Identification and characterization of the diuretic hormone 31 receptor in the silkwormBombyx mori

Abstract

The receptor for diuretic hormone 31 (DH31R) was identified in the silkworm Bombyx mori. A heterologous expression system revealed that an orphan G-protein coupled receptor, BNGR-B1, responded to DH31 and upregulated the intracellular cAMP level. DH31R (BNGR-B1) was predominantly expressed in the anterior silk gland, midgut, and ovary, whereas DH31 was predominantly expressed in the central nervous system and midgut.

Characterization of an evolutionarily conserved calcitonin signaling system in a lophotrochozoan, the Pacific oyster (Crassostrea gigas)

“…This suggests the existence of multiple conformational states for the oyster receptors, with distinct pharmacological and signalling properties. Dual activation was also observed for vertebrate CT (Force et al, 1992), although cAMP signalling represents the most common pathway to mediate the activity of the CT/CGRP/DH31 peptide family (Gorn et al, 1992;Coast et al, 2001;Iga and Kataoka, 2015).…”

“…Dippu-DH31 shows a small amount of sequence similarity with the CT family of peptides, but lacks the typical N-terminal pair of cysteine residues. As insect DH31 homologs specifically activate CTR/CLR-related receptors (Iga and Kataoka, 2015;Johnson, 2004;Zandawala et al, 2013), they were initially considered as the insect evolutionary counterparts of the deuterostome CT/CGRP family of peptides. However, recent mining of the termite and locust genomes revealed the existence of a gene encoding typical CT-related peptides with an N-terminal disulfide bridge in addition to the DH31-encoding genes present in all insect genomes (Veenstra, 2014).…”

Multiple neuropeptides produced by sex-specific neurons control activity of the male accessory glands and gonoducts in the silkworm Bombyx mori

“…Since neuropeptides produced by MAN9 modulated myoactivity of gonoducts in vitro , we next examined presence of their receptors in reproductive organs. These G-protein coupled receptors have been previously characterized 18–20 . Quantitative RT-PCR revealed that receptors for CT-DH (BNGR-B1), AT (BNGR-A16), AST-C (BNGR-A1) and MIP (sex peptide receptor) are all expressed in reproductive tissues innervated by MAN9.…”

Functional Identification and Characterization of the Diuretic Hormone 31 (DH31) Signaling System in the Green Shore Crab, Carcinus maenas

“… DH31R amino acid alignments of C. maenas DH31R (Acc No: MH331892) compared with other functionally deorphanized insect homologs: Rhodnius prolixus DH31R2B, DH31R1B and DH31R1C KF446640, KC660149, KC660150; ( Zandawala et al, 2013 ); Bombyx mori DH31R, NP_001127732 ( Iga and Kataoka, 2015 ); Aedes aegypti DH31R, JQ045343; ( Kwon and Pietrantonio, 2013 ); and Drosophila melanogaster DH31R NM_165979 ( Johnson et al, 2005 ). For ease of interpretation, overall comparative amino acid identities are indicated by black (100%), dark gray (80–99%), light gray (60–79%) and white (less than 60%).…”

“…With regard to DH31 receptors, a number of these have been predicted in insects from protein BLAST searches (see Caers et al, 2012 for list), but relatively few have been functionally confirmed using heterologous cell-based reporting systems. Examples are Drosophila ( Johnson et al, 2005 ), Rhodnius prolixus ( Zandawala et al, 2013 ), Bombyx mori ( Iga and Kataoka, 2015 ), or by RNAi and immunochemical visualization in Aedes aegypti ( Kwon and Pietrantonio, 2013 ). Although several putative crustacean neuropeptide receptors, including that of DH31 have been proposed from de novo transcriptome assemblies in C. finmarchicus and H. americanus ( Christie et al, 2013 , 2017 ), to date very few of these have been functionally confirmed in crustaceans, which is a mandatory step in unequivocal receptor identification.…”