Identification and characterization of lipopolysaccharide-induced TNF-alpha factor gene from Japanese flounder Paralichthys olivaceus

Li, Shuo; Li, Xuejing; Gen, Xuyun; Chen, Yue; Jun-li, Wei; Sun, Jinsheng

doi:10.1016/j.vetimm.2013.11.006

Cited by 33 publications

(12 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Characterization and expression of TNFα have been investigated in various fish species such as bluefin tuna Thunnus orientalis [53] and grass carp Ctenopharyngodon idella [54]. In Japanese flounder, injection of head kidney primary cells with LPS and poly(I:C) up-regulated TNFα at 2-24 hpi [55]. Similarly, in the present study, TNFα was significantly up-regulated by FKC of V. anguillarum at 3 hpi in the three species.…”

Section: Discussionsupporting

confidence: 68%

Development of consensus qPCR primers to detect cytokine genes in three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili

Jirapongpairoj

Kobayashi

Fukuda³

et al. 2015

Fish Sci

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 68%

Development of consensus qPCR primers to detect cytokine genes in three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili

Jirapongpairoj

Kobayashi

Fukuda³

et al. 2015

Fish Sci

View full text Add to dashboard Cite

show abstract

“…Japanese flounder head kidney primary cells were prepared as described by Li et al [23]. Briefly, head kidneys were dissected and gently passed through a 40 µm sterile cell strainer (BD Biosciences) with a glass homogenizer and rinsed twice with DMEM-F12 medium (Invitrogen) containing 100 U/ml penicillin-streptomycin and 10 U/ml heparin (Sigma).…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization and Expression Analysis of ATP-Gated P2X7 Receptor Involved in Japanese Flounder (Paralichthys olivaceus) Innate Immune Response

Coddou

et al. 2014

PLoS ONE

Self Cite

View full text Add to dashboard Cite

ATP-gated P2X7 receptor (P2RX7) channel is a key component for purinergic signaling and plays important roles in the innate immune response in mammals. However, the expression, molecular properties and immune significances of P2RX7 in lower vertebrates are still very limited. Here we identified and characterized a novel bony fish P2RX7 homologue cDNA, termed poP2RX7, in Japanese flounder (Paralichthys olivaceus). PoP2RX7 protein shares about 60–88% sequence similarity and 45–78% sequence identity with known vertebrate P2RX7 proteins. Phylogenetic analysis placed poP2RX7 and other P2RX7 proteins within their own cluster apart from other P2RX members. While the functional poP2RX7 channel shares structural features in common with known P2RX7 homologs, electrophysiological studies revealed that BzATP, the more potent agonist for known mammalian and fish P2RX7s, shows similar potency to ATP in poP2RX7 activation. poP2RX7 mRNA constitutively expressed in all examined tissues from unstimulated healthy Japanese flounder with dominant expression in hepatopancreas and the lowest expression in head kidney, trunk kidney, spleen and gill. poP2RX7 mRNA expression, however, was significantly induced in Japanese flounder head kidney primary cells by Poly(I:C) and bacterial endotoxin LPS stimulations. In vivo experiments further revealed that poP2RX7 gene expression was substantially up-regulated by immune challenge with infectious bacteria Edwardsiella tarda and Vibrio anguillarum. Moreover, activation of poP2RX7 results in an increased gene expression of multifunctional cytokines IL-1β and IL-6 in the head kidney primary cells. Collectively, we identified and characterized a novel fish P2RX7 homolog which is engaged in Japanese flounder innate immune response probably through modulation of pro-inflammatory cytokines expression.

show abstract

“…One putative polyadenylation signal (AATAAA) was recognized at the nucleotide position 906, which was 21 nucleotides upstream of the poly (A) tail. Furthermore, there were two cytokine RNA instability motifs (ATTTA) at the 3′-UTR of Malitaf , which were also presented in the litaf gene of Paralichthys olivaceus [28]. This cDNA sequence has been deposited in the GenBank database under accession number KX421367.…”

Section: Resultsmentioning

confidence: 99%

“…Since then, a large amount of LITAF homologues have been obtained in several aquatic animals including mollusk [18,19,20,21,22,23], arthropod [24,25], sea cucumber ( Apostichopus japonicus ) [26], and amphioxus ( Branchiostoma belcheri ) [27], suggesting a conserved function in innate immunity. Although the litaf gene has been characterized in several fish species, the knowledge of the LITAF orthologs in most teleosts is still limited [28,29,30,31]. …”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Lipopolysaccharide Induced TNFα Factor from Blunt Snout Bream, Megalobrama amblycephala

Xiao

Jiang

et al. 2017

IJMS

View full text Add to dashboard Cite

Lipopolysaccharide induced TNFα factor (LITAF) is an important transcription factor responsible for regulation of tumor necrosis factor α. In this study, a novel litaf gene (designated as Malitaf) was identified and characterized from blunt snout bream, Megalobrama amblycephala. The full-length cDNA of Malitaf was of 956 bp, encoding a polypeptide of 161 amino acids with high similarity to other known LITAFs. A phylogenetic tree also showed that Malitaf significantly clustered with those of other teleost, indicating that Malitaf was a new member of fish LITAF family. The putative maLITAF protein possessed a highly conserved LITAF domain with two CXXC motifs. The mRNA transcripts of Malitaf were detected in all examined tissues of healthy M. amblycephala, including kidney, head kidney, muscle, liver, spleen, gill, and heart, and with the highest expression in immune organs: spleen and head kidney. The expression level of Malitaf in spleen was rapidly up-regulated and peaked (1.29-fold, p < 0.05) at 2 h after lipopolysaccharide (LPS) stimulation. Followed the stimulation of Malitaf, Matnfα transcriptional level was also transiently induced to a high level (51.74-fold, p < 0.001) at 4 h after LPS stimulation. Taken together, we have identified a putative fish LITAF ortholog, which was a constitutive and inducible immune response gene involved in M. amblycephala innate immunity during the course of a pathogenic infection.

show abstract

Identification and characterization of lipopolysaccharide-induced TNF-alpha factor gene from Japanese flounder Paralichthys olivaceus

Cited by 33 publications

References 16 publications

Development of consensus qPCR primers to detect cytokine genes in three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili

Development of consensus qPCR primers to detect cytokine genes in three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili

Molecular Characterization and Expression Analysis of ATP-Gated P2X7 Receptor Involved in Japanese Flounder (Paralichthys olivaceus) Innate Immune Response

Identification and Characterization of Lipopolysaccharide Induced TNFα Factor from Blunt Snout Bream, Megalobrama amblycephala

Contact Info

Product

Resources

About