Identification and characterization of <i>Neopestalotiopsis</i> fungi associated with a novel leaf fall disease of rubber trees (<i>Hevea brasiliensis</i>) in Thailand

Pornsuriya, Chaninun; Chairin, Thanunchanok; Thaochan, Narit; Sunpapao, Anurag

doi:10.1111/jph.12906

Cited by 53 publications

(38 citation statements)

References 34 publications

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“…There were three groups that commonly be studied as Pestalotiopsis, Pseudopestalotiopsis, and Neopestalotiopsis. Neopestalotiopsis can be differed from Pestalotiopsis and Pseudopestalotiopsis from the versicolourous character of media cells [18]. To ensure the species of isolate X1 needed advanced analyses using molecular study.…”

Section: Resultsmentioning

confidence: 99%

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Neopestalotiopsis zimbabwana Isolated from Xylaria Stromata

Hermawan¹,

Safitri²,

Sidiq³

2021

Biotropika

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Neopestalotiopsis is one of pestalotioid fungi. Neopestalotiopsis has versicolourous in the third cell (median cell). This genus is identified as pathogenic and endophytic fungi in the plant. Neopestalotiopsis strain X1 was isolated from Xylaria stromata assumed as endophytic fungi. The surface sterilization was conducted, then isolated into PDA (1/2 receipt of potato dextrose). The morphology was observed in a fresh PDA medium. Genomic DNA was extracted and amplified for the Large Subunit region. A phylogenetic tree was built using RAxML Black Box. The morphology showed that the five cells in a conidium were an appearance with the black color of the septate in the third cell (median cell). The appendage on the basal cell was only a single tubular appendage. The appendage on the apical was two until three tubular appendages. The phylogenetic tree showed that Neopestalotiopsis strain X1 was Neopestalotiopsis zimbabwana. The sequence was deposited into GenBank as MW422813. This study was the first report for Neopestalotiopsis species isolated from micro-mushroom (Xylaria stromata).

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Section: Resultsmentioning

confidence: 99%

“…Neopestalotiopsis species were known as endophytic, parasitic, and saprobic fungi [2,10]. Currently, some of them were most as parasitic [22,9,18]. As an endophytic and parasitic fungus, Neopestalotiopsis had been reported that the host was the plant, not yet was reported from the mushroom.…”

Section: Resultsmentioning

confidence: 99%

Neopestalotiopsis zimbabwana Isolated from Xylaria Stromata

Hermawan¹,

Safitri²,

Sidiq³

2021

Biotropika

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“…Plant pathogens recently causing emerging diseases in Thailand were obtained from Culture Collection of Pest Management, Faculty of Natural Resources, Prince of Songkla University; details are provided in Table 1 . Airborne pathogens were cultured on PDA for three days before being subjected to bioassay: Corynespora cassiicola , causing leaf spots on lettuce [ 25 ]; Neopestalotiopsis clavispora , causing flower blight on anthurium [ 26 ]; and Neopestalotiopsis cubana , causing leaf fall in rubber trees [ 27 ]. The soilborne pathogen was Sclerotium rolfsii , causing stem rot in Jerusalem artichoke.…”

Section: Methodsmentioning

confidence: 99%

Volatile Organic Compound from Trichoderma asperelloides TSU1: Impact on Plant Pathogenic Fungi

Ruangwong

Wonglom

Suwannarach

et al. 2021

JoF

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Soil microorganisms are well studied for their beneficial effects on plant growth and their impact on biocontrol agents. The production of volatile antifungal compounds emitted from soil fungi is considered to be an effective ability that can be applied in biofumigants in the control of plant diseases. A soil fungus, Trichoderma asperelloides TSU1, was isolated from flamingo flower cultivated soil and identified on the basis of the morphology and molecular analysis of the internal transcribed spacer (ITS), rpb2, and tef1-α genes. To test T. asperelloides TSU1-produced volatile organic compounds (VOCs) with antifungal activity, the sealed plate method was used. The VOCs of T. asperelloides TSU1 inhibited the mycelial growth of fungal pathogens that were recently reported as emerging diseases in Thailand, namely, Corynespora cassiicola, Fusarium incarnatum, Neopestalotiopsis clavispora, N. cubana, and Sclerotium rolfsii, with a percentage inhibition range of 38.88–68.33%. Solid-phase microextraction (SPME) was applied to trap VOCs from T. asperelloides TSU1 and tentatively identify them through gas chromatography–mass spectrometry (GC/MS). A total of 17 compounds were detected in the VOCs of T. asperelloides TSU1, and the dominant compounds were identified as fluoro(trinitro)methane (18.192% peak area) and 2-phenylethanol (9.803% peak area). Interestingly, the commercial 2-phenyethanol showed antifungal activity against fungal pathogens that were similar to the VOCs of T. asperelloides TSU1 by bioassay. On the basis of our study’s results, T. asperelloides TSU1 isolated from soil displayed antifungal abilities via the production of VOCs responsible for restricting pathogen growth.

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“…A total of ten symptomatic stem canker samples of H. polyrhizus were collected from a dragon fruit plantation field in Phatthalung Province, southern Thailand (7 • 45 24.2 N, 99 • 58 47.2 E), kept in a plastic bag in an ice box and taken to a plant pathology laboratory, where isolation was subsequently conducted. The isolation of fungal pathogens was conducted by tissue transplantation according to the method of Pornsuriya et al [14]. Small pieces (2-3 mm) of infected tissue were surface disinfected with 0.5% sodium hypochlorite (NaOCl) [13], rinsed three times with sterilized distilled water (DW), air-dried, placed on water agar (WA), and incubated for 24 h at 28 ± 2 • C. Hyphal tips were cut and transferred to potato dextrose agar (PDA), incubated at ambient temperature (28 ± 2 • C) with natural light-light cycle and subsequently used for further methods.…”

Section: Sample Collection and Pathogen Isolationmentioning

confidence: 99%

“…Thailand is located in tropical and subtropical regions where the weather favors disease spread [8,9]. Several emerging diseases have been isolated and reported to cause diseases in several plant species in this area in the past 5 years [10][11][12][13][14][15][16][17]. However, the identification of fungal pathogens causing diseases on H. polyrhizus in Thailand is rarely documented.…”

Section: Introductionmentioning

confidence: 99%

Morphological, Molecular Identification and Pathogenicity of Neoscytalidium dimidiatum Causing Stem Canker of Hylocereus polyrhizus in Southern Thailand

Wonglom

Pornsuriya

et al. 2022

Plants

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Red-fleshed dragon fruit (Hylocereus polyrhizus) is commonly cultivated in Thailand, especially in southern Thailand, where the weather favors plant growth and development. In 2021, stem canker of H. polyrhizus was observed in a dragon fruit plantation field in Phatthalung Province, southern Thailand. Small, orange circular spots developed on the stem of H. polyrhizus, which later became gray, and the lesion expanded with a mass of conidia. Scytalidium-like fungus was isolated from infected tissues. Based on morphology and phylogenetic analyses of internal transcribed spacer (ITS), nuclear large subunit (LSU) and β-tubulin (tub) sequences of fungal isolates, the fungus was identified as Neoscytalidium dimidiatum. Pathogenicity tests revealed that this isolate caused stem canker on the stem of H. polyrhizus, similar to that observed in the field. Knowledge of the diagnosis of plant diseases is an important step for managing plant diseases and therefore, this finding provides basic information for the development of appropriate strategies to manage stem canker disease on H. polyrhizus plants.

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Identification and characterization of Neopestalotiopsis fungi associated with a novel leaf fall disease of rubber trees (Hevea brasiliensis) in Thailand

Cited by 53 publications

References 34 publications

Neopestalotiopsis zimbabwana Isolated from Xylaria Stromata

Neopestalotiopsis zimbabwana Isolated from Xylaria Stromata

Volatile Organic Compound from Trichoderma asperelloides TSU1: Impact on Plant Pathogenic Fungi

Morphological, Molecular Identification and Pathogenicity of Neoscytalidium dimidiatum Causing Stem Canker of Hylocereus polyrhizus in Southern Thailand

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