Identification and characterization of resistance to rhizomania in an ecotype of Beta vulgaris subsp. maritima

Geyl, L.; Heriz, M. Garcia; Panchev, Valentin; Hehn, Alain; Merdinoglu, Didier

doi:10.1111/j.1365-3059.1995.tb02741.x

Cited by 14 publications

(8 citation statements)

References 28 publications

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“…BNYVV resistance is known to be widespread in wild beet B. vulgaris subsp. maritima (Whitney, 1989;Geyl et al, 1995), from which the three differential lines MR0, MR1 and MR2 were selected by foliar rub-inoculation (Tamada, 2007). The infection phenotype in MR2 plants was similar to that in Rizor plants (Table 1, Fig.…”

Section: Discussionmentioning

confidence: 99%

Identification of amino acids of the beet necrotic yellow vein virus p25 protein required for induction of the resistance response in leaves of Beta vulgaris plants

Chiba

Miyanishi

Andika

et al. 2008

Journal of General Virology

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The RNA3-encoded p25 protein of beet necrotic yellow vein virus (BNYVV) is responsible for the production of rhizomania symptoms of sugar beet roots (Beta vulgaris subsp. vulgaris). Here, it was found that the presence of the p25 protein is also associated with the resistance response in rub-inoculated leaves of sugar beet and wild beet (Beta vulgaris subsp. maritima) plants. The resistance phenotype displayed a range of symptoms from no visible lesions to necrotic or greyish lesions at the inoculation site, and only very low levels of virus and viral RNA accumulated. The susceptible phenotype showed large, bright yellow lesions and developed high levels of virus accumulation. In roots after Polymyxa betae vector inoculation, however, no drastic differences in virus and viral RNA accumulation levels were found between plants with susceptible and resistant phenotypes, except at an early stage of infection. There was a genotype-specific interaction between BNYVV strains and two selected wild beet lines (MR1 and MR2) and sugar beet cultivars. Sequence analysis of natural BNYVV isolates and site-directed mutagenesis of the p25 protein revealed that 3 aa residues at positions 68, 70 and 179 are important in determining the resistance phenotype, and that host-genotype specificity is controlled by single amino acid changes at position 68. The mechanism of the occurrence of resistance-breaking BNYVV strains is discussed.

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Section: Discussionmentioning

confidence: 99%

Identification of amino acids of the beet necrotic yellow vein virus p25 protein required for induction of the resistance response in leaves of Beta vulgaris plants

Chiba

Miyanishi

Andika

et al. 2008

Journal of General Virology

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“…Such genetic diversity in BNYVV resistance of B. vulgaris subsp. maritima was also observed in other studies in which the resistance to BNYVV was estimated on the basis of virus levels in plant roots grown in infested soils (Geyl et al 1995;Whitney 1989). The levels of virus in the roots were lower in plants with the non-YS phenotype compared with the YS phenotype plants, although the resistance response in leaves after rub-inoculation was much stronger than that in roots after inoculation with virus-carrying P. betae (T. Tamada unpublished data).…”

Section: Most Of the Plants Inoculated With These Virus Isolates Diedmentioning

confidence: 64%

“…It is well known that BNYVV resistance genes are derived from the wild beet B. vulgaris subsp. maritima accessions (Biancardi et al 2002;de Biaggi 1987;Geyl et al 1995;Lewellen et al 1987;Whitney 1989).…”

Section: Beet Necrotic Yellow Vein Virus (Bnyvv Genus Benyvirus)mentioning

confidence: 96%

Susceptibility and resistance of Beta vulgaris subsp. maritima to foliar rub-inoculation with Beet necrotic yellow vein virus

Tamada

2007

J Gen Plant Pathol

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Four lines (designated MR0, MR1, MR2, and M8) from 13 accessions of Beta vulgaris subsp. maritima were selected on the basis of phenotypes produced after foliar rub-inoculation with Beet necrotic yellow vein virus (BNYVV). The susceptible phenotype developed bright yellow local lesions, whereas the resistant phenotype had symptoms ranging from no visible lesions to necrotic lesions at the inoculation site. MR1 and MR2 lines had a resistant phenotype depending on the isolate and the MR0 line was susceptible to all isolates of BNYVV tested. The M8 line was highly susceptible; the virus spread systemically and caused severe stunting. These plant lines will be useful for distinguishing BNYVV isolates having different pathogenicities, especially those controlled by RNA3 and/or RNA5.

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“…Most of rhizomania tolerant genotypes, that perform very good in the conditions of mild infection, have one of the following types of resistance (11,29): "Rhizor", "Holly", or resistance deriving from crosses with Beta vulgaris subsp. maritima.…”

Section: Introductionmentioning

confidence: 99%

Construction of Plant Transformation Vectors Carrying Beet Necrotic Yellow Vein Virus Coat Protein Gene (II)- Plant Transformation

Nagl

Atanassov

Roussanov

et al. 2005

Biotechnology & Biotechnological Equipment

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Fragments containing the coat protein gene of beet necrotic yellow vein virus were cloned in two plant transformation vectors: pCAMBIA3301M with the bar gene as selectable marker, and pCAMBIA1304M, with resistance to hygromycin. Three constructs were made of each vector: CPL, containing coat protein gene with leader sequence; CPS with coat protein gene, and CPSas with coat protein gene in antisense orientation. Vectors pC3301MCPL, pC3301MCPS. and pC3301MCPSas were used in Agrobacteriummediated transformation of Nicotiana tabacum (tobacco), Nicotiana excelsior and Nicotiana benthamiana. Regenerants that developed roots on selective media were tested for the presence of CP fragments and the bar gene, but most regenerants were nontransformed (50-83% escapes). After all rooted plants had been selfed, and T1 seed germinated on selective media, only plants descending from one N. excelsior regenerant transformed with pC3301MCPS were positive for presence of bar gene and CPS fragment. Tobacco and Nicotiana benthamiana were transformed with constructs pC1304MCPS and pC1304MCPSas. Transformation efficiency was much higher and approximately 50% of regenerants that rooted on media with 20 mg l -1 hygromycin were positive for the presence of CP fragments. All T 1 plants were positive for presence of CP fragments.

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Identification and characterization of resistance to rhizomania in an ecotype of Beta vulgaris subsp. maritima

Cited by 14 publications

References 28 publications

Identification of amino acids of the beet necrotic yellow vein virus p25 protein required for induction of the resistance response in leaves of Beta vulgaris plants

Identification of amino acids of the beet necrotic yellow vein virus p25 protein required for induction of the resistance response in leaves of Beta vulgaris plants

Susceptibility and resistance of Beta vulgaris subsp. maritima to foliar rub-inoculation with Beet necrotic yellow vein virus

Construction of Plant Transformation Vectors Carrying Beet Necrotic Yellow Vein Virus Coat Protein Gene (II)- Plant Transformation

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