2002
DOI: 10.1021/tx010184a
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Identification and Characterization of Methylated and Ring-Fission Metabolites of Tea Catechins Formed in Humans, Mice, and Rats

Abstract: (-)-Epigallocatechin gallate (EGCG), the most abundant tea catechin, has been proposed to be beneficial to human health based on its strong antioxidative and other biological activities in vitro. Inadequate knowledge regarding the bioavailability and biotransformation of EGCG in humans, however, has limited our understanding of its possible beneficial health effects. In this study, 4',4' '-di-O-methyl-EGCG (4',4' '-DiMeEGCG) was detected in human plasma and urine by LC/MS/MS following green tea ingestion. Both… Show more

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Cited by 231 publications
(201 citation statements)
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“…Methylated catechins, most likely by COMT [213] also have been detected in the plasma [214]. Interestingly, as mentioned above, EGCG is a COMT inhibitor [144].…”
Section: Effects On Ttr Toxicity In Vivomentioning
confidence: 79%
“…Methylated catechins, most likely by COMT [213] also have been detected in the plasma [214]. Interestingly, as mentioned above, EGCG is a COMT inhibitor [144].…”
Section: Effects On Ttr Toxicity In Vivomentioning
confidence: 79%
“…Theasinensin A, 4"-methyl EGCG, and 4"-glucuronyl EGCG were synthesized previously in our laboratory [14][15][16]. Their structures were confirmed by 1 H, 13 C, and 2D NMR.…”
Section: Methodsmentioning
confidence: 99%
“…However, no EGCG autoxidation products were detected in the plasma samples from EGCG-treated mice. We have previously identified 4″-methyl EGCG as the major mono-methylated metabolite of EGCG in mice and 4″-glucuronyl EGCG as the major mono-glucuronide metabolite of EGCG in human, mouse, and rat microsomes [15,16]. In order to further confirm that 4″-glucuronyl EGCG was the major mono-glucuronide metabolite of EGCG in mouse plasma, we compared the LC/MS/MS spectrum of the standard 4″-glucuronyl EGCG with that of the corresponding peak in the plasma samples from EGCG-treated mice.…”
Section: Metabolite Profiles Of Egcg In the Plasma Samples From Egcg-mentioning
confidence: 99%
“…Previous in vivo studies suggest that the product profile of EGCG methylation is dose-dependent (Kida et al, 2000; Kohri et al, 2001;Meng et al, 2002). To further investigate this phenomena, concentration-dependent methylation of EGCG and 4Љ-MeEGCG by rat liver cytosol was studied using a 20-min incubation.…”
Section: Lc/ms/ms Analysis Of Methylated Catechinsmentioning
confidence: 98%
“…(Ϫ)-Epigallocatechin gallate (EGCG 1 ) and (Ϫ)-epigallocatechin (EGC) are considered to be the major effective constituents of green tea. The bioavailability and biotransformation of tea catechins are not well understood, even though several studies on this topic have been published (Chen et al, 1997;Yang et al, 1998;Li et al, 2001;Meng et al, 2001Meng et al, , 2002. After oral absorption, tea catechins undergo extensive methylation, glucuronidation, and sulfation Meng et al, 2001).…”
mentioning
confidence: 99%