Identification and characterization of phytochemicals and constituents in Desmodium velutinum stem using high-performance liquid chromatography (HPLC)

Nkwocha, Chinelo Chinenye; Ogugofor, Martins; Chukwuma, Ifeoma Felicia; Njoku, O.U.

doi:10.1016/j.prmcm.2022.100090

Cited by 10 publications

(4 citation statements)

References 25 publications

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“…Notably, the high amounts of alkaloids in the extracts evoke much research interest due to their biological activity and therapeutic potential. Alkaloids have anticancer, antioxidant, antimicrobial, anti-inflammatory, and immunomodulatory properties [ 32 , 37 ]. Collectively, the presence of these phytochemicals with immune modulatory effects suggests that the extracts could have promising potential in treating immune-related diseases.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of epiphytic Loranthus micranthus leaf extracts collected from two host plants: Psidium guajava and Parkia biglobosa

Idoko,

Chukwuma,

Nworah

et al. 2024

BMC Complement Med Ther

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Background Immunological abnormalities are implicated in the pathogenesis of many chronic diseases. Due to the drug-related adverse effects of currently available orthodox immunomodulators, natural immunomodulators are being looked upon as potential agents to replace them in therapeutic regimens. This research aimed to investigate the immunomodulatory potential of L. micranthus extracts epiphytic on Psidium guajava (LMPGE) and Parkia biglobosa (LMPBE). Methods Phytochemical screening and acute toxicity testing were carried out to identify the phytoconstituents and safety profiles of the extracts. The extracts’ innate and adaptive immunomodulatory potentials were determined in experimental animals using in vivo leucocyte mobilization, delayed-type hypersensitivity (DTH) response, hemagglutination antibody titre, and cyclophosphamide-induced myelosuppression models. Levamisole was used as the standard drug throughout the study. Results Compared to LMPBE, LMPGE contained significantly (p < 0.05) more tannins, cyanogenic glycosides, saponins, reducing sugars, glycosides, flavonoids, and alkaloids. Furthermore, the groups treated with the extracts had a significant (p < 0.05) increase in the total number of leucocytes, neutrophils, basophils, and antibody titers relative to the untreated control. In the same way, the treatment raised TLC in cyclophosphamide-intoxicated rats, with 250 mg/kg b. w. of LMPGE and LMPBE recording 9712.50 ± 178.00 and 8000.00 ± 105.00 × 109 /L, respectively, compared to 3425.00 ± 2 5.00 × 109 /L in the untreated group. Overall, LMPGE was more effective. Conclusions The findings from this study suggest that L. micranthus epiphytic in Psidium guajava and Parkia biglobosa has possible immune stimulating potential.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of epiphytic Loranthus micranthus leaf extracts collected from two host plants: Psidium guajava and Parkia biglobosa

Idoko,

Chukwuma,

Nworah

et al. 2024

BMC Complement Med Ther

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“…42 The anti-DM effects of rutin could also be linked to its strong antioxidant and anti-inflammatory activities. 47 Almost similarly, catechin exerts anti-diabetic effects by boosting antioxidant status and could ameliorate diabetic autonomic nephropathy. 48 Reports have shown that kaempferol inhibits gluconeogenesis and enhances glucose metabolism.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Characterization, Functional Nutrition, and Anti-Diabetic Potentials of Leptadenia hastata (pers) Decne Leaves: In Silico and In Vitro Studies

Chukwuma

Nworah

Apeh³

et al. 2022

Bioinform Biol Insights

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The geometrical increase in diabetes mellitus (DM) and the undesirable side effects of synthetic drugs have intensified efforts to search for an effective and safe anti-diabetic therapy. This study aimed to identify the antioxidant and anti-diabetic agents in the ethanol extract of Leptadenia hastata (EELH). The phytochemicals, antioxidant vitamins, and minerals present in EELH were determined using standard procedures to achieve this aim. Gas chromatography coupled with mass spectroscopy and flame ionization detector (GC-MS/GC-FID) was employed to identify bioactive compounds. An e-pharmacophore model was generated from the extra precision, and energy-minimized docked position of standard inhibitor, acarbose onto human pancreatic amylase (HPA, PDB-6OCN). It was used to screen the GC-MS/GC-FID library of compounds. The top-scoring compounds were subjected to glide XP-docking and prime MM-GBSA calculation with the Schrodinger suite-v12.4. The Adsorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) prediction of the best-fit compounds was made using SwissADME and PROTOX-II webservers. Further validation of the docking results was performed with the in vitro analysis of the α-amylase and α-glucosidase inhibitory activities. EELH contains appreciable amounts of antioxidant and anti-diabetic phytoconstituents. The top-4 scoring compounds (rutin, epicatechin, kaempferol, and naringenin) from the EELH phytochemical library interacted with amino acid residues within and around the HPA active site. The ADMET prediction shows that epicatechin, kaempferol, and naringenin had favorable drug-likeness, pharmacokinetic properties, and a good safety profile. EELH demonstrated good inhibitory actions against α-amylase and α-glucosidase with 1C50 values of 14.14 and 4.22 µg/mL, respectively. Thus, L hastata phytoconstituents are promising novel candidates for developing an anti-diabetic drug.

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“…The use of plants as therapeutic agents started during human evolution due to the presence of phytochemicals that have significant biological and pharmaceutical actions [11][12][13]. This study recorded a high abundance of phenols, alkaloids, flavonoids, and reducing sugars with moderate amounts of tannins and terpenoids in CPEE.…”

Section: Quantitative Phytochemical Screening Of the Cpeementioning

confidence: 99%

“…(Table 1). These phytochemicals that were identified in the extract have proven to be effective as antioxidant, anti-inflammatory, immunomodulatory, neuroprotective, and anti-diabetic agents [13]. Hence, their presence in the extract suggests that it could have a plethora of activities against the aforementioned diseases.…”

Section: Quantitative Phytochemical Screening Of the Cpeementioning

confidence: 99%

Modulatory Action of Phenolic-Enriched Combretum paniculatum Vent Ethanolic Extract on Oxidoinflammatory Anomalies in Experimental Animals

Chukwuma

Nworah

Apeh³

2023

The 2nd International Electronic Conference on Biomedicines

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Identification and characterization of phytochemicals and constituents in Desmodium velutinum stem using high-performance liquid chromatography (HPLC)

Cited by 10 publications

References 25 publications

Immunomodulatory effects of epiphytic Loranthus micranthus leaf extracts collected from two host plants: Psidium guajava and Parkia biglobosa

Immunomodulatory effects of epiphytic Loranthus micranthus leaf extracts collected from two host plants: Psidium guajava and Parkia biglobosa

Phytochemical Characterization, Functional Nutrition, and Anti-Diabetic Potentials of Leptadenia hastata (pers) Decne Leaves: In Silico and In Vitro Studies

Modulatory Action of Phenolic-Enriched Combretum paniculatum Vent Ethanolic Extract on Oxidoinflammatory Anomalies in Experimental Animals

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