Identification and characterization of the melanoma differentiation – associated gene 5 in sea perch, Lateolabrax japonicus

Jia, Peng; Jia, Kuntong; Chen, Limin; Le, Yi; Jin, Yilin; Zhang, Jing; Zhu, Limei; Zhang, Li; Yi, Meisheng

doi:10.1016/j.dci.2016.03.029

Cited by 29 publications

(19 citation statements)

References 37 publications

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“…For example, zebrafish RIG-I plays an essential role in group II type I IFN induction during NNV infection [6]. Our previous studies also suggested that the RLRs signaling pathway is activated during red spotted grouper nervous necrosis virus (RGNNV) infection in sea perch (Lateolabrax japonicus) and its key components possessed anti-RGNNV activities [7,8]. It has been known that viruses can escape from host recognition by degradation of RLRs or interference with the RLRs signaling to establish persistent infections [9].…”

Section: Introductionmentioning

confidence: 99%

MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination

Liu

Jin

Zhang

et al. 2020

Viruses

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The RIG-I-like receptors (RLRs) signaling pathway is essential for inducing type I interferon (IFN) responses to viral infections. Meanwhile, it is also tightly regulated to prevent uncontrolled immune responses. Numerous studies have shown that microRNAs (miRNAs) are essential for the regulation of immune processes, however, the detailed molecular mechanism of miRNA regulating the RLRs signaling pathway remains to be elucidated. Here, our results showed that miR-202-5p was induced by red spotted grouper nervous necrosis virus (RGNNV) infection in zebrafish. Overexpression of miR-202-5p led to reduced expression of IFN 1 and its downstream antiviral genes, thus facilitating viral replication in vitro. In comparison, significantly enhanced levels of IFN 1 and antiviral genes and significantly low viral burden were observed in the miR-202-5p-/- zebrafish compared to wild type zebrafish. Subsequently, zebrafish tripartite motif-containing protein 25 (zbTRIM25) was identified as a target of miR-202-5p in both zebrafish and humans. Ectopic expression of miR-202-5p suppressed zbTRIM25-mediated RLRs signaling pathway. Furthermore, we showed that miR-202-5p inhibited zbTRIM25-mediated zbRIG-I ubiquitination and activation of IFN production. In conclusion, we demonstrate that RGNNV-inducible miR-202-5p acts as a negative regulator of zbRIG-I-triggered antiviral innate response by targeting zbTRIM25. Our study reveals a novel mechanism for the evasion of the innate immune response controlled by RGNNV.

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Section: Introductionmentioning

confidence: 99%

MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination

Liu

Jin

Zhang

et al. 2020

Viruses

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“…Similarly, induction of MDA5 and LGP2 was observed in vitro in LPS‐stimulated peripheral blood leucocytes and leucocytes from kidney of Japanese flounder, In grass carp, RIG‐I, MDA5 and LGP2 were all up‐regulated in primary trunk kidney cells following LPS exposure . However, in sea perch, a decrease in the expression of MDA5 was observed in LPS‐stimulated brain and fry cells, and it was assumed that signal pathways mediated by TLRs or NLRs were likely to be inhibited following the expression of MDA5 . To our knowledge, the mechanisms involved in the LPS recognition and signalling transduction remain unknown in fish, although TLR4 orthologues are reported in some teleost fish (not all teleosts) such as in zebrafish.…”

Section: In Vivo and In Vitro Expression Of Rlrs In Teleost Fishmentioning

confidence: 70%

“…Currently, RIG‐I gene is found only in Cypriniformes, Siluriformes and Salmoniformes, whereas both MDA5 and LGP2 genes are found in fish species belonging to Cypriniformes, Siluriformes, Salmoniformes and other fish in Acanthopterygii (Table ) . It is uncertain whether RIG‐I genes have been lost in fish of Acanthopterygii, which requires certainly further research on this group of fish.…”

Section: Findings Of Rlrs and Their Spliced Variants In Teleost Fishmentioning

confidence: 99%

“…Comparatively, RLRs in fish contain protein domains similar to those of mammalian RLRs. All RLR molecules comprise a DExD/H box helicase domain (DEXDc), a helicase C‐terminal domain (HELICc), a regulatory domain (RD), two caspase activation and recruitment domains (CARDs) at the N‐terminal region of RIG‐I and MDA5, but not LGP2, with CARD functioning critically in signalling transduction …”

Section: Findings Of Rlrs and Their Spliced Variants In Teleost Fishmentioning

confidence: 99%

“…Infection of channel catfish virus enhanced the expression level of RIG‐I, MDA5 and LGP2 in channel catfish ( Ictalurus punctatus ) . RLRs in perciform fish, such as MDA5 in orange spotted grouper ( Epinephelus coioides ) and green chromide ( Etroplus suratensis ), MDA5 and LGP2 in large yellow croaker ( Larimichthys crocea ) and sea perch ( Lateolabrax japonicas ), had increased transcripts following the treatment of poly(I:C) and infection of DNA or RNA viruses, such as VHSV, Singapore grouper iridovirus and nervous necrosis virus . In addition, the up‐regulation of MDA5 and LGP2 mRNA levels was detected in the kidney of VHSV‐infected Japanese flounder and in vitro in poly(I:C) stimulated whole kidney leucocytes …”

Section: In Vivo and In Vitro Expression Of Rlrs In Teleost Fishmentioning

confidence: 99%

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Retinoic acid‐inducible gene I (RIG‐I)‐like receptors (RLRs) in fish: current knowledge and future perspectives

2017

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Summary Retinoic acid‐inducible gene I (RIG‐I) ‐like receptors (RLRs) are found conservatively present in teleost fish. All three members, RIG‐I, MDA5 and LGP2, together with the downstream molecules such as MITA, TRAF3 and TBK1, have been identified in a range of fish species. However, it is unexpected that RIG‐I has not been reported in fish of Acanthopterygii, and it would be important to clarify the presence and role of the RIG‐I gene in a broad range of taxa in Teleostei. RLRs in fish can be induced in vivo and in vitro by viral pathogens as well as synthetic dsRNA, poly(I:C), leading to the production of type I interferons (IFNs) and the expression of IFN‐stimulated genes (ISGs). Bacterial pathogens, such as Edwardsiella tarda, and their components, such as lipopolysaccharide are also found to induce the expression of RLRs, and whether such induction was mediated through the direct recognition by RLRs or through crosstalk with other pattern recognition receptors recognizing directly bacterial pathogen‐associated molecular patterns awaits to be investigated. On the other hand, RLR‐activated type I IFN production can be negatively regulated in fish by molecules, such as TBK‐1‐like protein and IRF10, which are found to negatively regulate RIG‐I and MAVS‐activated type I IFN production, and to block MITA or bind ISRE motifs, respectively. It is considered that the evolutionary occurrence of RLRs in fish, and their recognized ligands, especially those from their fish pathogens, as well as the mechanisms involved in the RLR signalling pathways, are of significant interest for further investigation.

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Molecular characterization and expression analysis of two RING-between-RING (RBR) ubiquitin ligase orthologues from the Asian seabass (Lates calcarifer)

2022

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Identification and characterization of the melanoma differentiation – associated gene 5 in sea perch, Lateolabrax japonicus

Cited by 29 publications

References 37 publications

MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination

MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination

Retinoic acid‐inducible gene I (RIG‐I)‐like receptors (RLRs) in fish: current knowledge and future perspectives

Molecular characterization and expression analysis of two RING-between-RING (RBR) ubiquitin ligase orthologues from the Asian seabass (Lates calcarifer)

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