Identification and Characterization of Specific Receptor for Interleukin‐8 from the Surface of Human Monocytes

Abstract

A specific receptor for interleukin-8 has been identified on the surface of human monocytes using 125I IL-8 as a probe. A binding kinetic pattern shows that saturation was attained after 90 min and that the receptor was distinct from the receptors of other cytokines (IL-L alpha, IL-2, TNF alpha, GMCSF) and FMLP. Scatchard analysis of the binding data shows that 7000-10,000 receptors /monocyte are present with an equilibrium Kd 7 x 10(-9) M. By immunoblot, the receptor for IL-8 showed a sharp band with approximate M.W. 59 kD, consistent with the M.W. of IL-8 receptor of neutrophils. In Boyden Chamber, monocytes migrated towards IL-8 and the cytokine was observed to induce transient rise of intracellular Ca+2 in the cells. Thus, identification of functionally active IL-8 receptor in monocyte may be helpful for understanding its possible role during inflammation.

Epstein-Barr Virus Lytic Transactivator Zta Enhances Chemotactic Activity through Induction of Interleukin-8 in Nasopharyngeal Carcinoma Cells

“…However, the chemotaxis was not observed in our preliminary study (data not shown), possibly because the IL-8 concentration in the culture supernatants was too low to attract T cells which express IL-8 receptors at a lower level than neutrophils (19,30,36). IL-8 receptors have also been detected on monocytes and natural killer cells (7,43). Thus far, we have not ruled out chemotactic effects of IL-8 on infiltrating lymphocytes and other immune cells in NPC tumors in vivo.…”

A Role for TLRs in the Regulation of Immune Cell Migration by First Trimester Trophoblast Cells

“…The observation that trophoblast cells secrete high levels of IL-8 and MCP-1, which are both known to be chemotactic for monocytes (33,34), suggests that trophoblast cells may regulate monocyte migration to the maternal-fetal interface. Using an in vitro migration assay, we first sought to determine whether the levels of IL-8 and MCP-1 that were detected in the trophoblast supernatants were sufficient to induce monocyte chemotaxis and transmigration.…”

Differential expression of 9‐O‐acetylated sialoglycoconjugates on leukemic blasts: A potential tool for long‐term monitoring of children with acute lymphoblastic leukemia

“…33 Cells were incubated with increasing doses of 125 I Achatinin-H for 90 min at 4°C in the presence or absence of a 100-fold excess of unlabeled Achatinin-H. 125 I Achatinin-Hbound cells were separated from free 125 I Achatinin-H by layering the mixture onto a 5% sucrose solution followed by centrifugation at 12,857g for 10 min at 4°C. 34 Cell-bound radioactivity in the pellet was measured by a gamma counter. The number of receptors per cell was extrapolated by the intersection of the curve with the x axis, and the K d was obtained by dividing the number of receptors per cell by the bound/free ratio at the y axis intercept.…”