Identification and characterization of Phoma tracheiphila mutants impaired in pathogenicity following Agrobacterium-mediated mutagenesis

Kroitor-Keren, Tammy; Liarzi, Orna; Gat, T.; Skovorodnikova, Julia; Belausov, Eduard; Ezra, David

doi:10.1007/s12600-013-0328-7

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…The fungus was found to be very resistant to Hyg and was inhibited by the antibiotic only at 250 µg/ml (other fungal growth is usually inhibited at 75–100 µg/ml). Spore transformation was conducted as described by Kroitor‐Keren et al (2013) with slight modification: the top agar used for transformant selection was amended with 500 µg/ml Hyg instead of 150 µg/ml and the colonies growing above the top agar where transferred to PDA amended with 250 µg/ml Hyg instead of 150 µg/ml. Colonies growing on the selective medium (regrown at least three times) were tested for GFP expression by examination of the growing mycelium under a Nikon SMZ25 stereomicroscope equipped with a YFP‐2427B filter set (excitation 484–514 nm/dichroic beam‐splitter single‐edge 520 nm/emission 525–557 nm), a Nikon DS‐Ri2 camera, and the NIS Elements program (Nikon).…”

Section: Methodsmentioning

confidence: 99%

Aspergillus niger, the causal agent of black mould disease in date fruits, infects and colonizes flowers and young fruitlets

et al. 2021

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Black mould, caused by Aspergillus niger, is the main fungal disease affecting date fruits. Symptoms develop inside the fruit, at the inner space between the pulp and the seed. This study focuses on the aetiology of black mould disease in Medjoul fruit. We followed symptom appearance naturally and after artificial A. niger inoculation at different development and maturity stages. Symptoms developed in only a short period during early fruit ripening. However, fruits were commonly colonized at earlier stages of development. Artificial inoculation of flowers and setting fruitlets increased the level of fruit colonization, while fungicide spraying at these stages decreased colonization. Several weeks following flower inoculation by A. niger, mycelium could be detected on degenerating stigmas and carpels as well as between the fruit and the calyx. Following inoculation with an A. niger strain expressing green fluorescent protein (A. nigerGFP), the pathogen was detected on stigmas of setting fruitlets but not within the stigmatic tissue or the transmitting tract of the carpel. The A. nigerGFP strain was detected during fruit development below the base of the large fruitlets and above the calyx surrounding the vascular bundle leading into the fruit. The results suggest that A. niger can infect and colonize flowers and setting fruitlets, grows on the degenerating carpels, and remains latent at a protected site at the base of the fruit until ripening. It then induces the typical black mould symptoms. The significance of these results for developing means to cope with the disease is discussed.

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Section: Methodsmentioning

confidence: 99%

Aspergillus niger, the causal agent of black mould disease in date fruits, infects and colonizes flowers and young fruitlets

et al. 2021

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“…tracheiphila is present in an asymptomatic and latent infection, an apparently healthy plant may suddenly develop the disease and collapse [53]. Consequently, diagnosis of infection based on typical phenotypic symptoms is not suitable to estimate disease development and should be supported by molecular approaches for an earlier and certain detection in still symptomless plants [54,55]. The progress of the disease is basipetally, indeed P. tracheiphila invades the xylem progressively starting from the site of infection, proceeding slowly downward from the infected young shoots to the branches and main limbs, seizing the whole stem massively before foliar symptoms appeared [53][54][55].…”

Section: Msd In Citrus Genusmentioning

confidence: 99%

“…When the pathogen infects trunk and roots the tree dies. This movement is supported by phialoconidia, which move in the xylem by utilizing the transpiration flow of plants [55]. Since extensive virulence variability in P. tracheiphila causing MSD on citrus was detected [29], the pathogen may kill a lemon plant within a few months or years, depending on type/pathogenicity of isolate and, also, the age of tree.…”

Section: Msd In Citrus Genusmentioning

confidence: 99%

An Overview on Citrus Mal Secco Disease: Approaches and Strategies to Select Tolerant Genotypes in C. limon

2019

Crop Breed Genet Genom.

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Citrus is an economically important fruit crop that is seriously afflicted by several biotic stress, caused by both viruses and fungi, thus, new sustainable strategies to manage these diseases are needed. Lemon (Citrus limon (L.) Burm. f) is a tree in the Rutaceae family and after orange and mandarin, is the third most important Citrus species. Lemon fruits contain important bio-compounds and have central role in prevention of diseases of a balanced diet. Among C. limon diseases, "mal secco" is a highly destructive tracheomycotic fungal infection spread over the coasts of the Mediterranean basin and the Black Sea areas. The causal agent is the mitosporic fungus Phoma tracheiphila (Petri) Kantschaveli & Gikachvili (syn. Deuterophoma tracheiphila Petri). The pressure of disease has led to a marked decline of the Mediterranean's lemon crop acreage in the last 30 years. The present review explores the state of art in the struggle against mal secco disease (MSD), summarizing main results obtained from both conventional and unconventional breeding approaches. In addition, the strategies used to study the mechanisms involved in P. tracheiphila/lemon interaction and the results obtained to isolate key genes activates in tolerant genotypes are also described in depth. The overview of emergent approaches and technologies is focused to highlight the progress obtained using advanced lemon-breeding methods and suggest guidelines for future research.

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Diseases of Etrog Citron and Other Citrus Trees

Bar‐Joseph

Ezra

Licciardello³

et al. 2023

The Citron Compendium

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Identification and characterization of Phoma tracheiphila mutants impaired in pathogenicity following Agrobacterium-mediated mutagenesis

Cited by 5 publications

References 19 publications

Aspergillus niger, the causal agent of black mould disease in date fruits, infects and colonizes flowers and young fruitlets

Aspergillus niger, the causal agent of black mould disease in date fruits, infects and colonizes flowers and young fruitlets

An Overview on Citrus Mal Secco Disease: Approaches and Strategies to Select Tolerant Genotypes in C. limon

Diseases of Etrog Citron and Other Citrus Trees

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