Identification and characterization of Lactococcus starter strains in milk-based traditional fermented products in the region of Iran

Rahmati, Farzad

doi:10.3934/agrfood.2018.1.12

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…Biofilms exhibit significant resistance to antibiotics, host immune responses, desiccation, and UV light, enabling them to persist in challenging environments (34). The formation of biofilms and the development of antibiotic resistance create favorable conditions for A. baumannii to cause respiratory tract infections among hospitalized patients and military personnel (35).…”

Section: Discussionmentioning

confidence: 99%

Expression of Biofilm-Related Genes in Extensively Drug-Resistant Acinetobacter baumannii

Khosravy,

Hosseini,

Razavi

et al. 2023

Jundishapur J Microbiol

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Background: Acinetobacter baumannii is an important cause of nosocomial pneumonia in patients requiring long-term mechanical ventilation. Besides, extensively drug-resistant (XDR) strains cause infection in intensive care unit (ICU) patients. Chronic infections of A. baumannii and antimicrobial resistance are associated with biofilm formation. Several virulence genes, such as blaPER-1, pgaA, and bap, are involved in biofilm formation. Objectives: The current study examines the expression levels of biofilm formation-related genes in pneumonia patients. Methods: The sputum samples were collected from patients hospitalized in the ICU, and A. baumannii ATCC 19606, the reference strain, was isolated and cultured on blood agar, eosin methylene blue agar, and chocolate agar medium. The media were then incubated at 37°C for 18 - 24 hours. Next, Gram-Thirty XDR A. baumannii isolates were collected from the sputum samples of ICU patients at Besat Hospital in Tehran, Iran. Bacterial isolates were characterized for antibiotic resistance patterns and biofilm-forming ability. Subsequently, RNA was extracted from the biofilm-forming isolates. A real-time polymerase chain reaction (PCR) assay was performed to evaluate the expression levels of the blaPER-1, pgaA, and bap genes. Transcripts were normalized to 16S rRNA as an internal control, and gene expression fold changes were calculated. Statistical analysis was performed using an unpaired two-tailed t-test (P < 0.05) with SPSS (V. 16). Results: The disk diffusion susceptibility test revealed that all 30 (100%) isolates were resistant to piperacillin-tazobactam, cefepime, ceftriaxone, ceftazidime, gentamicin, imipenem, meropenem, levofloxacin, and ciprofloxacin. All 30 isolates from ICU-admitted patients (100%) were classified as XDR, and 27 (90%) isolates demonstrated the ability to form biofilms. The obtained results indicated a significant difference in gene expression levels. The fold change in expression for blaPER-1, bap, and pgaA was 7.473, 11.964, and 5.277, respectively. Conclusions: In our study, XDR A. baumannii primarily caused ventilator-associated pneumonia, and an observed increase in the expression of biofilm-related genes was noted in these strains. Healthcare centers should implement appropriate infection control programs to manage nosocomial infections, particularly in the ICU.

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Section: Discussionmentioning

confidence: 99%

Expression of Biofilm-Related Genes in Extensively Drug-Resistant Acinetobacter baumannii

Khosravy,

Hosseini,

Razavi

et al. 2023

Jundishapur J Microbiol

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“…Probiotics must survive sufficient against extreme conditions in the body and they can be easily replicated in the gut and reveal their health benefits to the host such as intestinal system maintenance, immune system stimulation, cholesterol, blood pressure regulation, and anticancer effects. The health features of the probiotic would be revealed when they are consumed alive and sufficiently 10 6 -10 7 CFU/g to reach the colon (Rahmati 2018a). Thus, the stability of probiotics strains against the environmental circumstances of the product and the digestive system is particularly significant.…”

Section: Different Coating Methods Used To Encapsulate Probioticmentioning

confidence: 99%

New insights into the role of nanotechnology in microbial food safety

et al. 2020

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Today, the role of nanotechnology in human life is undeniable as a broad range of industries, particularly food and medicine sectors, have been dramatically influenced. Nanomaterials can contribute to food safety by forming new nano-sized ingredients with modified physicochemical characteristics. Nanotechnologies can inhibit the growth of food spoilage microorganisms by recruiting novel and unique agents that are involved in removal of microbes from foods or prevent adhesion of microbial cells to food surfaces. Hence, nanotechnology could be considered as a high-potential tool in food packaging, safety, and preservation. Moreover, the prevention of biofilm formation by disturbing the attachment of bacteria to the food surface is another useful nanotechnological approach. Recently, nanoparticle-based biosensors have been designed and developed to detect the food-borne pathogens and hazardous substances through complicated mechanisms. During the past half-century, many methods such as freeze-drying and spray drying have been employed for increasing the viability in food industries; however, the other novel approaches such as encapsulation methods have also been developed. Admittedly, some beneficial bacteria such as probiotics bring diverse benefits for human health if only they are in a sufficient number and viability in the food products and gastrointestinal tract (GI). Encapsulation of these valuable microbial strains by nanoparticles improves the survival of probiotics under harsh conditions such as extreme levels of temperature, pH, and salinity during the processing of food products and within the GIT tract. The survival and effectiveness of encapsulated microorganisms depends on different factors including function of cell wall components in bacteria and type of coating materials. This review aims to broadly explore the potential of different aspects of nanotechnology in food industry, especially for packaging, preservation, safety, and viability.

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“…These strains are considered as the beneficial live microbial supplements in food products which incredibly improve the function of the intestine in human through cooperating with prebiotics. These valuable strains have a potential to be inserted in various sorts of food, drugs and nutritional supplements (Rahmati 2018a). The mechanism of action of probiotics is probably due to the competitive elimination of pathogens that inhibit the formation of pathogens in the host gastrointestinal tract by competing for nutrients or binding sites, altering the metabolism of bacteria, or by stimulating the immune system (Huang et al 2016;Rijkers et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Microencapsulation of Lactobacillus acidophilus and Lactobacillus plantarum in Eudragit S100 and alginate chitosan under gastrointestinal and normal conditions

Rahmati

2019

Appl Nanosci

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The main aim of the present study was the investigation of the viability of two probiotics bacteria of Lactobacillus acidophilus and Lactobacillus plantarum in different environments including simulated gastrointestinal and circumstances namely yogurt. For coating of strains, the double-coating technique was used as the first coating was alginate chitosan and second was Eudragit S100 and the results compared with free-coating cells. the number of free L. acidophilus and L. plantarum were 6.4 × 10 7 ± 3.3 × 10 4 and 7.5 × 10 7 ± 3.5 × 10, respectively, and after 32 days in the last measurement, a sharp decrease in the numbers was observed as they reached to 4.6 × 10 4 ± 0.9 × 10 2 and 1.5 × 10 2 ± 7.9 × 10 1 , respectively. In double-coated condition more number of strains survived as L.

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Identification and characterization of Lactococcus starter strains in milk-based traditional fermented products in the region of Iran

Cited by 6 publications

References 7 publications

Expression of Biofilm-Related Genes in Extensively Drug-Resistant Acinetobacter baumannii

Expression of Biofilm-Related Genes in Extensively Drug-Resistant Acinetobacter baumannii

New insights into the role of nanotechnology in microbial food safety

Microencapsulation of Lactobacillus acidophilus and Lactobacillus plantarum in Eudragit S100 and alginate chitosan under gastrointestinal and normal conditions

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