Identification and characterization of slime-producing microorganisms in papermaking water loops using emulsion polymerase chain reaction techniques

Shi, Liuxin; Su, Gang; Zhuge, Rongxia; Cheng, Qiang; Wu, Fangfang; Ni, Yonghao

doi:10.21967/jbb.v3i3.176

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“…Paper mills are an appropriate and adaptable environment for providing bacteria and fungus on development, reproduction, and nutrition (Pokhrel and Viraraghavan 2005;Roest K et al 2005;Zwieten et al 2010;Shi et al 2018;Su et al 2018;Zainith et al 2019). The present work represents the first report of Planomicrobium isolated from a paper machine along the Yangtze River in China, and it was expected to have biodegradation ability.…”

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confidence: 79%

A novel strain of Planomicrobium isolated from paper mill and its capacity of cellulose degradation

Zhuge

Cheng

Mei

et al. 2020

BioRes

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A novel strain, designated WH2-56, was isolated from a slime sample collected from a paper company along the Yangtze River during March, 2018. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain WH2-56 was related to members of the genus Planomicrobium. Cellulolytic activity of the sample was screened and confirmed by Congo red-polysaccharide interactions and examined by broth culture using filter paper (FP) with no starch as the sole carbon source. Field emission scanning electron microscopy (FE-SEM) was used to confirm the delicate morphological changes of FP during bio-degradation. Different cellulosic materials were used to measure biodegradation effects and optimum incubation conditions. The activity of FPase and carboxymethyl cellulase (CMCase) were checked by 3,5-dinitrosalicylic acid (DNS agents) with different carbon sources, which showed a peak at 0.62 U/mL of CMCase on day 4, and at 0.38 U/mL of FPase on day 5.

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