Identification and characterization of miRNAs and target genes response to hypoosmotic adaption in the cobia,
            <i>Rachycentron canadum</i>

Huang, Baosong; Liu, Fubai; Cao, Danyu; Li, Gui-Ying; Lin, Kaiqi; Liu, Jinfeng; Su, Gang; Zhang, Jiandong; Wang, Bei; Huang, Jianbo; Pan, Chuanhao; Ma, Qian; Chen, Gang; Wang, Zhongliang

doi:10.1111/are.15177

Cited by 3 publications

(2 citation statements)

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“…Gonads are indispensable reproductive organs, and their development is commonly controlled by multiple sex-associated genes and pathways. With the rapid development of sequencing technology, RNA sequencing (RNA-Seq) of gonadal samples has been used to examine sex determination or differentiation in various fish species (Lin et al, 2017;Fu et al, 2020;Li et al, 2020;Chen et al, 2021;Lin et al, 2021;Gao et al, 2022). The transcriptomic information available in cobia is currently limited.…”

Section: Introductionmentioning

confidence: 99%

“…Piscicida ( Tran et al, 2018 ) and Streptococcus dysgalactiae ( Maekawa et al, 2019 ) are available. RNASeq has also been used to identify genes responsible for salinity regulation and adaptation in the gill of cobia ( Cao et al, 2020 ; Huang et al, 2021 ). In the present study, we screened differentially expressed genes (DEGs) between testes and ovaries in cobia via comparative gonad transcriptome analysis, and identified sex-associated key genes and pathways.…”

Section: Introductionmentioning

confidence: 99%

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Comparative gonad transcriptome analysis in cobia (Rachycentron canadum)

et al. 2023

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Background: Cobia (Rachycentron canadum) is a species of fish with high commercial potential particularly due to fast growth rates. The evidence of sexual size dimorphism favoring females indicate potential benefits in having a monosex culture. However, the involvement of genetic factors responsible for sexual development and gonadal maintenance that produces phenotypic sex in cobia is largely unknown.Methods: In the present study, we performed transcriptome sequencing of cobia to identify sex-biased significantly differentially expressed genes (DEGs) in testes and ovaries. The reliability of the gonad transcriptome data was validated by qPCR analysis of eight selected significantly differential expressed sex-related candidate genes.Results: This comparative gonad transcriptomic analysis revealed that 7,120 and 4,628 DEGs are up-regulated in testes or ovaries, respectively. Further functional annotation analyses identified 76 important candidate genes involved in sex determination cascades or sex differentiation, including 42 known testis-biased DEGs (dmrt1, amh and sox9 etc.), and 34 known ovary-biased DEGs (foxl2, sox3 and cyp19a etc.). Moreover, eleven significantly enriched pathways functionally related to sex determination and sex differentiation were identified, including Wnt signaling pathway, oocyte meiosis, the TGF-beta signaling pathway and MAPK signaling pathway.Conclusion: This work represents the first comparative gonad transcriptome study in cobia. The putative sex-associated DEGs and pathways provide an important molecular basis for further investigation of cobia’s sex determination, gonadal development as well as potential control breeding of monosex female populations for a possible aquaculture setting.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative gonad transcriptome analysis in cobia (Rachycentron canadum)

et al. 2023

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Identification of mapk genes, and their expression profiles in response to low salinity stress, in cobia (Rachycentron canadum)

Yang,

Ma,

Jin

et al. 2024

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Comprehensive Analysis of Whole-Transcriptome Profiles in Response to Acute Hypersaline Challenge in Chinese Razor Clam Sinonovacula constricta

Cao

Dong

Geng

et al. 2023

Biology

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The Chinese razor clam (Sinonovacula constricta) is an important for Chinese aquaculture marine bivalve that naturally occurs across intertidal and estuarine areas subjected to significant changes in salinity level. However, the information on the molecular mechanisms related to high salinity stress in the species remain limited. In this study, nine gill samples of S. constricta treated with 20, 30, and 40 ppt salinity for 24 h were used for whole-transcriptome RNA sequencing, and a regulatory network of competing endogenous RNAs (ceRNAs) was constructed to better understand the mechanisms responsible for adaptation of the species to high salinity. A total of 83,262 lncRNAs, 52,422 mRNAs, 2890 circRNAs, and 498 miRNAs were identified, and 4175 of them displayed differential expression pattern among the three groups examined. The KEGG analyses of differentially expressed RNAs evidenced that amino acid synthesis and membrane transport were the dominant factors involved in the adaptation of the Chinese razor clam to acute salinity increase, while lipid metabolism and signaling played only a supporting role. In addition, lncRNA/circRNA-miRNA-mRNA regulatory networks (ceRNA network) showed clearly regulatory relationships among different RNAs. Moreover, the expression of four candidate genes, including tyrosine aminotransferase (TAT), hyaluronidase 4 (HYAL4), cysteine sulfinic acid decarboxylase (CSAD), and ∆1-pyrroline-5-carboxylate synthase (P5CS) at different challenge time were detected by qRT-PCR. The expression trend of TAT and HYAL4 was consistent with that of the ceRNA network, supporting the reliability of established network. The expression of TAT, CSAD, and P5CS were upregulated in response to increased salinity. This might be associated with increased amino acid synthesis rate, which seems to play an essential role in adaptation of the species to high salinity stress. In contrast, the expression level of HYAL4 gene decreased in response to elevated salinity level, which is associated with reduction Hyaluronan hydrolysis to help maintain water in the cell. Our findings provide a very rich reference for understanding the important role of ncRNAs in the salinity adaptation of shellfish. Moreover, the acquired information may be useful for optimization of the artificial breeding of the Chinese razor clam under aquaculture conditions.

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Identification and characterization of miRNAs and target genes response to hypoosmotic adaption in the cobia, Rachycentron canadum

Cited by 3 publications

References 57 publications

Comparative gonad transcriptome analysis in cobia (Rachycentron canadum)

Comparative gonad transcriptome analysis in cobia (Rachycentron canadum)

Identification of mapk genes, and their expression profiles in response to low salinity stress, in cobia (Rachycentron canadum)

Comprehensive Analysis of Whole-Transcriptome Profiles in Response to Acute Hypersaline Challenge in Chinese Razor Clam Sinonovacula constricta

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