Identification and characterization ofGypsophila paniculatacolor morphs in Sleeping Bear Dunes National Lakeshore, MI, USA

Abstract

Background Gypsophila paniculata (baby’s breath) is an invasive species found throughout much of the northwest United States and western Canada. Recently, plants exhibiting a different color morphology were identified within the coastal dunes along eastern Lake Michigan. The common baby’s breath (G. paniculata) typically produces stems that are purple in color (purple morph), while the atypical morph has stems that are green-yellow (green-yellow morph). The purpose of this study was to characterize these newly identified morphs and determine if they are genetically distinct species from the common baby’s breath in order to assess whether alternative management strategies should be employed to control these populations. Methods We sequenced two chloroplast regions, ribulose-bisphosphate carboxylase gene (rbcL), and maturase K (matK), and one nuclear region, internal transcribed spacer 2 (ITS2), from the purple morphs and green-yellow morphs collected from Sleeping Bear Dunes National Lakeshore, MI, USA (SBDNL). Sequences were aligned to reference sequences from other Gypsophila species obtained from the Barcode of Life Database and GenBank databases. We also collected seeds from wild purple morph and wild green-yellow morph plants in SBDNL. We grew the seeds in a common garden setting and characterized the proportion of green-yellow individuals produced from the two color morphs after 5-months of growth. Results Phylogenetic analyses based upon rbcL, matK, and ITS2 regions suggest that the two color morphs are not distinct species and they both belong to G. paniculata. Seeds collected from wild green-yellow morphs produced a significantly higher proportion of green-yellow individuals compared to the number produced by seeds collected from wild purple morphs. However, seeds collected from both color morphs produced more purple morphs than green-yellow morphs. Discussion Based upon these results, we propose that the two color morphs are variants of G. paniculata. Given the significant difference in the number of green-yellow morphs produced from the seeds of each morph type, we also suggest that this color difference has some genetic basis. We propose that current management continue to treat the two color morphs in a similar manner in terms of removal to prevent the further spread of this species.

Repeat photography of Lake Michigan coastal dunes: Expansion of vegetation since 1900 and possible drivers

“…By their nature, dunes are disturbed landscapes and, as such, can be susceptible to invasive species colonization. G. paniculata, with its deep taproot and large seed disbursement, has successfully directly competed for limited resources from more sensitive native species such as Pitcher's thistle (Cirsium pitcher) (Leimbach-Maus et al, 2020;Yang et al, 2019). Grass planting programs, such as those around Ottawa Beach in the 1980s, have accomplished much the same effect, as one of our repeat photo pairs from that area demonstrates.…”