Identification and Characterization of the Pathogen Causing Canker Disease in Acid Lime (Citrus aurantiifolia) cv. Petlur Acid Lime

Abstract

In this study, the pathogen responsible for canker disease in Acid Lime Cv. Petlur Acid Lime was identified through a comprehensive analysis involving both morphological and molecular characterization techniques. Cultures of the Xanthomonas campestris pv. citri (Xcc) bacterial isolate, grown on artificial media, exhibited distinct characteristics: small, round, and mucoid colonies with a gram-negative nature, as revealed by Gram staining. Molecular identification was performed using universal primers 27F and 1492R, resulting in a specific DNA band of 424 bp, confirming the presence of the pathogen in the samples.