Identification and Characterization of Promoters Regulating tuf Expression in Chlamydia trachomatis Serovar F

Chlamydia trachomatis σ 28 recognizes the fliC promoter of Escherichia coli and responds to heat shock in chlamydiae

“…Plasmid pMT504 (gift of Dr Ming Tan, University of California, Irvine, CA, USA) , which contained a longer promoterless G-less cassette and a shorter G-less cassette under the control of the promoter of chlamydial rrn P1, was used for cloning the P1 and P3 promoters of tuf (encoding translation elongation factor EF-Tu) (Shen et al, 2000). The P1 tuf and P3 tuf PCR products were generated using primer pairs Ptufpri-F/P1tufpri-R and PtufpriF/P3tufpriR (Table 2), digested with EcoRI and EcoRV and inserted into EcoRI and EcoRV sites of pMT504.…”

“…Primer extension was carried out as described previously (Shen et al, 2000). Synthetic oligonucleotide primers were complemented to chlamydial rpsD, covering 38-58 bp downstream sequence of the GTG translation initiation codon (fliA-pe1, Table 2) and of the G-less region of pMT504 (Gless-pe1, Table 2) by PCR.…”

“…Infected cells were lysed before or after exposure to 42 6C (water bath) for 10 min. Primer extension analysis was performed as described previously (Shen et al, 2000) using primer fliA-pe1 (Table 2). DNA sequence ladders (GATC) of pXE2 ( (Fig.…”

Molecular Epidemiology of Chlamydia trachomatis Urogenital Infection

“…The fully sequence C. trachomatis genome consist of a chromosome of approximately 1.0 Mbp plus an extrachromosomal plasmid of approximately 7.5 kbp, with a total of approximately 900 likely protein-coding genes (Read et al, 2000;Carlson et al, 2005) The transcriptional profile of the C. trachomatis genome has been analysed by microarrays and RT-PCR (Douglas & Hatch, 2000;Shen et al, 2000). The microarrays and RT-PCR The eukaryotic cell becomes infected when an EB adheres to the cytoplasmic membrane.…”

Discovery and Validation of New Regulatory RNA Elements in Chlamydia trachomatis

“…In general, most published σ 66 promoter elements have acceptable variation from the consensus (Engel and Ganem, 1987;Birkelund et al, 1989;Hatch, 1995, 1996;Ochiai et al, 1999;Shen et al, 2000;Schaumburg and Tan, 2003;Grech et al, 2007). Therefore; except for CTIG360 and CTIG498, the predicted promoters are σ 66 promoters.…”