Identification and characterization of the Pvul-GASA gene family in the Phaseolus vulgaris and expression patterns under salt stress

Büyük, İlker; Okay, Aybüke; Górska, M; İlhan, Emre; Aras, Sümer

doi:10.3906/bot-2101-13

Cited by 7 publications

(2 citation statements)

References 59 publications

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“…On the other hand, in Phaseolus vulgaris , Pvul-GASA-18 expression is enhanced under salt stress in all tissues. However, Pvul-GASA-1 was induced in leaves and inhibited in roots, while Pvul-GASA-16/23 levels were not affected in roots but decreased in leaves under the same conditions of salt stress [ 47 ]. In Canavalia rosea , all CrGASA genes were promoted in leaves after 2 h of Mn treatment and after 48 h in roots.…”

Section: Involvement Of Gasa In Abiotic Stress Tolerance and Redox St...mentioning

confidence: 99%

GASA Proteins: Review of Their Functions in Plant Environmental Stress Tolerance

Bouteraa

Romdhane

Baazaoui

et al. 2023

Plants

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Gibberellic acid-stimulated Arabidopsis (GASA) gene family is a class of functional cysteine-rich proteins characterized by an N-terminal signal peptide and a C-terminal-conserved GASA domain with 12 invariant cysteine (Cys) residues. GASA proteins are widely distributed among plant species, and the majority of them are involved in the signal transmission of plant hormones, the regulation of plant development and growth, and the responses to different environmental constraints. To date, their action mechanisms are not completely elucidated. This review reports an overview of the diversity, structure, and subcellular localization of GASA proteins, their involvement in hormone crosstalk and redox regulation during development, and plant responses to abiotic and biotic stresses. Knowledge of this complex regulation can be a contribution to promoting multiple abiotic stress tolerance with potential agricultural applications through the engineering of genes encoding GASA proteins and the production of transgenic plants.

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Section: Involvement Of Gasa In Abiotic Stress Tolerance and Redox St...mentioning

confidence: 99%

GASA Proteins: Review of Their Functions in Plant Environmental Stress Tolerance

Bouteraa

Romdhane

Baazaoui

et al. 2023

Plants

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“…RNAseq analysis of Pvul-EIL genes was performed using SRA data developed by Hiz et al, (2014) (Büyük et al, 2019;Büyük et al, 2021c;Hiz et al, 2014;Yıldız et al, 2021). As a result, when compared to the control, certain genes were expressed at lower levels, and others at higher levels under salt and drought stress conditions (Figure 7).…”

Section: Responses Of Pvul-eil Genes To Salt and Drought Stress Throu...mentioning

confidence: 99%

EIN3/EIL (Ethylene Insensitive3 / Ethylene Insensitive3 Like) Protein Family in Phaseolus vulgaris: Identification, Evolution and Expression Analysis within the Genome

Akbulut

Şafak

Okay

et al. 2023

Turkish JAF Sci.Tech.

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Ethylene insensitive-3 (EIN3) / Ethylene insensitive-3-like (EIL) protein family is a small family of transcription factors specific to plants that play role in plant growth and development under various environmental conditions. In this study, various bioinformatics approaches were used to make an in-depth identification of the EIN3/EIL family at both the gene and protein levels. So, 11 Pvul-EIL genes were identified and their approximate locations were determined. Various biochemical and physicochemical properties of EIL proteins in Phaseolus vulgaris have been described. It was determined that Pvul-EIL proteins had a length of 447-651 amino acids and a molecular weight of 51.08-70.68 kDa. All duplications occurring in the Pvul-EIL genome were segmental type. It was observed that conserved motif, gene structure and phylogeny analyses all yielded similar results. For instance, it has been understood that genes with same motif type and number have similar gene structures and were located under the same branch in the phylogenetic tree. Pvul-EIL protein homology modeling showed that DNA binding properties and protein structure were similar to Arabidopsis EIN3. According to cis-element analysis, Pvul-EIL genes are engaged in a wide range of functions, including tissue-specific, stress, and hormone-sensitive expression. Additionally, RNAseq data was used to perform a comparative expression analysis of EIL genes. Various Pvul-EIL gene expression levels were detected under salt and drought stress. This is the first study to check the gene expression levels in P. vulgaris using in-silico detection and characterization of EIL genes. Therefore, obtained results can form the basis for future studies.

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Comprehensive identification of GASA genes in sunflower and expression profiling in response to drought

Ullah,

Ahmed,

AlHusnain

et al. 2024

BMC Genomics

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Drought stress poses a critical threat to global crop yields and sustainable agriculture. The GASA genes are recognized for their pivotal role in stress tolerance and plant growth, but little is known about how they function in sunflowers. The investigation aimed to identify and elucidate the role of HaGASA genes in conferring sunflowers with drought tolerance. Twenty-seven different HaGASA gene family members were found in this study that were inconsistently located across eleven sunflower chromosomes. Phylogeny analysis revealed that the sunflower HaGASA genes were divided into five subgroups by comparing GASA genes with those from Arabidopsis, peanut, and soybean, with members within each subgroup displaying similar conserved motifs and gene structures. In-silico evaluation of cis-regulatory elements indicated the existence of specific elements associated with stress-responsiveness being the most abundant, followed by hormone, light, and growth-responsive elements. Transcriptomic data from the NCBI database was utilized to assess the HaGASA genes expression profile in different sunflower varieties under drought conditions. The HaGASA genes expression across ten sunflower genotypes under drought stress, revealed 14 differentially expressed HaGASA genes, implying their active role in the plant’s stress response. The expression in different organs revealed that HaGASA2 , HaGASA11 , HaGASA17 , HaGASA19 , HaGASA21 and HaGASA26 displayed maximum expression in the stem. Our findings implicate HaGASA genes in mediating sunflower growth maintenance and adaptation to abiotic stress, particularly drought. The findings, taken together, provided a basic understanding of the structure and potential functions of HaGASA genes, setting the framework for further functional investigations into their roles in drought stress mitigation and crop improvement strategies. Supplementary Information The online version contains supplementary material available at 10.1186/s12864-024-10860-8.

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Identification and characterization of the Pvul-GASA gene family in the Phaseolus vulgaris and expression patterns under salt stress

Cited by 7 publications

References 59 publications

GASA Proteins: Review of Their Functions in Plant Environmental Stress Tolerance

GASA Proteins: Review of Their Functions in Plant Environmental Stress Tolerance

EIN3/EIL (Ethylene Insensitive3 / Ethylene Insensitive3 Like) Protein Family in Phaseolus vulgaris: Identification, Evolution and Expression Analysis within the Genome

Comprehensive identification of GASA genes in sunflower and expression profiling in response to drought

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