Identification and Characterization of Nine Novel Human Small GTPases Showing Variable Expressions in Liver Cancer Tissues

He, Hua; Dai, Fengwei; Liu, Yu; She, Xingyu; Zhao, Yong; Jiang, Jianmin; Chen, Xiaosong; Zhao, Shouyuan

doi:10.3727/000000002783992406

Cited by 123 publications

(102 citation statements)

References 26 publications

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“…Rab24 (part of the Ras superfamily) is involved in vesicle transport, and we found that it was elevated in dysplastic A/J ACF. The potential significance of this observation with respect to colorectal cancer is based in part on earlier reports demonstrating high levels of this protein in hepatocellular carcinomas, prompting speculation that Rab24 may represent a proto-oncogene (42). Protease inhibitors have been associated with attenuation of tumor progression and metastasis.…”

Section: Discussionmentioning

confidence: 99%

Genetic signatures of High- and Low-Risk Aberrant Crypt Foci in a Mouse Model of Sporadic Colon Cancer

et al. 2004

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To determine whether cancer risk is related to histopathological features of preneoplastic aberrant crypt foci (ACF), gene expression analysis was performed on ACF from two mouse strains with differing tumor sensitivity to the colonotropic carcinogen, azoxymethane. ACF from sensitive A/J mice were considered at high risk, whereas ACF from resistant AKR/J mice were considered at low risk for tumorigenesis. A/J and AKR/J mice received weekly injections of azoxymethane (10 mg/kg body weight), and frozen colon sections were prepared 6 weeks later. Immunohistochemistry was performed using biomarkers associated with colon cancer, including adenomatous polyposis coli, ␤-catenin, p53, c-myc, cyclin D1, and proliferating cell nuclear antigen. Hyperplastic ACF, dysplastic ACF, microadenomas, adjacent normal-appearing epithelium, and vehicle-treated colons were laser captured, and RNA was linearly amplified (LCM-LA) and subjected to cDNA microarray-based expression analysis. Patterns of gene expression were identified using adaptive centroid algorithm. ACF from low-and high-risk colons were not discriminated by immunohistochemistry, with the exception of membrane staining of ␤-catenin. To develop genetic signatures that predict cancer risk, LCM-LA RNA from ACF was hybridized to cDNA arrays. Of 4896 interrogated genes, 220 clustered into six broad clusters. A total of 226 and 202 genes was consistently altered in lesions from A/J and AKR/J mice, respectively. Although many alterations were common to both strains, expression profiles stratified high-and low-risk lesions. These data demonstrate that ACF with distinct tumorigenic potential have distinguishing molecular features. In addition to providing insight into colon cancer promotion, our data identify potential biomarkers for determining colon cancer risk in humans.

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Section: Discussionmentioning

confidence: 99%

Genetic signatures of High- and Low-Risk Aberrant Crypt Foci in a Mouse Model of Sporadic Colon Cancer

et al. 2004

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“…NO-mediated Ras activation has been shown to mediate signal transduction processes in cells including Raf 180 and Erk activation 181,182 leading to many different effects depending on the specific cells 180,[182][183][184][185] . It appears that Ras mutations do not normally occur in human hepatocellular carcinoma development 186 but it is quite clear that the Ras pathway is activated, however as evidenced by significantly enhanced Raf overexpression and enhanced activation 187,188 . The Raf kinase is an important target for anticancer therapeutics 172 …”

Section: Ras Pathway In Cancer Developmentmentioning

confidence: 99%

Nitric Oxide and Cancer Development

et al. 2007

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Abstract:The role of nitric oxide (NO) in cancer development has become a very active research area. This review presents an overview of many studies that implicate an important role of NO in the development of cancer. These include results in experimental models as well as many observations made on NO and inducible nitric oxide synthase (iNOS) in human cancers. Our survey of the literature has allowed us to conclude that in general large levels of NO will kill cancer cells but paradoxically at intermediate to lower levels NO prevents cancer cell apoptosis and enhances tumor growth. This basic tenet has been demonstrated in many experimental models. The mechanistic basis of how intermediate levels of NO mediate tumor development is an active area of research and is the subject of this review. NO mediated S-nitrosylation of key enzymes and regulatory proteins plays a critical role. Key proteins S-nitrosylated which enhance tumor development include several caspases involved in apoptosis, PTEN the tumor suppressor protein, Bcl-2 the mitochondrial protein which protects from apoptosis, OGG1 the DNA repair protein and methionine adenosyl transferase the liver protein which synthesizes adenosylmethionine. The S-nitrosylation of these proteins enhances DNA mutations, prevents cancer cell apoptosis and enhances oncogenic cell growth. (J Toxicol Pathol 2007; 20: 77-92)

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“…However, the incidence of breast cancer may also be associated with other unknown genes, and studies have suggested that the tumorigenesis and metastasis of certain cancer types is strongly associated with the loss of Rab gene function (10)(11)(12). The Rab family is the largest subfamily of the small GTP-binding protein Ras superfamily, which plays a regulatory role as a GTP-dependent molecular switch at different stages of vesicular transport (13).…”

Section: Discussionmentioning

confidence: 99%

Expression of WTH3 in breast cancer tissue and the effects on the biological behavior of breast cancer cells

Gan

Zuo

Wang

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the expression of WTH3 in tumor and normal breast tissue. The mRNA and protein expression levels of WTH3 were detected using reverse transcription quantitative polymerase chain reaction and western blot analysis, respectively. In addition, matrix metalloproteinase (MMP)-2 protein expression was measured. The effect of WTH3 expression on the proliferation activity of breast cancer cells was detected using a Cell Counting Kit-8 assay. Furthermore, the effects of WTH3 on the invasion and migration ability of the breast cancer cells was investigated. The results revealed that WTH3 was able to significantly inhibit the proliferation of the MCF-7 and MDA-MB-231 breast cancer cell lines. In addition, the invasion and migration assay demonstrated that WTH3 was able to inhibit the invasion and migration of breast cancer cells. Western blot analysis revealed that increased expression of WTH3 resulted in decreased expression levels of MMP-2, which has an important function in the metastasis of cancer cells. In conclusion, WTH3 expression differed between the tumor and normal breast tissues. WTH3 was able to inhibit the proliferation of breast cancer cells and decrease their invasion ability. Thus, WTH3 may be a promising target for breast cancer therapy in the future.

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Identification and Characterization of Nine Novel Human Small GTPases Showing Variable Expressions in Liver Cancer Tissues

Cited by 123 publications

References 26 publications

Genetic signatures of High- and Low-Risk Aberrant Crypt Foci in a Mouse Model of Sporadic Colon Cancer

Genetic signatures of High- and Low-Risk Aberrant Crypt Foci in a Mouse Model of Sporadic Colon Cancer

Nitric Oxide and Cancer Development

Expression of WTH3 in breast cancer tissue and the effects on the biological behavior of breast cancer cells

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