is involved in the regulation of iron uptake processes. RNAseq was performed to determine the transcriptional effects of a deletion in VCA0231. A total of 52 genes were overexpressed and 21 genes were downregulated in response to the iurV deletion. Among these, several iron uptake genes and other iron homeostasis-related genes were found. Six gene ontology (GO) functional terms were enriched in the upregulated genes, of which five were related to iron metabolism. The regulatory pattern observed in the transcriptomics of a subset of genes was independently confirmed by quantitative real time PCR analysis. The results indicate that IurV is a novel regulator of the AraC/XylS family involved in the repression of iron uptake genes. Whether this effect is direct or indirect remains to be determined.

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“…Under iron-limiting conditions, Aer. hydrophila can regulate the expression of an outer membrane receptor encoded by the desA gene to acquire iron from the environment (Funahashi et al 2014). Therefore, desA is crucial for the survival and growth of Aer.…”

Section: Introductionmentioning

confidence: 99%

Development of cross-priming amplification assays for rapid and sensitive detection of Aeromonas hydrophila

Shao

Wang

Liu

et al. 2015

Lett Appl Microbiol

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Aeromonas hydrophila is a human pathogen that infects via exposed wounds or ingestion of contaminated water and food. In this study, a CPA-based PCR method was developed for specific, rapid, cost-effective detection of Aer. hydrophila, and the test results could be visualized without opening the reaction tubes. This is the first report on the application of the CPA method for the detection of Aer. hydrophila. This novel method could be practical for use in primary care facilities and smaller clinical laboratories.

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Identification and characterization of Aeromonas hydrophila genes encoding the outer membrane receptor of ferrioxamine B and an AraC-type transcriptional regulator

Abstract: (2014) Identification and characterization of Aeromonas hydrophila genes encoding the outer membrane receptor of ferrioxamine B and an AraC-type transcriptional regulator, Bioscience, Biotechnology, and Biochemistry, 78

Cited by 7 publications

References 43 publications

FoxR is an AraC‐like transcriptional regulator of ferrioxamine uptake in Salmonella enterica

FoxR is an AraC‐like transcriptional regulator of ferrioxamine uptake in Salmonella enterica

IurV, Encoded by ORF VCA0231, Is Involved in the Regulation of Iron Uptake Genes in Vibrio cholerae

Development of cross-priming amplification assays for rapid and sensitive detection of Aeromonas hydrophila

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