Identification and characterization of intestine microRNAs and targets in red swamp crayfish, Procambarus clarkii infected with white spot syndrome virus

Abstract

MicroRNAs (miRNAs) are small non-coding endogenous RNA molecules that play important roles in the innate immunity system of invertebrates, especially in the aspect of antivirus. In the present study, high-throughput small RNA Illumina sequencing systems were used to identify differentially expressed miRNAs (DEMs) from the intestines of Procambarus clarkii that were infected with white spot syndrome virus (WSSV). As a result, 39 known and 12 novel miRNAs were identified in both NG and WG small RNA libraries. Seven DEMs were determined to be involved in the antiviral innate immunity in the intestines of P. clarkii. The results of the target gene predictions of the DEMs showed that the putative target genes of these 7 DEMs are related to tight junctions, vascular smooth muscle contraction regulation of the actin cytoskeleton, focal adhesion, RNA transport, mRNA surveillance, viral carcinogenesis, and Salmonella infection. These results provide theoretical insights for future studies on the antiviral immunity of crustaceans.

Vasodilator-stimulated phosphoprotein (VASP), a novel target of miR-4455, promotes gastric cancer cell proliferation, migration, and invasion, through activating the PI3K/AKT signaling pathway

“…Recently, miRNAs have emerged as novel regulatory of the actin cytoskeleton, via the regulation of cell growth and motility [ 42 , 43 ]. For example, miR-146a targets ROCK1 which affects cell movement in androgen-independent prostate cancer [ 44 ]; miR-155 is associated with TGF-induced RhoA suppression, leading to the dissolution of tight junctions between cells [ 45 ].…”

Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing

“…Expression profile of miRNAs changed a lot in pathological state or environmental factor stimulation. Previous studies have reported the miRNA profiles in P. clarkia, for example, Wang et al and Du et al identified the miRNAs in gills, intestines and lymph organs of P. clarkia infected with white spot syndrome virus [13][14][15]. Ou et al screened the miRNAs potentially related to immunity against Spiroplasma eriocheiris infection in P. clarkia [16].…”