Identification and characterization of the major proteins of malignant catarrhal fever virus

Shen, David; Davis, William C.; Knowles, Donald P.; Gorham, J. R.; Crawford, Timothy B.

doi:10.1099/0022-1317-76-1-123

Cited by 25 publications

(19 citation statements)

References 14 publications

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“…The hemi‐nested PCR used in this study is considered by many to be the best molecular PCR assay for the diagnosis of SA‐MCF in clinical samples (Li et al., ; Muller‐Doblies et al., ). Bovine blood samples were negative for the hemi‐nested PCR, indicating that the viraemia stage was not present at the time of sample collection, but ovine blood samples were still showing the viral DNA in the PCR.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Histopathological Characterization of Sheep-Associated Malignant Catarrhal Fever (SA-MCF) Outbreak in Beef Cattle

Ababneh

Hananeh

Dalab

2012

Transbound Emerg Dis

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An outbreak of suspected malignant catarrhal fever (MCF) was investigated by molecular and histopathological assays. Of the 70 Holstein beef calf herds, 14 were affected by multiple clinical signs suggestive of MCF infection. These beef calves were housed next to sheep flocks. In the complete blood count, the 14 affected calves had severe anaemia with leucopaenia, lymphopaenia and neutropaenia. Upon PCR amplification using a hemi-nested PCR assay for the detection of the Ovine herpesvirus 2 (OvHV-2), bovine tissue samples from the mesenteric lymph nodes and spleen and ovine blood samples were shown to be positive with the expected PCR bands amplified. Direct sequencing of the hemi-nested PCR product confirmed the identity of the causative virus as OvHV-2. The histopathological findings confirmed the clinical and laboratory diagnosis of MCF. Collective clinical, PCR and histopathological data confirmed the identity of this outbreak to be a sheep-associated malignant catarrhal fever (SA-MCF).

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Section: Discussionmentioning

confidence: 99%

Molecular and Histopathological Characterization of Sheep-Associated Malignant Catarrhal Fever (SA-MCF) Outbreak in Beef Cattle

Ababneh

Hananeh

Dalab

2012

Transbound Emerg Dis

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“…Antibody against the 15A epitope, 12,14 which is shared among all known strains of MCF viruses, has been demonstrated in a variety of wild ruminant species. 13 The impact of MCF virus on free-ranging populations of ruminants such as the moose 25 and a variety of cervid species 21 is unknown.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Malignant Catarrhal Fever by PCR Using Formalin-Fixed, Paraffin-Embedded Tissues

Crawford

O’Toole

1999

J VET Diagn Invest

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Abstract.A previously described polymerase chain reaction (PCR) assay (amplification of a 238-bp fragment of ovine herpesvirus 2 [OHV-2] genomic DNA) for diagnosis of sheep-associated malignant catarrhal fever (MCF) was adapted for use on formalin-fixed, paraffin-embedded tissues. Variables affecting its use were examined. Archived tissues from cattle, white-tailed deer (Odocoileus virginianus), and bison (Bison bison) diagnosed with MCF by clinical signs or histologic lesions were obtained from 2 veterinary diagnostic laboratories. Tissues from healthy animals and from animals diagnosed with other common bovine viral diseases were examined as controls. A total of 86 blocks from 37 suspect MCF cases were examined. Forty-one blocks from healthy animals and animals with unrelated viral diseases were examined as controls. The assay was specific for sheep-associated MCF and did not yield false-positive signals from healthy animals or from cases of infectious bovine rhinotracheitis, bovine virus diarrhea, mucosal disease, or parainfluenza-3 virus infection. A wide variety of tissues were suitable substrates, including spleen, lymph node, intestine, brain, lung, and kidney. Extracted DNA provided a more suitable target than did unextracted tissue lysate. The highest levels of viral DNA were present in lymphoid organs and intestine, but the data indicate that in acute clinical cases, most organs contain sufficient viral DNA to serve as a suitable diagnostic specimen. Fixation of 0.5-cm 3 blocks of tissue in 10% neutral buffered formalin was deleterious to the target DNA, and PCR signals progressively diminished after fixation for Ͼ45 days. Detection of genomic DNA of OHV-2 by PCR was successful for archived tissues that were 15 years old.

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“…Polyclonal antibody-based assays, including ELISA, IFA, and IPT among others, detect antibodies against multiple epitopes of AlHV-1. Generally, these tests have good sensitivity, but reduced specificity, due to cross-reactivity with other herpesviruses, such as bovine herpesviruses 1 and 4 [63,64]. To increase specificity, a cELISA for detection of MCF viral antibody was developed using an antibody (15-A) against an epitope conserved among all MCFVs examined to date [58].…”

Section: Serological Testsmentioning

confidence: 99%

“…The specificity for OvHV-2 arises from one of the primers (#556), which binds to a region of low homology between OvHV-2 and AlHV-1 [70]. This nested PCR has high sensitivity and is validated for detection of OvHV-2 DNA in infected sheep as well as in animals with clinical MCF [64,78]. The assay has been widely used in veterinary diagnostic laboratories; however, its use as a routine method to detect OvHV-2 DNA for confirmation of clinical SA-MCF in diagnostic laboratories may be problematic due to a high potential for amplicon contamination leading to false positive results in diagnostic laboratories.…”

Section: Polymerase Chain Reaction (Pcr) Assaysmentioning

confidence: 99%

Malignant Catarrhal Fever: Understanding Molecular Diagnostics in Context of Epidemiology

Cunha

Taus

2011

IJMS

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Malignant catarrhal fever (MCF) is a frequently fatal disease, primarily of ruminants, caused by a group of gammaherpesviruses. Due to complexities of pathogenesis and epidemiology in various species, which are either clinically-susceptible or reservoir hosts, veterinary clinicians face significant challenges in laboratory diagnostics. The recent development of specific assays for viral DNA and antibodies has expanded and improved the inventory of laboratory tests and opened new opportunities for use of MCF diagnostics. Issues related to understanding and implementing appropriate assays for specific diagnostic needs must be addressed in order to take advantage of molecular diagnostics in the laboratory.

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Identification and characterization of the major proteins of malignant catarrhal fever virus

Cited by 25 publications

References 14 publications

Molecular and Histopathological Characterization of Sheep-Associated Malignant Catarrhal Fever (SA-MCF) Outbreak in Beef Cattle

Molecular and Histopathological Characterization of Sheep-Associated Malignant Catarrhal Fever (SA-MCF) Outbreak in Beef Cattle

Diagnosis of Malignant Catarrhal Fever by PCR Using Formalin-Fixed, Paraffin-Embedded Tissues

Malignant Catarrhal Fever: Understanding Molecular Diagnostics in Context of Epidemiology

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