Identification and characterization of the biotechnological potential of a wild strain of Paraconiothyrium sp

Abstract

The isolation and characterization of fungal strains from poorly described taxa allows undercover attributes of their basic biology useful for biotechnology. Here, a wild fungal strain (CMU-196) from recently described Paraconiothyrium genus was analyzed. CMU-196 was identified as Paraconiothyrium brasiliense by phylogenetic analysis of the rDNA internal transcribed spacer region (ITS). CMU-196 metabolized 57 out of 95 substrates of the Biolog FF microplates. Efficient assimilation of dextrins and glycogen indicates that CMU-196 is a good producer of amylolytic enzymes. It showed a remarkably assimilation of α-d-lactose, substrate described as inducer of cellulolytic activity but poorly assimilated by several fungi. Metabolically active mycelium of the strain decolorized broth supplemented with direct blue 71, Chicago sky blue and remazol brilliant blue R dyes. The former two dyes were also well removed from broth by mycelium inactivated by autoclaving. Both mycelia had low efficiency for removing fuchsin acid from broth and for decolorizing wastewater from the paper industry. CMU-196 strain showed extracellular laccase activity when potato dextrose broth was supplemented with Cu , reaching a maximum activity of 46.8 (±0.33) U L . Studied strain antagonized phytopathogenic Colletotrichum spp. fungi and Phytophthora spp. oomycetes in vitro, but is less effective towards Fusarium spp. fungi. CMU-196 antagonism includes overgrowing the mycelia of phytopathogens and growth inhibition, probably by hydrosoluble extracellular metabolites. The biotechnological potential of strain CMU-196 here described warrants further studies to have a more detailed knowledge of the mechanisms associated with its metabolic versatility, capacity for environmental detoxification, extracellular laccase production, and antagonism against phytopathogens. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:846-857, 2018.

Metabolomics analysis of mycelial exudates provides insights into fungal antagonists of Armillaria

“…Moreover, metabolites like lignans, neolignans, and polyketides were identified in our study, but were rarely identified from the mycelia and fruiting bodies in previous studies (Muszynska et al 2011 ; Zhang et al 2015 ; Li et al 2016 ; Ren et al 2022 ), showing the metabolic differences between mycelial exudates, mycelia and fruiting bodies. Furthermore, compared to the separate culture modes, polyketides were generally repressed in C-G co-culture, which might be due to metabolic changes in response to biotic stimulus in the co-culture of Armillaria species with antagonism effect (Nicoletti et al 2004 ; Arredondo-Santoyo et al 2018 ; Dullah et al 2021 ). Lignans and neolignans, which were newly identified in our study, had significant bioactivities: lignans could inhibit the formation and growth of hormone-dependent cancer cells and protect the human body against oestrogen-related diseases, such as osteoporosis and breast cancer (Ionkova 2011 ).…”

Whole-genome sequencing and functional annotation of pathogenic Paraconiothyrium brasiliense causing human cellulitis

“…For instance, P. brasiliense isolated from Himalayan yew produces paclitaxel, with antitumor activity [ 9 ]. Arredondo-Santoyo et al [ 10 ] showed that P. brasiliense expresses an activity antagonistic to Colletotrichum spp. and Phytophthora spp.…”