2010
DOI: 10.1007/s11295-010-0319-5
|View full text |Cite
|
Sign up to set email alerts
|

Identification and characterization of microRNAs from citrus expressed sequence tags

Abstract: MicroRNAs (miRNAs) are a group of singlestranded noncoding RNAs with general size of 21-24 nucleotides, which negatively regulate gene expression posttranscriptionally by repressing gene translation or degrading targeted mRNAs. Despite the important functions of miRNAs in plants, little is known about miRNAs in citrus. Here we present a study of bioinformatics identification of microRNA precursors in citrus by comparing known plant miRNAs against 560,271 citrus ESTs. Seventy-eight ESTs from ten citrus species … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

1
7
0

Year Published

2010
2010
2019
2019

Publication Types

Select...
9

Relationship

1
8

Authors

Journals

citations
Cited by 17 publications
(8 citation statements)
references
References 72 publications
1
7
0
Order By: Relevance
“…Total RNA extraction and qRT-PCR was carried out as previously (Liu et al 2007;Wu et al 2010 Table 2). In order to determine qPCR efficiency of miRNAs and the reference gene actin, tenfold serial dilutions of cDNA template (0.01, 0.1, 1, 10, 100 ng/lL) was amplified.…”
Section: Qrt-pcr Of Mirnas and Target Genesmentioning
confidence: 99%
“…Total RNA extraction and qRT-PCR was carried out as previously (Liu et al 2007;Wu et al 2010 Table 2). In order to determine qPCR efficiency of miRNAs and the reference gene actin, tenfold serial dilutions of cDNA template (0.01, 0.1, 1, 10, 100 ng/lL) was amplified.…”
Section: Qrt-pcr Of Mirnas and Target Genesmentioning
confidence: 99%
“…The growing interest in the role of small RNAs has resulted in a large number of publications that describe the microRNA population of various crop plants, including citrus [18][19][20]. Yet, studies relating to the physiological roles Fig.…”
Section: Discussionmentioning
confidence: 99%
“…A wheat actin gene was selected to normalize the quantity of templates added in the PCR reactions for analyzing the expression of novel miRNAs using semi-quantitative RT-PCR [40]. Actin was also used as an endogenous control gene to normalize the expression of miRNAs in C. sinensis [41][42][43][44] and in tomato after inoculation with Phytophthora infestans [45]. Other stably expressed protein-coding genes, such as GAPDH or EF-1a, are also used as internal standards in miRNA quantitative studies [46,47].…”
Section: Introductionmentioning
confidence: 99%