Identification and Characterization of the Duplicate Rice Sucrose Synthase Genes OsSUS5 and OsSUS7 Which Are Associated with the Plasma Membrane

Cho, Jung‐Il; Kim, Hyun-Bi; Kim, Chi-Yeol; Hahn, Tae‐Ryong; Jeon, Jong‐Seong

doi:10.1007/s10059-011-1038-y

Cited by 28 publications

(24 citation statements)

References 73 publications

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“…In this regard, transcripts encoding SUS (Os03t0401300, SUS1 ; Os04t0249500, OsSUS7 ; Os03t0340500, SUS4 ) were up-regulated in Xieyou 9308 compared with the parental lines. As reported previously, OsSUS7 is highly expressed in roots [39], and SUS1 is predominantly expressed in elongating tissues, including roots where rapid formation of secondary wall takes place just after cell elongation [40]. The high level of SUS expression in Xieyou 9308 may play a major role in sink organs by providing carbon sources for sink metabolism and SUS expression may promote root elongation.…”

Section: Discussionmentioning

confidence: 74%

Transcriptome analysis of rice root heterosis by RNA-Seq

et al. 2013

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BackgroundHeterosis is a phenomenon in which hybrids exhibit superior performance relative to parental phenotypes. In addition to the heterosis of above-ground agronomic traits on which most existing studies have focused, root heterosis is also an indispensable component of heterosis in the entire plant and of major importance to plant breeding. Consequently, systematic investigations of root heterosis, particularly in reproductive-stage rice, are needed. The recent advent of RNA sequencing technology (RNA-Seq) provides an opportunity to conduct in-depth transcript profiling for heterosis studies.ResultsUsing the Illumina HiSeq 2000 platform, the root transcriptomes of the super-hybrid rice variety Xieyou 9308 and its parents were analyzed at tillering and heading stages. Approximately 391 million high-quality paired-end reads (100-bp in size) were generated and aligned against the Nipponbare reference genome. We found that 38,872 of 42,081 (92.4%) annotated transcripts were represented by at least one sequence read. A total of 829 and 4186 transcripts that were differentially expressed between the hybrid and its parents (DGHP) were identified at tillering and heading stages, respectively. Out of the DGHP, 66.59% were down-regulated at the tillering stage and 64.41% were up-regulated at the heading stage. At the heading stage, the DGHP were significantly enriched in pathways related to processes such as carbohydrate metabolism and plant hormone signal transduction, with most of the key genes that are involved in the two pathways being up-regulated in the hybrid. Several significant DGHP that could be mapped to quantitative trait loci (QTLs) for yield and root traits are also involved in carbohydrate metabolism and plant hormone signal transduction pathways.ConclusionsAn extensive transcriptome dataset was obtained by RNA-Seq, giving a comprehensive overview of the root transcriptomes at tillering and heading stages in a heterotic rice cross and providing a useful resource for the rice research community. Using comparative transcriptome analysis, we detected DGHP and identified a group of potential candidate transcripts. The changes in the expression of the candidate transcripts may lay a foundation for future studies on molecular mechanisms underlying root heterosis.

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Section: Discussionmentioning

confidence: 74%

Transcriptome analysis of rice root heterosis by RNA-Seq

et al. 2013

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“…Since accumulation of other carbohydrates synthesized from the UDP-glucose precursor, such as cellulose and β-glucan, were unaffected in the mutant, we suggest that the increased expression of the genes for sucrose synthase 4 and UDPase 1 may be also associated with amylopectin synthesis. Sucrose synthase 4 is suggested to be cytosolic [23] and may produce UDP-glucose, which is converted by UDPase 1 to the hexose-phosphate used for amylopectin synthesis [16]. Indeed, sucrose synthases 2 and 3 in Arabidopsis, which belong to the same group as rice sucrose synthase 4 [23], have been recently reported to direct carbon to starch synthesis [62].…”

Section: Discussionmentioning

confidence: 99%

“…Sucrose synthase 4 is suggested to be cytosolic [23] and may produce UDP-glucose, which is converted by UDPase 1 to the hexose-phosphate used for amylopectin synthesis [16]. Indeed, sucrose synthases 2 and 3 in Arabidopsis, which belong to the same group as rice sucrose synthase 4 [23], have been recently reported to direct carbon to starch synthesis [62]. In our experiment, the elevated expression of cytosolic AGP supports that notion.…”

Section: Discussionmentioning

confidence: 99%

“…Several proteins have been implicated as important players in carbon partitioning in plants. They include proteins involved in sugar transport and metabolism, such as sucrose transporters [21], sucrose invertases [22] and sucrose synthases [23,24], and in hexose metabolism and transport, such as hexose kinases [25] and monosaccharide transporters [26]. Other examples include proteins controlling the flux in polysaccharide biosynthesis, such as ADP-glucose pyrophosphorylase [27], and UDP-glucose pyrophosphorylase [28,29], and regulatory proteins, such as sucrose non-fermenting-1-related protein kinase [30], trehalose-6-phosphate synthase [31], and transcription factors [12,32-35].…”

Section: Introductionmentioning

confidence: 99%

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Molecular insights into how a deficiency of amylose affects carbon allocation – carbohydrate and oil analyses and gene expression profiling in the seeds of a rice waxy mutant

et al. 2012

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BackgroundUnderstanding carbon partitioning in cereal seeds is of critical importance to develop cereal crops with enhanced starch yields for food security and for producing specified end-products high in amylose, β-glucan, or fructan, such as functional foods or oils for biofuel applications. Waxy mutants of cereals have a high content of amylopectin and have been well characterized. However, the allocation of carbon to other components, such as β-glucan and oils, and the regulation of the altered carbon distribution to amylopectin in a waxy mutant are poorly understood. In this study, we used a rice mutant, GM077, with a low content of amylose to gain molecular insight into how a deficiency of amylose affects carbon allocation to other end products and to amylopectin. We used carbohydrate analysis, subtractive cDNA libraries, and qPCR to identify candidate genes potentially responsible for the changes in carbon allocation in GM077 seeds.ResultsCarbohydrate analysis indicated that the content of amylose in GM077 seeds was significantly reduced, while that of amylopectin significantly rose as compared to the wild type BP034. The content of glucose, sucrose, total starch, cell-wall polysaccharides and oil were only slightly affected in the mutant as compared to the wild type. Suppression subtractive hybridization (SSH) experiments generated 116 unigenes in the mutant on the wild-type background. Among the 116 unigenes, three, AGP, ISA1 and SUSIBA2-like, were found to be directly involved in amylopectin synthesis, indicating their possible roles in redirecting carbon flux from amylose to amylopectin. A bioinformatics analysis of the putative SUSIBA2-like binding elements in the promoter regions of the upregulated genes indicated that the SUSIBA2-like transcription factor may be instrumental in promoting the carbon reallocation from amylose to amylopectin.ConclusionAnalyses of carbohydrate and oil fractions and gene expression profiling on a global scale in the rice waxy mutant GM077 revealed several candidate genes implicated in the carbon reallocation response to an amylose deficiency, including genes encoding AGPase and SUSIBA2-like. We believe that AGP and SUSIBA2 are two promising targets for classical breeding and/or transgenic plant improvement to control the carbon flux between starch and other components in cereal seeds.

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“…The identification of the SUS genes is the starting point towards revealing their physiological roles. Presently, a number of SUS gene families have been identified in many plant species [14][15][16][17][18][19]. These studies have demonstrated that SUS genes occur as isoforms and are encoded by a small multi-gene family.…”

Section: Introductionmentioning

confidence: 99%