Identification and characterization of plant resistance genes (R genes) in sorghum and their involvement in plant defense against aphids

Zhang, Hengyou; Huang, Jian; Huang, Yinghua

doi:10.1007/s10725-022-00797-x

Cited by 13 publications

(9 citation statements)

References 54 publications

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“…Furthermore, R genes play an important role in resistance, so we further identified 303 resistance-related genes in S. pinnatisectum. Another set of 68 genes, whose the expression patterns range from low to high with time lapse in the 12 RNAseq (Figure 4), may be involved in resistance to late blight disease, as verified by the expression profiles of these genes in the transcriptome data (Van Der Biezen and Jones, 1998;Pandolfi et al, 2017;Bezerra-Neto et al, 2020;Zhang et al, 2022). The Venn diagram shows that some of the genes that enhance over time in Cluster 1 are correlated with functional annotations, with 37.3% demonstrating a connection to the R genes and Cluster 1.…”

Section: Discussionmentioning

confidence: 84%

“…Moreover, the plant cell wall and innate immunity of each cell also provide disease resistance against plant–pathogen interactions ( Van Der Biezen and Jones, 1998 ; Dangl and Jones, 2001 ; Ausebel, 2005 ; Chisholm et al, 2006 ). Resistance (R) genes constitute a superfamily and famous genes are used to study disease resistance in plants ( Bergelson et al, 2001 ; Pandolfi et al, 2017 ; Zhang et al, 2022 ) to recognize the pathogen-derived virulence factors. These R genes can directly or indirectly recognize pathogen-derived virulence factors, thereby activating a series of disease-resistance signaling pathways that ultimately lead to plant protection against diseases ( Staskawicz et al, 2001 ; Abramovitch and Martin, 2004 ; Zhou and Chai, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification analysis in wild-type Solanum pinnatisectum reveals some genes defending against Phytophthora infestans

Shen,

Lu,

Yang

et al. 2024

Front. Genet.

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Solanum pinnatisectum exhibits strong resistance to late blight caused by Phytophthora infestans but only an incomplete genome assembly based on short Illumina reads has been published. In this study, we generated the first chromosome-level draft genome for the wild-type potato species S. pinnatisectum in China using Oxford Nanopore technology sequencing and Hi-C technology. The high-quality assembled genome size is 664 Mb with a scaffold N50 value of 49.17 Mb, of which 65.87% was occupied by repetitive sequences, and predominant long terminal repeats (42.51% of the entire genome). The genome of S. pinnatisectum was predicted to contain 34,245 genes, of which 99.34% were functionally annotated. Moreover, 303 NBS-coding disease resistance (R) genes were predicted in the S. pinnatisectum genome to investigate the potential mechanisms of resistance to late blight disease. The high-quality chromosome-level reference genome of S. pinnatisectum is expected to provide potential valuable resources for intensively and effectively investigating molecular breeding and genetic research in the future.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Genome-wide identification analysis in wild-type Solanum pinnatisectum reveals some genes defending against Phytophthora infestans

Shen,

Lu,

Yang

et al. 2024

Front. Genet.

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“…The authors proposed four SSR markers that could be used as markers of indirect selection. Using RNA sequencing and quantitative PCR [31], it was shown that after infestation of the resistant line PI550607 (VIR-924 according to the VIR catalogue) and the susceptible line Tx7000 with the aphid biotype Gb-I, the level of expression (overexpression) of fifteen genes in the resistant line and six genes in the susceptible one. The authors suggested that the Sobic.010G130700 gene coding RLPs (receptor-like proteins) and Sobic.003G325100 gene coding NBS protein, localized on chromosomes 10 and 3, are the most significant in the interaction of plants with aphids.…”

Section: Discussionmentioning

confidence: 99%

Newly Developed Restorer Lines of Sorghum [Sorghum bicolor (L.) Moench] Resistant to Greenbug

Radchenko,

Anisimova,

Ryazanova

et al. 2024

Plants

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Eight lines of grain sorghum [Sorghum bicolor (L.) Moench], which can be used as a promising source material in heterotic hybrid breeding as pollen fertility restorers and donors of resistance to the greenbug (Schizaphis graminum Rondani), are characterized. The new restorer lines (R-lines) were developed by crossing the maternal sterile line Nizkorosloe 81s (CMS A1) with two lines selected from the grain sorghum collection accessions VIR-928 and VIR-929 as the paternal forms. The R-lines were genotyped using PCR markers, and also characterized by height, duration of the seedling–flowering period, and some of the technological properties of flour. With the use of microsatellite markers linked to the Rf genes and by hybridological analysis, it was shown that the new lines carry the dominant allele of the gene Rf2. The PCoA analysis demonstrated clear differences of each R-line from the parents. The genotypes of the new lines and their parental forms for the Rf2 locus were confirmed by applying three allele-specific codominant CAPS markers which detected SNPs in the candidate Rf2 gene. All new lines were highly fertile, as demonstrated by cytological analysis of acetocarmine-stained pollen preparations. A high resistance to the greenbug was demonstrated for each new R-line both in the laboratory and field conditions against a severe aphid infestation. Grain quality parameters such as protein content and dough rheological properties varied widely and were quite satisfactory in some R-lines. Characteristics common to all eight sorghum lines studied, such as the ability to restore pollen fertility in the F1 generation, good pollen quality, greenbug resistance, early ripening, spreading panicle, and low stature, allow us to recommend them for producing commercial F1 hybrids with satisfactory grain quality for both fodder and food purposes.

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“…WRKY transcription factor 86 ( SbWRKY86 ) ( Sobic.009G238200 ) has also been identified in sorghum as a candidate gene involved in providing defense against SCA [ 35 ]. Furthermore, R -genes (plant resistance genes) in sorghum, for example, receptor-like kinase (RLK) genes, NBS-LRR, and receptor-like protein (RLP) genes have altered expression levels in the resistant sorghum line upon aphid infestation that can be implied for providing resistance to greenbugs [ 36 ]. Genome-wide association mapping of sorghum has revealed defense related genes like LRR, flavonoid biosynthesis, 12-oxo-phytodienoic reductase, WRKY transcription factors, lipoxygenases, and Avr proteins can be potential sources for sorghum breeding for aphid resistance [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Temporal transcriptomic profiling elucidates sorghum defense mechanisms against sugarcane aphids

Puri,

Grover,

Pingault

et al. 2023

BMC Genomics

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Background The sugarcane aphid (SCA; Melanaphis sacchari) has emerged as a key pest on sorghum in the United States that feeds from the phloem tissue, drains nutrients, and inflicts physical damage to plants. Previously, it has been shown that SCA reproduction was low and high on sorghum SC265 and SC1345 plants, respectively, compared to RTx430, an elite sorghum male parental line (reference line). In this study, we focused on identifying the defense-related genes that confer resistance to SCA at early and late time points in sorghum plants with varied levels of SCA resistance. Results We used RNA-sequencing approach to identify the global transcriptomic responses to aphid infestation on RTx430, SC265, and SC1345 plants at early time points 6, 24, and 48 h post infestation (hpi) and after extended period of SCA feeding for 7 days. Aphid feeding on the SCA-resistant line upregulated the expression of 3827 and 2076 genes at early and late time points, respectively, which was relatively higher compared to RTx430 and SC1345 plants. Co-expression network analysis revealed that aphid infestation modulates sorghum defenses by regulating genes corresponding to phenylpropanoid metabolic pathways, secondary metabolic process, oxidoreductase activity, phytohormones, sugar metabolism and cell wall-related genes. There were 187 genes that were highly expressed during the early time of aphid infestation in the SCA-resistant line, including genes encoding leucine-rich repeat (LRR) proteins, ethylene response factors, cell wall-related, pathogenesis-related proteins, and disease resistance-responsive dirigent-like proteins. At 7 days post infestation (dpi), 173 genes had elevated expression levels in the SCA-resistant line and were involved in sucrose metabolism, callose formation, phospholipid metabolism, and proteinase inhibitors. Conclusions In summary, our results indicate that the SCA-resistant line is better adapted to activate early defense signaling mechanisms in response to SCA infestation because of the rapid activation of the defense mechanisms by regulating genes involved in monolignol biosynthesis pathway, oxidoreductase activity, biosynthesis of phytohormones, and cell wall composition. This study offers further insights to better understand sorghum defenses against aphid herbivory.

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Identification and characterization of plant resistance genes (R genes) in sorghum and their involvement in plant defense against aphids

Cited by 13 publications

References 54 publications

Genome-wide identification analysis in wild-type Solanum pinnatisectum reveals some genes defending against Phytophthora infestans

Genome-wide identification analysis in wild-type Solanum pinnatisectum reveals some genes defending against Phytophthora infestans

Newly Developed Restorer Lines of Sorghum [Sorghum bicolor (L.) Moench] Resistant to Greenbug

Temporal transcriptomic profiling elucidates sorghum defense mechanisms against sugarcane aphids

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