Identification and characterization of <i>Trpm4</i> gene involved in regulating Japanese flounder (<i>Paralichthys olivaceus</i>) inflammatory response

Li, Shuo; Feng, Yifei; Zhang, Tongtong; Wang, Shan; Sun, Jinsheng

doi:10.1111/jfd.13493

Cited by 3 publications

(1 citation statement)

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“…In mammals, TRPM4 gating appears to be modulated by heat, but data are limited to a single study ( Talavera et al 2005 ). In teleosts, TRPM4 is associated with learning in response to light stimuli and inflammation, but is otherwise uncharacterized ( You et al 2020 ; Li et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Evolution of Transient Receptor Potential (TRP) Ion Channels in Antarctic Fishes (Cryonotothenioidea) and Identification of Putative Thermosensors

York

Zakon

2022

Genome Biology and Evolution

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Animals rely on their sensory systems to inform them of ecologically relevant environmental variation. In the Southern Ocean, the thermal environment has remained between -1.9 and 5 °C for 15 million years, yet we have no knowledge of how an Antarctic marine organism might sense their thermal habitat as we have yet to discover a thermosensitive ion channel that gates (opens/closes) below 10 °C. Here, we investigate the evolutionary dynamics of transient receptor potential (TRP) channels, which are the primary thermosensors in animals, within cryonotothenioid fishes—the dominant fish fauna of the Southern Ocean. We found cryonotothenioids have a similar complement of TRP channels as other teleosts (∼28 genes). Previous work has shown that thermosensitive-gating in a given channel is species specific, and multiple channels act together to sense the thermal environment. Therefore, we combined evidence of changes in selective pressure, gene gain/loss dynamics, and the first sensory ganglion transcriptome in this clade to identify the best candidate TRP channels that might have a functional dynamic range relevant for frigid Antarctic temperatures. We concluded that TRPV1a, TRPA1b, and TRPM4 are the likeliest putative thermosensors, and found evidence of diversifying selection at sites across these proteins. We also put forward hypotheses for molecular mechanisms of other cryonotothenioid adaptations, such as reduced skeletal calcium deposition, sensing oxidative stress, and unusual magnesium homeostasis. By completing a comprehensive and unbiased survey of these genes, we lay the groundwork for functional characterization and answering long-standing thermodynamic questions of thermosensitive gating and protein adaptation to low temperatures.

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Section: Discussionmentioning

confidence: 99%