Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey

Adıgüzel, Ahmet; Özkan, Hakan; Barış, Özlem; İnan, Kadriye; Güllüce, Medine; Şahın, Fikrettin

doi:10.1016/j.mimet.2009.09.026

Cited by 106 publications

(65 citation statements)

References 45 publications

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“…This observation is valid even for phylotypes represented by four or less strains (Tables 3 and 4). This finding demonstrates that these phylotypes contain considerable genetic variation despite their high (≥ 99%) 16S rDNA sequence similarity, which is in agreement with previous reports (Adiguzel et al, 2009;Papalexandratou et al, 2009). This explains why we failed to cluster isolates belonging to the same phylotype using (GTG) 5 -PCR and BOXA1R-PCR fingerprinting although it has been reported that rep-PCR has a higher taxonomic resolution than 16S rRNA gene sequencing (Ishii and Sadowsky, 2009 (1 isolate) were identified as potentially novel species based on their 16S rDNA sequence analysis.…”

Section: Diversity Recoveredsupporting

confidence: 93%

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

Willems¹,

Paul²,

Fahime³

et al. 2012

Afr. J. Microbiol. Res.

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To study the biodiversity of halophilic bacteria in a protected wetland located in Loukkos (Northwest, Morocco), a total of 124 strains were recovered from sediment samples from a marsh and salterns. 120 isolates (98%) were found to be moderately halophilic bacteria; growing in salt ranges of 0.5 to 20%. Of 124 isolates, 102 were Gram-positive while 22 were Gram negative. All isolates were identified based on 16S rRNA gene phylogenetic analysis and characterized phenotypically and by screening for extracellular hydrolytic enzymes. The Gram-positive isolates were dominated by the genus Bacillus (89%) and the others were assigned to Jeotgalibacillus, Planococcus, Staphylococcus and Thalassobacillus. The Gram negative isolates were dominated by the genus Vibrio (41%) and the others were assigned to Halomonas, Psychrobacter, Marinobacterium, Pseudoalteromonas, Salinivibrio and Photobacterium. The growth of strains obtained under different physico-chemical conditions and the screening for hydrolytic enzymes showed a high diversity even within the same species.

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Section: Diversity Recoveredsupporting

confidence: 93%

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

Willems¹,

Paul²,

Fahime³

et al. 2012

Afr. J. Microbiol. Res.

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“…The members of the genus Bacillus are spore formers and also produce a number of biocidal metabolites/ enzymes which makes them a common inhabitant of diverse extreme habitats [26]. Sufficient evidence exists for reclassification of thermophilic bacteria in the genus Bacillus into Brevibacillus, Aneurinibacillus, Amphibacillus, Virgibacillus, Alicyclobacillus, Paenibacillus, Halobacillus and Geobacillus based on the 16S rRNA gene sequence [15].…”

Section: S Rrna Gene Sequencing and Phylogenetic Analysismentioning

confidence: 99%

“…Generally known as refractile substrates and not commonly utilized by bacteria, compounds such as D-turanose, Dglucuronic acid, glucuronamide, mucic acid, quinic acid, D-saccharic acid, formic acid and D-serine were not utilized by any of the three isolates. Isolate B31 was able to utilize maximum number of sugar derivatives [15], followed by B3 which was able to utilize 9 of the 19 sugar derivatives tested ( Table 2). Out of 19 metabolic intermediates tested, 15 were found utilized by B38 and B31 utilized 13 sugar derivatives ( Table 2).…”

Section: Biochemical Tests and Substrate Utilization Profiles For Selmentioning

confidence: 99%

“…Advances in molecular biology techniques such as sequencing a stable part of genetic code have provided an excellent opportunity for complementing the identification and characterization of bacteria at species and subspecies levels [15]. Candidate genes used for this study included as many as 20 including 5S rRNA, 16S rRNA, 23S rRNA and 16-23S rRNA internal transcribed spacer (ITS) region.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the Diversity of Culturable Thermotolerant Bacteria from Four Hot Springs of India

Yadav¹

2014

J Biodivers Biopros Dev

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The culturable bacterial diversity of four hot springs of India was analysed, employing different media and screened for temperature tolerance (40°C -70°C). Sixty morphotypes from Bakreshwar, 48 from Balrampur, 46 from Chumathang and 52 from Vashist were obtained. A set of 112 isolates, tolerant to 45°C and above, were analysed employing Amplified ribosomal DNA restriction analysis (ARDRA). Sequencing of 16S rRNA gene of the representative isolates revealed that 86%, 93%, 44% and 44% of the isolates respectively from Bakreshwar, Balrampur, Chumathang and Vashist, belonged to Firmicutes. Members of Actinobacteria were present in all the four hot springs, while Proteobacteria were present only in Chumathang and Vashist and Bacteroidetes found only in Bakreshwar. This is the first report of Aurantimonas and Brevundimonas in hot springs. Biolog analyses of three isolates, growing at or above 60°C revealed unique abilities, in terms of utilization of substrates and resistance patterns.

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“…Then, BLAST search (Altschul et al 1990) was used to compare this sequence with the one obtained from GenBank (Adiguzel et al 2009). The related database was benefited for obtaining the 16S rRNA gene sequence of the species that were considered to be like the most closely our strain.…”

Section: Characterization Of Test Bacteriamentioning

confidence: 99%

Purification and characterization of β $$ \boldsymbol{\upbeta} $$ -mannanase from Bacillus pumilus (M27) and its applications in some fruit juices

2014

Self Cite

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Thermo alkaline mannanase was purified from the bacteria of Bacillus pumilus (M27) using the techniques of ammonium sulphate precipitation, DEAE-Sephadex ion exchange chromatography and Sephacryl S200 gel filtration chromatography with 111-fold and 36 % yield. It was determined that the enzyme had 2 sub-units including 35 kDa and 55 kDa in gel filtration chromatography and SDS-PAGE electrophoresis systems. The optimum pH and temperature was determined as 8 and 60°C, respectively. It was also noticed that the enzyme did not lose its activity at a wide interval such as pH 3-11 and at high temperatures such as 90°C. Additionally, the effects of some metal ions on the mannanase enzyme activity. Moreover, the clarifying efficiency of purified mannanase enzyme with some fruit juices such as orange, apricot, grape and apple was also investigated. Enzymatic treatment was carried out with 1 mL L −1 of purified mannanase for 1 h at 60°C. It was determined that the highest pure enzyme was efficient upon clarifying the apple juice at 154 % rate.

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Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey

Cited by 106 publications

References 45 publications

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

Evaluating the Diversity of Culturable Thermotolerant Bacteria from Four Hot Springs of India

Purification and characterization of β $$ \boldsymbol{\upbeta} $$ -mannanase from Bacillus pumilus (M27) and its applications in some fruit juices

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