Identification and Characterization of IncA/C Conjugative,
            <i>bla</i>
            <sub>NDM-1</sub>
            -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Zheng, Zhiwei; Li, Ruichao; Ye, Lianwei; Chan, Edward Wai-Chi; Chen, Sheng

doi:10.1128/aac.01897-18

Cited by 19 publications

(14 citation statements)

References 10 publications

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“…The extensive β‐lactam resistance profile of C1796, which featured high‐level carbapenem MICs, is consistent with the observation that carbapenemase was produced by this strain in ethylenediaminetetraacetic acid (EDTA)–imipenem microbiological assay (EIM), which was performed as previously described 8,9. Our data showed that cellular extracts of Vb1796, C1796, and a control V. parahemolyticus strain Vb1394 which produced NDM‐1 carbapenemase,10 enabled indicator E. coli strain ATCC25922 to grow in the presence of IMP, whereas extract obtained from a carbapenemase‐negative E. coli strain did not support growth of the indicator organism. This finding suggested that both Vb1796 and C1796 produced carbapenemase, with slightly lower activity being observed in Vb1796, implying that the level of production of carbapenemase could be lower than in the E. coli background.…”

Section: Resultssupporting

confidence: 88%

Genetic and Biochemical Characterization of VMB‐1, a Novel Metallo‐β‐Lactamase Encoded by a Conjugative, Broad‐Host Range IncC Plasmid from Vibrio spp

et al. 2020

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The increasing incidence of phenotypic resistance to carbapenems in recent years is mainly attributed to acquisition of mobile carbapenemase‐encoding genetic elements by major bacterial pathogens. Here, a novel carbapenemase known as Vibrio metallo‐β‐lactamase 1 (VMB‐1), which is encoded by a gene (blaVMB‐1) located in an integron‐bearing, highly transmissible IncC type plasmid, namely pVB1796, is identified and characterized, both genetically and functionally. Recovered from a foodborne Vibrio alginolyticus strain that exhibits resistance to all known β‐lactam antibiotics, pVB1796 is found to possess a hybrid backbone that exhibits unique features of both type 1 and type 2 IncC elements. VMB‐1 exhibits 94% sequence homology with several recently reported but poorly characterized metallo‐β‐lactamases (MBLs) produced by the marine organisms Alteromonadaceae, Glaciecola, and Thalassomonas actiniarum. Sequence alignment analysis shows that VMB‐1 shares a structurally identical active site with subclass B1 MBLs. Importantly, pVB1796 is found to be efficiently transferred from Vibrio to other Gram‐negative bacterial pathogens, including Salmonella typhimurium, Klebsiella pneumoniae, and Acinetobacter baumanni, via conjugation. These findings suggest that blaVMB‐1‐bearing plasmids have the potential to be disseminated to other Gram‐negative bacterial pathogens in the near future and render carbapenems useless in treatment of multidrug resistant infections.

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Section: Resultssupporting

confidence: 88%

Genetic and Biochemical Characterization of VMB‐1, a Novel Metallo‐β‐Lactamase Encoded by a Conjugative, Broad‐Host Range IncC Plasmid from Vibrio spp

et al. 2020

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“…42 Overall, the bla NDM-1 gene has been more frequently reported on broad host range IncA/Ctype plasmids, either in clinical or in environmental isolates. 43 In Algeria, the bla NDM-5 gene was carried by an IncX3 conjugative plasmid in E. coli isolates recovered from dairy cows in the area of Bejaia. 16 The only clinical case of NDM-1producing K. pneumoniae (described above) concerned an IncR plasmid with poor transferability.…”

Section: Discussionmentioning

confidence: 99%

Spread of OXA-48 and NDM-1-Producing Klebsiella pneumoniae ST48 and ST101 in Chicken Meat in Western Algeria

Chaalal

Touati

Bakour

et al. 2021

Microbial Drug Resistance

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We investigated the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in chicken meat in Western Algeria in 2017. Results: From February to July 2017, samples of chicken meat from three poultry farms in Western Algeria were screened for the presence of CPE. Strains were characterized with regard to antibiotic resistance, blactamase content, Plasmid-mediated quinolone resistance, sulfonamide resistance genes, clonality (repetitive sequence-based profiles and multilocus sequence typing) and virulence traits. Of 181 samples analyzed, 29 (16.0%) carbapenemase-producing Klebsiella pneumoniae were detected. Twenty-three OXA-48-producers (79.3%) and six (20.7%) New Delhi metallo (NDM)-1-producers were observed. Clonality analysis showed three distinct lineages and clonal expansions of the OXA-48-producing K. pneumoniae ST48 and the NDM-1producing K. pneumoniae ST101. These isolates harbored fimH, ureA, mrkD, entB, uge, and wabG. Neither capsular serotype genes nor hypermucoviscous phenotype were detected. Plasmid analysis confirmed that all these isolates harbored the transferable IncL and IncFIIK plasmids. Conclusions: This study reports the spread of OXA-48 and NDM-1-producing K. pneumoniae ST48 and ST101 in chicken meat in Western Algeria and demonstrates that food represents a reservoir of the carbapenemases encoding genes.

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“…Thus, it would be possible for the complex integron to expand structure. Surprisingly, the overall form of the complex class 1 integron in pGD31-NDM is identical to that of pC1394 in Vibrio alginolyticus strain Vb1394 isolated from shrimp (Zheng Z. et al, 2018), except for the structure of the class 1 integron, the truncation of IS Aba125 , and the existence of aphA6 (Figure 2). Two copies of IS 5075 are adjacent to the boundaries of the complex integron, leading to putative genesis of an IS 5075 -mediated composite transposon, which may move the complex integron via transposition or homologous recombination.…”

Section: Resultsmentioning

confidence: 91%

Complex Class 1 Integron in a Clinical Escherichia coli Strain From Vietnam Carrying Both mcr-1 and blaNDM–1

Le-Vo

Tran

et al. 2019

Front. Microbiol.

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The co-production of MCR and carbapenemase in Enterobacteriaceae has been previously reported. Here, we describe a clinical strain of Escherichia coli from Vietnam carrying both mcr-1 and blaNDM–1. Whole-genome sequencing showed that the genome of this strain consists of a 4,975,832-bp chromosome and four plasmids. The mcr-1 and blaNDM–1 genes are located on IncI2 and IncA/C2-type plasmids, respectively. Genetic analysis revealed the presence of a multidrug-resistant region with the structure of a novel complex class 1 integron including a class 1 integron region bearing two 5′ conserved segments and one 3′ conserved segment and two complete structures of ISCR1. The complex integron contains aminoglycoside resistance genes aadA2, aadB, strA, strB, and aphA6, quinolone resistance gene qnrA1, extended-spectrum β-lactamase gene blaOXA–4, and a Tn125-like transposon bearing blaNDM–1. In addition, the dfrA12-gcuF-aadA2-cmlA1-aadA1-qacH gene cassette array belonging to the sul3-type integron was also identified, but the region found downstream of the gene cassette array is the IS440-tet(M)-IS26 element instead of the sul3 gene. The results further support that Enterobacteriaceae isolates co-harboring mcr and blaNDM are widely being distributed. The structural characteristics of the complex integron reveal that ISCR1 elements play an important role in the mobilization of blaNDM–1 and the development of multidrug-resistant regions.

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Identification and Characterization of IncA/C Conjugative, bla _NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Cited by 19 publications

References 10 publications

Genetic and Biochemical Characterization of VMB‐1, a Novel Metallo‐β‐Lactamase Encoded by a Conjugative, Broad‐Host Range IncC Plasmid from Vibrio spp

Genetic and Biochemical Characterization of VMB‐1, a Novel Metallo‐β‐Lactamase Encoded by a Conjugative, Broad‐Host Range IncC Plasmid from Vibrio spp

Spread of OXA-48 and NDM-1-Producing Klebsiella pneumoniae ST48 and ST101 in Chicken Meat in Western Algeria

Complex Class 1 Integron in a Clinical Escherichia coli Strain From Vietnam Carrying Both mcr-1 and blaNDM–1

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Identification and Characterization of IncA/C Conjugative, bla NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin

Cited by 19 publications

References 10 publications

Genetic and Biochemical Characterization of VMB‐1, a Novel Metallo‐β‐Lactamase Encoded by a Conjugative, Broad‐Host Range IncC Plasmid from Vibrio spp

Genetic and Biochemical Characterization of VMB‐1, a Novel Metallo‐β‐Lactamase Encoded by a Conjugative, Broad‐Host Range IncC Plasmid from Vibrio spp

Spread of OXA-48 and NDM-1-Producing Klebsiella pneumoniae ST48 and ST101 in Chicken Meat in Western Algeria

Complex Class 1 Integron in a Clinical Escherichia coli Strain From Vietnam Carrying Both mcr-1 and blaNDM–1

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Identification and Characterization of IncA/C Conjugative, bla _NDM-1 -Bearing Plasmid in Vibrio alginolyticus of Food Origin