Identification and characterization of the Populus sucrose synthase gene family

An, Xinmin; Zhong, Chen; Wang, Jingcheng; Ye, Meixia; Ji, Lexiang; Jia, Wang; Liao, Weihua; Ma, Huandi

doi:10.1016/j.gene.2014.01.062

Cited by 48 publications

(50 citation statements)

References 42 publications

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“…4, plant Sus genes could be divided into four sub-families. Based on several previous analyses with which our results were consistent, we here designated these sub-families as follows: Sus I eudicot group, Sus I monocot group, Sus II group, and Sus III group (An et al 2014). Most of the tobacco Sus genes belonged to the Sus I eudicot group, which included Sus genes from other eudicot species including Arabidopsis ( AtSus1 and 4 ), Gossypium arboretum ( GaSus1 , 3 , 4 , and 5 ), potato ( St2 , 3 , 4 , and 5 ), tomato ( Soly2, 3, and 4 ), pepper ( CaSus4 and 5 ) and coffee ( CoSus1 ).…”

Section: Resultsmentioning

confidence: 77%

“…Later, the Sus I group was divided into a monocot subgroup and a eudicot subgroup, as these two subgroups were obviously separate from each other in phylogenetic trees. The Sus II and Sus III groups were then considered as mix group 1 (monocot and eudicot group 1) and mix group 2, according to the categories of group members (An et al 2014). In our study, we performed phylogenetic analysis of the Sus homologs from 3 tobacco species and those of eight other plant species, and found that the tobacco Sus family had at least one gene in three separate groups.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, the maize and pea genomes contain three Sus genes (Barratt et al 2001; Duncan et al 2006), while there are six distinct Sus genes in Arabidopsis, rice, and Lotus japonicas (Baud et al 2004; Horst et al 2007; Hirose et al 2008). Seven Sus genes have been identified in poplar (An et al 2014). Diploid cotton genomes ( Gossypium arboreum L. and G. Raimondii Ulbr.)…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns

Zhong

Pan

et al. 2015

Planta

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Main conclusionProvide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco and will be beneficial for further investigations of Sus gene functionsSucrose synthase (Sus) has been well characterized as the key enzyme participating in sucrose metabolism, and the gene family encoding different Sus isozymes has been cloned and characterized in several plant species. However, scant information about this gene family is available to date in tobacco. Here, we identified 14, 6, and 7 Sus genes in the genomes of Nicotiana tabacum, N.sylvestris and N.tomentosiformis, respectively. These tobacco Sus family members shared high levels of similarity in their nucleotide and amino acid sequences. Phylogenetic analysis revealed distinct evolutionary paths for the tobacco Sus genes. Sus1–4, Sus5, and Sus6–7 originated from three Sus precursors, respectively, which were generated by duplication before the split of monocots and eudicots. There were two additional duplications, before and after the differentiation of the Solanaceae, which separately gave rise to Sus3/4 and Sus1/2. Gene exon/intron structure analysis showed that the tobacco Sus genes contain varying numbers of conserved introns, resulting from intron loss under different selection pressures during the course of evolution. The expression patterns of the NtSus genes differed from each other in various tobacco tissues. Transcripts of Ntab0259170 and Ntab0259180 were detected in leaves at all tested developmental stages, suggesting that these two genes play a predominant role in sucrose metabolism during leaf development. Expression of Ntab0288750 and Ntab0234340 were conspicuously induced by low temperature and virus treatment, indicating that these two isozymes are important in meeting the increased glycolytic demand that occurs during abiotic stress. Our results provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco, and will be beneficial for further investigations of Sus gene functions in the processes of tobacco leaf development and tobacco resistance to environmental stresses.Electronic supplementary materialThe online version of this article (doi:10.1007/s00425-015-2297-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns

Zhong

Pan

et al. 2015

Planta

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“…For instance, the Sus family is comprised of six distinct members in the model plant Arabidopsis thaliana [11], Oryza sativa [12], Gossypium arboreum [13] and Hevea brasiliensis [8]. In addition, four Sus genes were found in Hordeum vulgare [14], whereas seven Sus genes were found in the genus Populus [15], [16]. In all cases, divergent expression patterns were examined in different isozymes of the respective Sus gene families, which implied that each member of Sus gene families has a particular function in a given tissue or organ of the species.…”

Section: Introductionmentioning

confidence: 99%

“…The regulation of sugar accumulation in fruits is very important for fruit quality improvement and drought tolerance [17], [18]. Although Sus has shown its potential roles in sugar accumulation and drought tolerance through the analysis of Sus enzyme activities [3], [5], [10] and each member of Sus gene families may have a specific function in a given tissue or organ of the species [11], [13], [14], [15], [16], [19], the possible roles of citrus Sus genes are still unclear.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Profile Analysis of Citrus Sucrose Synthase Genes: Investigation of Possible Roles in the Regulation of Sugar Accumulation

Islam

Jin

et al. 2014

PLoS ONE

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Sucrose synthase (Sus) (EC 2.4.1.13) is a key enzyme for the sugar accumulation that is critical to form fruit quality. In this study, extensive data-mining and PCR amplification confirmed that there are at least six Sus genes (CitSus1-6) in the citrus genome. Gene structure and phylogeny analysis showed an evolutionary consistency with other plant species. The six Sus genes contain 12–15 exons and 11–14 introns and were evenly distributed into the three plant Sus groups (CitSus1 and CitSus2 in the Sus I group, CitSus3 and CitSus6 in the Sus II group, and CitSus4 and CitSus5 in the Sus III group). Transcripts of these six CitSus genes were subsequently examined. For tissues and organs, CitSus1 and 2 were predominantly expressed in fruit juice sacs (JS) whereas CitSus3 and 4 were predominantly expressed in early leaves (immature leaves), and CitSus5 and 6 were predominantly expressed in fruit JS and in mature leaves. During fruit development, CitSus5 transcript increased significantly and CitSus6 transcript decreased significantly in fruit JS. In the fruit segment membrane (SM), the transcript levels of CitSus2 and 5 were markedly higher and the abundant levels of CitSus3 and 6 gradually decreased. Moreover, transcript levels of CitSus1-4 examined were higher and the CitSus5 transcript level was lower in the fruit SM than in fruit JS, while CitSus6 had a similar transcript level in fruit JS and SM. In addition, transcripts of CitSus1-6 responded differently to dehydration in mature leaves or to mild drought stress in fruit JS and SM. Finally, the possible roles of Sus genes in the regulation of sugar accumulation are discussed; however, further study is required.

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Enhancement of polysaccharides accumulation in Dendrobium officinale by exogenously applied methyl jasmonate

Yuan¹,

Zhang²,

Liu³

2017

Biologia plant.

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The accumulation of polysaccharides, activities of sucrose metabolism enzymes, and the expression of sucrose biosynthetic genes in Dendrobium officinale were significantly affected by exogenous methyl jasmonate (MeJA). Application of MeJA increased the content of polysaccharides and the highest polysaccharide production occurred in the samples treated with 200 μM MeJa. The MeJA application influenced polysaccharide biosynthesis rather than degradation because the activities of sucrose metabolism enzymes and the expressions of sucrose biosynthetic genes were upregulated by MeJA. Interestingly, low MeJA concentrations promoted accumulation of Dendrobium polysaccharides, while high MeJA amounts played an inhibitory role. The content of major constituent of polysaccharides, glucose and mannose, also increased after MeJa treatment.

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Identification and characterization of the Populus sucrose synthase gene family

Cited by 48 publications

References 42 publications

Analysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns

Analysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns

Genome-Wide Identification and Expression Profile Analysis of Citrus Sucrose Synthase Genes: Investigation of Possible Roles in the Regulation of Sugar Accumulation

Enhancement of polysaccharides accumulation in Dendrobium officinale by exogenously applied methyl jasmonate

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