Identification and characterization of the BmCyclin L1-BmCDK11A/B complex in relation to cell cycle regulation

Liu, Tai-Hang; Wu, Yunfei; Dong, Xinxin; Pan, Cai-Xia; Du, Guo-yu; Yang, Ji-gui; Wang, Wei; Bao, Xiaoyong; Chen, Peng; Pan, Min‐Hui; Lü, Cheng

doi:10.1080/15384101.2017.1304339

Cited by 12 publications

(9 citation statements)

References 35 publications

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“…Consistently, previously studies have reported the tumor-promoting anti-cancer effects of CDK11A (30,31). Liu and colleagues also found that CDK11A upregulation suppresses cellular proliferation by inducing cell cycle arrest (32).…”

Section: Discussionsupporting

confidence: 80%

Identification of Mutator-Derived Alternative Splicing Signatures of Genomic Instability for Improving the Clinical Outcome of Cholangiocarcinoma

et al. 2021

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BackgroundCholangiocarcinoma is an aggressive carcinoma with increasing incidence and poor outcomes worldwide. Genomic instability and alternative splicing (AS) events are hallmarks of carcinoma development and progression. The relationship between genomic instability, AS events, and tumor immune microenvironment remain unclear.MethodsThe splicing profiles of patients with cholangiocarcinoma were obtained from The Cancer Genome Atlas (TCGA) spliceSeq database. The transcriptomics, simple nucleotide variation (SNP) and clinical data of patients with cholangiocarcinoma were obtained from TCGA database. Patients were divided into genomic unstable (GU-like) and genomic stable (GS-like) groups according to their somatic mutations. Survival-related differential AS events were identified through integrated analysis of splicing profiling and clinical data. Kyoto Encyclopedia of Genes and Genomes enrichment analysis was used to identify AS events occurring in genes enriched in cancer pathways. Pearson correlation was applied to analyze the splicing factors regulating AS events. CIBERSORT was used identify differentially infiltrating immune cells.ResultsA prognostic signature was constructed with six AS events. Using this signature, the hazard ratio of risk score for overall survival is 2.362. For TCGA patients with cholangiocarcinoma, the area under the receiver operating characteristic curve is 0.981. CDK11A is a negative regulator of survival associated AS events. Additionally, the CD8+ T cell proportion and PD-L1 expression are upregulated in patients with cholangiocarcinoma and high splicing signatures.ConclusionWe provide a prognostic signature for cholangiocarcinoma overall survival. The CDK11A splicing factor and SLC46A1-39899-ES and IARS-86836-ES AS events may be potential targets for cholangiocarcinoma therapy. Patients with high AS risk score may be more sensitive to anti-PD-L1/PD1 immunotherapy.

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Section: Discussionsupporting

confidence: 80%

Identification of Mutator-Derived Alternative Splicing Signatures of Genomic Instability for Improving the Clinical Outcome of Cholangiocarcinoma

et al. 2021

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“…Cytosolic phospholipase has been verified to function in the synthesis of prostanoid involved in ovarian development in Penaeus monodon 30 . In addition, prediction results showed that many target genes participate in gonadal development, such as cyclin L1, HSP, and zinc finger protein 31–33 . For instance, Hsp110 is required for progression of spermatogenesis, and is associated with germ cell apoptosis 32 .…”

Section: Discussionmentioning

confidence: 99%

Identification and profiling of microRNAs during gonadal development in the giant freshwater prawn Macrobrachium rosenbergii

Luo

Feng

et al. 2019

Sci Rep

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As post-transcriptional regulators, microRNAs (miRNAs) play an important role in growth and reproductive processes. So far, there is limited information regarding crustacean miRNAs. To explore the potential role of miRNAs in the gonadal development of the prawn Macrobrachium rosenbergii, we constructed seven small RNA libraries from ovarian and testicular tissues at various stages using somatic tissue as the control. A total of 1,954 known and 129 novel miRNAs were retrieved. By comparing differentially expressed miRNAs (DEMs) between testes and ovaries, forty-one miRNAs were identified with sex-biased expression patterns, including 17 ovary-biased and 24 testis-biased patterns. Furthermore, the putative target genes of the sex-biased miRNAs, such as cyclin L1, mitogen-activated protein kinase 7 (MAPK 7), heat shock protein (HSP), and zinc finger protein, were significantly enriched in many reproduction-related pathways including the Gonadotropin-releasing hormone (GnRH) pathway, glycolysis, gluconeogenesis pathway, ovarian steroidogenesis, estrogen signaling pathway, MAPK pathway, Wnt pathway, and insulin signaling pathway, implicating potential regulatory roles of miRNAs in reproduction. These data aid in the further investigation of the mechanism of gonadal development and reproductive regulation mediated by miRNA in M. rosenbergii.

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“…Conversely, blocking CDK11 and/or Cyclin L1 expression using siRNAs arrested these cells in G2/M, with decreased G1 phase cells. Both overexpression and inhibited expression of CDK11 and/or Cyclin L1 reduced the viability of these cells, suggesting a window of appropriate CDK11 levels in these non-cancer cells [57]. The effects of CDK11 loss in silkworm cells align with similar effects during mouse embryo development [15].…”

Section: Discussionmentioning

confidence: 99%

CDK11 Loss Induces Cell Cycle Dysfunction and Death of BRAF and NRAS Melanoma Cells

et al. 2019

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Cyclin dependent kinase 11 (CDK11) is a protein kinase that regulates RNA transcription, pre-mRNA splicing, mitosis, and cell death. Targeting of CDK11 expression levels is effective in the experimental treatment of breast and other cancers, but these data are lacking in melanoma. To understand CDK11 function in melanoma, we evaluated protein and RNA levels of CDK11, Cyclin L1 and Cyclin L2 in benign melanocytes and BRAF- as well as NRAS-mutant melanoma cell lines. We investigated the effectiveness of reducing expression of this survival kinase using RNA interference on viability, clonal survival, and tumorsphere formation in melanoma cell lines. We examined the impact of CDK11 loss in BRAF-mutant melanoma on more than 700 genes important in cancer signaling pathways. Follow-up analysis evaluated how CDK11 loss alters cell cycle function in BRAF- and NRAS-mutant melanoma cells. We present data on CDK11, CCNL1 and CCNL2 mRNA expression in melanoma patients, including prognosis for survival. In sum, we found that CDK11 is necessary for melanoma cell survival, and a major impact of CDK11 loss in melanoma is to cause disruption of the cell cycle distribution with accumulation of G1- and loss of G2/M-phase cancer cells.

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Identification and characterization of the BmCyclin L1-BmCDK11A/B complex in relation to cell cycle regulation

Cited by 12 publications

References 35 publications

Identification of Mutator-Derived Alternative Splicing Signatures of Genomic Instability for Improving the Clinical Outcome of Cholangiocarcinoma

Identification of Mutator-Derived Alternative Splicing Signatures of Genomic Instability for Improving the Clinical Outcome of Cholangiocarcinoma

Identification and profiling of microRNAs during gonadal development in the giant freshwater prawn Macrobrachium rosenbergii

CDK11 Loss Induces Cell Cycle Dysfunction and Death of BRAF and NRAS Melanoma Cells

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