Identification and characterization of the hemolysin glycoprotein receptor on Intestine 407 cells

Detergent-Resistant Membrane Microdomains Facilitate Ib Oligomer Formation and Biological Activity of Clostridium perfringens Iota-Toxin

“…Previous investigations have shown that the Ib cell receptor consists of a protein (35). Often associated with DRMs and used by many bacterial toxins as cell receptors, GPI-anchored proteins are easily discerned by treating cells with PI-PLC, an enzyme that specifically cleaves the GPI anchor, thus releasing proteins from membranes (1,9,12,41). In order to determine whether the receptor for Ib was a GPI-anchored protein, the effects of PI-PLC treatment on Ib binding and oligomer formation were investigated.…”

“…GPI-anchored proteins, normally involved in cell signal transduction, associate with DRMs and are often used by bacterial toxins as receptors for binding to cells (9,12,41). The toxins may bind to specific GPI-anchored proteins or, as with aerolysin, bind to glycan regions conserved in several different GPI-anchored proteins.…”

Fourteen-Member Macrolides Suppress Interleukin-8 Production but Do Not Promote Apoptosis of Activated Neutrophils

“…IL-8 protein levels in culture supernatants were determined as previously described (17). Neutrophils were incubated with each reagent or with an antibiotic at 5 ϫ 10 6 cells per ml in 96-well culture plates for 48 h, as described previously, to determine cytotoxicity (23). For Northern blot analysis, neutrophils were incubated with either EM, CAM, or DEX at a concentration of 5 ϫ 10 6 cells/ml in the presence of 10% heat-inactivated autologous sera for 1 h, followed by stimulation with LPS at 1 g/ml for 30 min in flexible plates.…”

Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin

“…In this regard, uncovering the receptors present on intestinal cells is particularly important. We have previously identified p66 as the A. sobria hemolysin receptor on Intestine 407 cells (Wang et al, 1999). In the present study, we have determined that p66 is the GPIanchored ectoenzyme PLAP.…”

“…More recently, we identified a p66 hemolysin receptor protein in Intestine 407 cells, which, if identified in vivo, could serve as the possible mediator of diarrhea (Wang et al, 1999). A common characteristic of A. hydrophila and A. sobria hemolysin receptors is the fact that they are glycosylphosphatidyl inositol (GPI)-anchored.…”