Identification and characterization of three novel antimicrobial peptides from Acipenser dabryanus

Chen, Yeyu; Gong, Quan; Song, Mingjiang; Lai, Jiansheng; Sun, Jiahua; Liu, Ya

doi:10.1016/j.fsi.2019.02.050

Cited by 32 publications

(12 citation statements)

References 57 publications

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“…Our findings extend recently published data of HEPC and TRFE in other Acipenser species while providing novel information on sturgeon HPX/WAP65-2, ITLN and SAA. The HEPC coding sequence that we found in A. baeri transcriptome agrees with that derived from the characterization of A. baeri HEPC gene (hamp) 70 , A. dabryanus mature HEPC 39 and two A. ruthenus hepcidin-like proteins (GenBank XM_034056050 and XM_034056903.2). Acipenser baeri hamp showed a closer genetic relationship to tetrapodian orthologs than to teleostean orthologs, suggesting that www.nature.com/scientificreports/ chondrostean HEPC may be an evolutionarily ancestral form, which would have evolved into extant hepcidins present in tetrapods and teleosts 70 .…”

Section: Discussionsupporting

confidence: 82%

“…The current data on Acipenser species reveal contrasting results for the regulation of hamp expression in the liver during infection with Gram-negative bacteria. While hamp was not upregulated after intraperitoneal bacterial challenge of A. baerii 70 or A. gueldenstaedtii (this work), it exhibited an increase in A. dabryanus challenged by bath immersion, a route that mimics natural infection 39 . These dissimilar results may be due to differences in the route of infection and/or in the pathogenicity of the bacterial strains used.…”

Section: Discussionmentioning

confidence: 58%

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Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila

Castellano

Silva-Álvarez

Aversa-Marnai

et al. 2020

Sci Rep

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The immune system of sturgeons, one of the most ancient and economically valuable fish worldwide, is poorly understood. The lack of molecular tools and data about infection biomarkers hinders the possibility to monitor sturgeon health during farming and detect infection outbreaks. To tackle this issue, we mined publicly available transcriptomic datasets and identified putative positive acute-phase proteins (APPs) of Russian sturgeons that could be induced by a bacterial infection and monitored using non-invasive methods. Teleost literature compelled us to focus on five promising candidates: hepcidin, a warm acclimation associated hemopexin, intelectin, serum amyloid A protein (SAA) and serotransferrin. Among them, SAA was the most upregulated protein at the mRNA level in the liver of sturgeons challenged with heat-inactivated or live Aeromonas hydrophila. To assess whether this upregulation yielded increasing SAA levels in circulation, we developed an in-house ELISA to quantify SAA levels in sturgeon serum. Circulating SAA rose upon bacterial challenge and positively correlated with hepatic saa expression. This is the first time serum SAA has been quantified in an Actinopterygii fish. Since APPs vary across different fish species, our work sheds light on sturgeon acute-phase response, revealing that SAA is a positive APP with potential value as infection biomarker.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 58%

Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila

Castellano

Silva-Álvarez

Aversa-Marnai

et al. 2020

Sci Rep

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“…In sturgeons, accumulations of lymphoid follicles were found in the serosa of the midgut and the spiral valve of the hindgut (F€ ange 1986; Lange et al 2000). Under bacterial infection with Edwardsiella tarda, mucosal sites (skin, gills, hindgut) acted earlier and stronger than systemic sites, as revealed by gene expression of antimicrobial peptides (AMPs) in Acipenser dabryanus (Chen et al 2019a). However, comprehensive studies on sturgeon MALTs are still required, as the currently available studies mainly refer to the gastrointestinal tract.…”

Section: Resultsmentioning

confidence: 99%

“…Shamova et al (2014) isolated and characterized six novel AMPs, called acipensins (Ac1-Ac6), from leucocytes of the octoploid Acipenser gueldenstaedtii, histone derivatives with a broad spectrum of antibacterial activity. In A. dabryanus, three novel AMPs (cathelicidin, hepcidin and defensin) were identified by transcriptome analysis and tested by infection with the gram-negative Edwardsiella tarda (Chen et al 2019a).…”

Section: Antimicrobial Peptides (Amps) and Lysozymesmentioning

confidence: 99%

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Höhne

Prokopov

Kuhl

et al. 2021

Reviews in Aquaculture

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Sturgeon immunity is relevant for basic evolutionary and applied research, including caviar-and meat-producing aquaculture, protection of wild sturgeons and their re-introduction through conservation aquaculture. Starting from a comprehensive overview of immune organs, we discuss pathways of innate and adaptive immune systems in a vertebrate phylogenetic and genomic context. The thymus as a key organ of adaptive immunity in sturgeons requires future molecular studies. Likewise, data on immune functions of sturgeon-specific pericardial and meningeal tissues are largely missing. Integrating immunological and endocrine functions, the sturgeon head kidney resembles that of teleosts. Recently identified pattern recognition receptors in sturgeon require research on downstream regulation. We review first acipenseriform data on Toll-like receptors (TLRs), type I transmembrane glycoproteins expressed in membranes and endosomes, initiating inflammation and host defence by molecular pattern-induced activation. Retinoic acid-inducible gene-I-like (RIG-like) receptors of sturgeons present RNA and key sensors of virus infections in most cell types. Sturgeons and teleosts share major components of the adaptive immune system, including B cells, immunoglobulins, major histocompatibility complex and the adaptive cellular response by T cells. The ontogeny of the sturgeon innate and onset of adaptive immune genes in different organs remain understudied. In a genomics perspective, our new data on 100 key immune genes exemplify a multitude of evolutionary trajectories after the sturgeon-specific genome duplication, where some single-copy genes contrast with many duplications, allowing tissue specialization, sub-functionalization or both. Our preliminary conclusion should be tested by future evolutionary bioinformatics, involving all >1000 immunity genes. This knowledge update about the acipenseriform immune system identifies several important research gaps and presents a basis for future applications.

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“…Antimicrobial peptides (AMPs), also known as host defense peptides, were originally referred to as a class of alkaline polypeptides with broad‐spectrum antibacterial activity induced by insects, and are known especially for their powerful action on certain drug‐resistant pathogens (Chen et al, ; Lu, ; Zhang & Gallo, ; Yeaman & Yount, ). Due to the rapid increase in drug‐resistant pathogenic microorganisms, AMPs from synthetic and natural sources had been developed as alternative antimicrobial strategies (Kang, Kim, Seo, & Park, ).…”

Section: Introductionmentioning

confidence: 99%

Microwave‐assisted extraction (MAE) combined with gas chromatography–mass spectrometry (GC–MS) for determination of volatile small molecules to evaluate compatibility of antimicrobial peptide PL‐5 spray with packaging materials

Chu

Meng

et al. 2020

Biomedical Chromatography

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A simple and efficient method involving microwave‐assisted extraction (MAE) combined with GC–MS was established to determine 1,3‐di‐tert‐butylbenzene (DBB), 2,4‐di‐tert‐butylphenol (DBP), 3,5‐di‐tert‐butyl‐4‐hydroxybenzaldehyde (DBHBA) for evaluating the compatibility of antimicrobial peptide PL‐5 spray with drug‐packaging materials. In this study, the antimicrobial peptide PL‐5 spray was purified using a Welchrom C18 column, and the high‐density polyethylene spray bottle with liquid collection tube was first mixed with absolute ethanol, which would be subjected to MAE for further measurement by GC–MS. Various experimental parameters were systematically optimized, and good linearities were obtained within the range of 0.05–1.00 μg/mL for DBB and DBHBA and 0.50–10.00 μg/mL for DBP, with limits of detection (LODs) of 0.99 ng/mL (DBB), 16.34 ng/mL (DBP), and 1.64 ng/mL (DBHBA). Satisfactory recoveries that ranged from 96.0% to 107.2% were acquired, and the relative standard deviation was ≤ 4.7%. The results showed that the maximum daily usage of DBB, DBP, and DBHBA was 9.859, 163.445, and 16.399 ng, respectively, which was far below the corresponding permitted daily exposure values according to the safety assessment, indicating that the migration of analytes did not bring any potential safety risk. The compatibility between the drug and the packaging materials was favorable.

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Identification and characterization of three novel antimicrobial peptides from Acipenser dabryanus

Cited by 32 publications

References 57 publications

Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila

Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

Microwave‐assisted extraction (MAE) combined with gas chromatography–mass spectrometry (GC–MS) for determination of volatile small molecules to evaluate compatibility of antimicrobial peptide PL‐5 spray with packaging materials

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