Identification and Characterization of Megalocytivirus Type 3 Infection with Low Mortality in Starry Flounder, <i>Platichthys stellatus</i>, in Korea

Jin, Ji Woong; Kim, Yi Kyung; Kim, Young Chul; Kwon, Woo Ju; Jeong, Hyun Do

doi:10.1111/jwas.12439

Cited by 13 publications

(6 citation statements)

References 31 publications

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“…Moreover, it is known to show pathogenicity regardless of the environment and species when it infects a susceptible host (Lee et al, 2020). Our result demonstrated a different Megalocytivirusinfection in starry flounder compared to that in previous reports (Park et al, 2012;Won et al, 2013;Jung et al, 2016;Jin et al, 2018). Thus, starry flounder can be considered to show susceptibility to the RSIV and TRBIV genotypes.…”

Section: Evaluation Of Newly Designed Multiplex Pcr Using Field Samplescontrasting

confidence: 46%

“…RSIV infection is a viral disease that causes massive mortality in rock bream mariculture. Recently, studies have reported the RSIV infection of turbot, olive flounder, and starry flounder (Takano et al, 2001;Shi et al, 2004;Jeong et al, 2005;Won et al, 2013;Jin et al, 2018).…”

Section: Evaluation Of Newly Designed Multiplex Pcr Using Field Samplesmentioning

confidence: 99%

“…However, the current primer set of the OIE has not confirmed the specific detection of the TRBIV genotype. TRBIV infection has increased in recent decades in South Korea (Park et al, 2012;Won et al, 2013;Jung et al, 2016;Jin et al, 2018). Therefore, a suitable PCR-based detection method is essential for TRBIV detection.…”

Section: Introductionmentioning

confidence: 99%

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Improved multiplex PCR method for the detection of diverse Megalocytivirus in the Korea

Sohn

Lee

Kwon

et al. 2021

Preprint

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Megalocytivirus is a genus of piscine viruses that belongs to the Iridoviridae family, and this family comprises red seabream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), and infectious spleen and kidney necrosis virus. RSIV causes high mortality and economic losses in the rock bream (Oplegnathus fasciatus) Korean aquaculture industry. The World Organization for Animal Health’s Office International des Epizooties has provided a manual for RSIV detection. However, detection against TRBIV has not been confirmed. In this study, a multiplex PCR method was established to detect two genotypes of Megalocytivirus, RSIV and TRBIV. New primer pairs were optimized for the PCR reaction. A mixture of one universal and two specific primer pairs could amplify three distinct products targeting three different genes. The sensitivity of the primer pairs was evaluated and results showed a detection limit of 2.0  105 copies for each target gene. Moreover, the primer pairs did not amplify any other viruses. The evaluation of multiplex PCR using 21 RSIV Korean isolates has shown that it can distinguish two genotypes of Megalocytivirus from 21 RSIV Korean isolates. Finally, we describe a new multiplex PCR method to detect different genotypes of Megalocytivirus simultaneously, which makes the diagnosis of viral diseases occurring in the Korean aquaculture industry more convenient.

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Section: Evaluation Of Newly Designed Multiplex Pcr Using Field Samplescontrasting

confidence: 46%

Section: Evaluation Of Newly Designed Multiplex Pcr Using Field Samplesmentioning

confidence: 99%

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Improved multiplex PCR method for the detection of diverse Megalocytivirus in the Korea

Sohn

Lee

Kwon

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…For the analysis of viral infection level, each individual sample ( n = 20) was quantified with specific primer sets as published by Jin et al. (), targeting a fragment of the MCP region. This was carried out using the LightCycler 480II instrument (Roche, USA) according to the manufacturer's instructions.…”

Section: Pairwise Comparison Of Genetic Diversities Between Megalocytmentioning

confidence: 99%

A natural infection by the red sea bream iridovirus‐type Megalocytivirus in the golden mandarin fish Siniperca scherzeri

Kim

Hwang

Cho

et al. 2018

Journal of Fish Diseases

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An outbreak of a Megalocytivirus infection was found in the golden mandarin fish Siniperca scherzeri during September and October 2016, in Korea. Phylogeny and genetic diversity based on the major capsid protein (MCP) and adenosine triphosphatase (ATPase) genes showed a new strain. Designated as GMIV, this strain derived from the golden mandarin fish was suggested to belong to the red sea bream iridovirus (RSIV)-subgroup I. Additionally, this train clustered with the ehime-1 strain from red sea bream Pagrus major in Japan and was distinguished from circulating isolates (RSIV-type subgroup II and turbot reddish body iridovirus [TRBIV] type) in Korea. The infection level, evaluated by qPCR, ranged from 8.18 × 10 to 7.95 × 10 copies/mg of tissue individually, suggesting that the infected fish were in the disease-transmitting stage. The diseased fish showed degenerative changes associated with cytomegaly in the spleen as general sign of Megalocytivirus infection. The results confirm that the RSIV-type Megalocytivirus might have crossed the environmental and species barriers to cause widespread infection in freshwater fish.

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“…Supplementary Materials: Supplementary materials can be found at http://www.mdpi.com/1422-0067/21/5/1707/ s1. References [95][96][97][98][99][100]…”

mentioning

confidence: 99%

Co-Expression Network Analysis of Spleen Transcriptome in Rock Bream (Oplegnathus fasciatus) Naturally Infected with Rock Bream Iridovirus (RBIV)

Kim

Yoon

Lim

et al. 2020

IJMS

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Rock bream iridovirus (RBIV) is a notorious agent that causes high mortality in aquaculture of rock bream (Oplegnathus fasciatus). Despite severity of this virus, no transcriptomic studies on RBIV-infected rock bream that can provide fundamental information on protective mechanism against the virus have been reported so far. This study aimed to investigate physiological mechanisms between host and RBIV through transcriptomic changes in the spleen based on RNA-seq. Depending on infection intensity and sampling time point, fish were divided into five groups: uninfected healthy fish at week 0 as control (0C), heavy infected fish at week 0 (0H), heavy mixed RBIV and bacterial infected fish at week 0 (0MH), uninfected healthy fish at week 3 (3C), and light infected fish at week 3 (3L). We explored clusters from 35,861 genes with Fragments Per Kilo-base of exon per Million mapped fragments (FPKM) values of 0.01 or more through signed co-expression network analysis using WGCNA package. Nine of 22 modules were highly correlated with viral infection (|gene significance (GS) vs. module membership (MM) |> 0.5, p-value < 0.05). Expression patterns in selected modules were divided into two: heavy infected (0H and 0MH) and control and light-infected groups (0C, 3C, and 3L). In functional analysis, genes in two positive modules (5448 unigenes) were enriched in cell cycle, DNA replication, transcription, and translation, and increased glycolysis activity. Seven negative modules (3517 unigenes) built in this study showed significant decreases in the expression of genes in lymphocyte-mediated immune system, antigen presentation, and platelet activation, whereas there was significant increased expression of endogenous apoptosis-related genes. These changes lead to RBIV proliferation and failure of host defense, and suggests the importance of blood cells such as thrombocytes and B cells in rock bream in RBIV infection. Interestingly, a hub gene, pre-mRNA processing factor 19 (PRPF19) showing high connectivity (kME), and expression of this gene using qRT-PCR was increased in rock bream blood cells shortly after RBIV was added. It might be a potential biomarker for diagnosis and vaccine studies in rock bream against RBIV. This transcriptome approach and our findings provide new insight into the understanding of global rock bream-RBIV interactions including immune and pathogenesis mechanisms.

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Identification and Characterization of Megalocytivirus Type 3 Infection with Low Mortality in Starry Flounder, Platichthys stellatus, in Korea

Cited by 13 publications

References 31 publications

Improved multiplex PCR method for the detection of diverse Megalocytivirus in the Korea

Improved multiplex PCR method for the detection of diverse Megalocytivirus in the Korea

A natural infection by the red sea bream iridovirus‐type Megalocytivirus in the golden mandarin fish Siniperca scherzeri

Co-Expression Network Analysis of Spleen Transcriptome in Rock Bream (Oplegnathus fasciatus) Naturally Infected with Rock Bream Iridovirus (RBIV)

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