Identification and Characterization of Jasmonic Acid Biosynthetic Genes in Salvia miltiorrhiza Bunge

Xue, Xiaoshan; Li, Runqing; Zhang, Caijuan; Li, Wenna; Li, Lin; Hu, Suying; Niu, Junfeng; Cao, Xiaoyan; Wang, Dong-Hao; Wang, Zhezhi

doi:10.3390/ijms23169384

Cited by 7 publications

(3 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AOS and AOC then modify it to produce 12-oxo-phytodienoic acid, a direct precursor of JA. JA is synthesized in peroxisomes through OPR3 [ 63 ]. This reduction step is critical for the subsequent steps in JA biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Endogenous Hormone Levels and Transcriptomic Analysis Reveal the Mechanisms of Bulbil Initiation in Pinellia ternata

Mou,

Zhang,

Qiu

et al. 2024

IJMS

View full text Add to dashboard Cite

Pinellia ternata is a medicinal plant that has important pharmacological value, and the bulbils serve as the primary reproductive organ; however, the mechanisms underlying bulbil initiation remain unclear. Here, we characterized bulbil development via histological, transcriptomic, and targeted metabolomic analyses to unearth the intricate relationship between hormones, genes, and bulbil development. The results show that the bulbils initiate growth from the leaf axillary meristem (AM). In this stage, jasmonic acid (JA), abscisic acid (ABA), isopentenyl adenosine (IPA), and salicylic acid (SA) were highly enriched, while indole-3-acetic acid (IAA), zeatin, methyl jasmonate (MeJA), and 5-dexoxystrigol (5-DS) were notably decreased. Through OPLS-DA analysis, SA has emerged as the most crucial factor in initiating and positively regulating bulbil formation. Furthermore, a strong association between IPA and SA was observed during bulbil initiation. The transcriptional changes in IPT (Isopentenyltransferase), CRE1 (Cytokinin Response 1), A-ARR (Type-A Arabidopsis Response Regulator), B-ARR (Type-B Arabidopsis Response Regulator), AUX1 (Auxin Resistant 1), ARF (Auxin Response Factor), AUX/IAA (Auxin/Indole-3-acetic acid), GH3 (Gretchen Hagen 3), SAUR (Small Auxin Up RNA), GA2ox (Gibberellin 2-oxidase), GA20ox (Gibberellin 20-oxidase), AOS (Allene oxide synthase), AOC (Allene oxide cyclase), OPR (Oxophytodienoate Reductase), JMT (JA carboxy l Methyltransferase), COI1 (Coronatine Insensitive 1), JAZ (Jasmonate ZIM-domain), MYC2 (Myelocytomatosis 2), D27 (DWARF27), SMAX (Suppressor of MAX2), PAL (Phenylalanine Ammonia-Lyase), ICS (Isochorismate Synthase), NPR1 (Non-expressor of Pathogenesis-related Genes1), TGA (TGACG Sequence-specific Binding), PR-1 (Pathogenesis-related), MCSU (Molybdenium Cofactor Sulfurase), PP2C (Protein Phosphatase 2C), and SnRK (Sucrose Non-fermenting-related Protein Kinase 2) were highly correlated with hormone concentrations, indicating that bulbil initiation is coordinately controlled by multiple phytohormones. Notably, eight TFs (transcription factors) that regulate AM initiation have been identified as pivotal regulators of bulbil formation. Among these, WUS (WUSCHEL), CLV (CLAVATA), ATH1 (Arabidopsis Thaliana Homeobox Gene 1), and RAX (Regulator of Axillary meristems) have been observed to exhibit elevated expression levels. Conversely, LEAFY demonstrated contrasting expression patterns. The intricate expression profiles of these TFs are closely associated with the upregulated expression of KNOX(KNOTTED-like homeobox), suggesting a intricate regulatory network underlying the complex process of bulbil initiation. This study offers a profound understanding of the bulbil initiation process and could potentially aid in refining molecular breeding techniques specific to P. ternata.

show abstract

Section: Discussionmentioning

confidence: 99%

Endogenous Hormone Levels and Transcriptomic Analysis Reveal the Mechanisms of Bulbil Initiation in Pinellia ternata

Mou,

Zhang,

Qiu

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…Lipoxygenases have been the subject of intensive study in various organisms, playing a critical role in growth and development, signaling molecule generation, stress response, and plant defense [3,17,38,39]. Through the utilization of HMM and BlastP combining the annotation data from yellow horn, fifteen potential XsLOX genes have been uncovered, surpassing those found in Arabidopsis thaliana [40] and tea plant [41], foxtail millet [16], tartary buck wheat [42], Passiflora Edulis [27], tomato [12], maize [43], but still be less than those discovered in Salvia miltiorrhiza bung [44], banana [45], apple [9], cucumber [39], cotton [15] and Populus [46]. An evolutionary analysis revealed that the four XsLOX proteins comprising XsLOX2/3/4/ 13/14 assemble into the 9-LOX subfamily; XsLOX1 falls under the 13-LOX I subfamily, with XsLOX5/6/7/8/9/10/11/12/15 conforming to the 13-LOX II subfamily [5,9].…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of lipoxygenase (LOX) genes involved in abiotic stresses in yellow horn

Hu,

Zhang,

Guo

2023

PLoS ONE

View full text Add to dashboard Cite

Lipoxygenase (LOX) gene plays an essential role in plant growth, development, and stress response. 15 LOX genes were identified, which were unevenly distributed on chromosomes and divided into three subclasses in this study. In promoter region analysis, many cis-elements were identified in growth and development, abiotic stress response, hormonal response, and light response. qRT-PCR showed that the LOX gene showed tissue specificity in seven tissues, especially XsLOX1, 3, and 7 were relatively highly expressed in roots, stems, and axillary buds. The different expression patterns of LOX genes in response to abiotic stress and hormone treatment indicate that different XsLOX genes have different reactions to these stresses and play diversified roles. This study improves our understanding of the mechanism of LOX regulation in plant growth, development, and stress and lays a foundation for further analysis of biological functions.

show abstract

“…Lipoxygenases (LOXs; EC 1.13.11.12) involve a group of non-heme iron enzymes that catalyze the deoxygenation of polyunsaturated fatty acids (PUFAs) into the corresponding lipid hydroperoxides (LOOHs). These products have numerous roles as growth regulators, antimicrobials and antifungals (aldehydes or divinyl ethers), flavors, and as signal molecules such as jasmonates [1][2][3]. Thus, LOXs are involved in diverse physiological plant functions including seed germination, plant growth and development, and fruit ripening and senescence [1,[4][5][6], but they are also involved in the mechanism of response to diverse environmental stresses [7,8].…”

Section: Introductionmentioning

confidence: 99%

Soybean (Glycine max L.) Lipoxygenase 1 (LOX 1) Is Modulated by Nitric Oxide and Hydrogen Sulfide: An In Vitro Approach

González‐Gordo

Lopéz-Jaramillo

Palma

et al. 2023

IJMS

View full text Add to dashboard Cite

Hydrogen sulfide (H2S) and nitric oxide (NO) are two relevant signal molecules that can affect protein function throughout post-translational modifications (PTMs) such as persulfidation, S-nitrosation, metal-nitrosylation, and nitration. Lipoxygenases (LOXs) are a group of non-heme iron enzymes involved in a wide range of plant physiological functions including seed germination, plant growth and development, and fruit ripening and senescence. Likewise, LOXs are also involved in the mechanisms of response to diverse environmental stresses. Using purified soybean (Glycine max L.) lipoxygenase type 1 (LOX 1) and nitrosocysteine (CysNO) and sodium hydrosulfide (NaHS) as NO and H2S donors, respectively, the present study reveals that both compounds negatively affect LOX activity, suggesting that S-nitrosation and persulfidation are involved. Mass spectrometric analysis of nitrated soybean LOX 1 using a peroxynitrite (ONOO−) donor enabled us to identify that, among the thirty-five tyrosine residues present in this enzyme, only Y214 was exclusively nitrated by ONOO−. The nitration of Y214 seems to affect its interaction with W500, a residue involved in the substrate binding site. The analysis of the structure 3PZW demonstrates the existence of several tunnels that directly communicate the surface of the protein with different internal cysteines, thus making feasible their potential persulfidation, especially C429 and C127. On the other hand, the CysNO molecule, which is hydrophilic and bulkier than H2S, can somehow be accommodated throughout the tunnel until it reaches C127, thus facilitating its nitrosation. Overall, a large number of potential persulfidation targets and the ease by which H2S can reach them through the diffuse tunneling network could be behind their efficient inhibition.

show abstract

Identification and Characterization of Jasmonic Acid Biosynthetic Genes in Salvia miltiorrhiza Bunge

Cited by 7 publications

References 89 publications

Endogenous Hormone Levels and Transcriptomic Analysis Reveal the Mechanisms of Bulbil Initiation in Pinellia ternata

Endogenous Hormone Levels and Transcriptomic Analysis Reveal the Mechanisms of Bulbil Initiation in Pinellia ternata

Identification and characterization of lipoxygenase (LOX) genes involved in abiotic stresses in yellow horn

Soybean (Glycine max L.) Lipoxygenase 1 (LOX 1) Is Modulated by Nitric Oxide and Hydrogen Sulfide: An In Vitro Approach

Contact Info

Product

Resources

About